GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

From: 102 USD
Catalog #
75009_C
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
Products for Your Protocol

Overview

GloCell™ Fixable Viability Dye Violet 450 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.


GloCell™ Fixable Viability Dye Violet 450 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 440/40 or 440/50 band pass filter used to detect Pacific Blue™.
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry, Immunofluorescence
Brand
GloCell
Area of Interest
Angiogenic Cell Research, Antibody Development, Cancer, Cell Line Development, Cell Therapy Research, Chimerism, Disease Modeling, Drug Discovery and Toxicity Testing, Endothelial Cell Biology, Epithelial Cell Biology, HIV, HLA, Hybridoma Generation, Immunology, Infectious Diseases, Metabolism, Mitosis, Neuroscience, Organoids, Respiratory Research, Stem Cell Biology, Transplantation Research

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
75009, 75009.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
75009
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
75009.1
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Figure 1.

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Violet 450. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Violet 450.

Figure 2.

Excitation/Emission spectra for GloCell™ Fixable Viability Dye Violet 450. Excitation max: 403 nm, Emission Max: 454 nm

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