Hematopoietic Stem and Progenitor Cell Research
Hematopoietic stem and progenitor cells (HSPCs) can be identified both phenotypically, e.g., by flow cytometry, and functionally, with colony-forming unit (CFU) or long-term culture-initiating cell (LTC-IC) assays.
Products
Cell Culture Media and Supplements (4 43)
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MegaCult™-C Complete Kit Without Cytokines
Complete kit for human CFU-Mk assays -
MethoCult™ SF H4636 Without EPO
Serum-free methylcellulose-based medium with recombinant cytokines for human hematopoietic cells -
MegaCult™-C Medium with Lipids
Medium with lipids, without cytokines, for human and mouse CFU-Mk assays -
MegaCult™-C Collagen and Medium with Lipids
Kit containing collagen and medium with lipids, without cytokines, for human and mouse CFU-Mk assays
Instruments and Software (4 2)
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STEMvision™
Automated and standardized colony counting for the hematopoietic colony-forming unit (CFU) assay -
L-Calc™ Software
Software for limiting dilution analysis
Cytokines and Proteins (4 2)
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Human Recombinant FGF-acidic, ACF
Fibroblast growth factor-acidic, animal component-free -
Human Recombinant FGF-acidic
Fibroblast growth factor-acidic
Antibodies (4 29)
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Anti-Human CD45 Antibody, Clone 2D1
Mouse monoclonal IgG1 antibody against human CD45 -
Anti-Human CD45RA, Clone HI100
Mouse monoclonal IgG2b antibody against human, chimpanzee CD45RA -
Anti-Mouse CD48 (SLAMF2) Antibody, Clone HM48-1
Hamster (Armenian) monoclonal IgG1 antibody against mouse CD48 (SLAMF2) -
Anti-Mouse CD43 Antibody, Clone R2/60
Rat monoclonal IgM antibody against mouse CD43
Cell Dyes and Detection Assay Kits (4 16)
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Annexin V Apoptosis Detection Kit with 7-AAD
Apoptosis staining and detection kit -
Annexin V Binding Buffer
Assay buffer for labeling with Annexin V -
Annexin V
A cellular protein for detection of apoptotic cells -
GloCell™ Fixable Viability Dye Violet 540
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
Cultureware and General Supplies (4 11)
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245 mm Square Dish, Tissue Culture-Treated
Sterile clear polystyrene tissue culture-treated dish with lid -
SmartDish™
Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays -
Double Chamber Slide Kit
For the culture, fixation, and staining of collagen-based cultures -
STEMgrid™-6
Detachable grid for manual counting of hematopoietic colonies in SmartDish™ 6-well plates using an inverted microscope
Buffers and Solutions (4 5)
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Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
Cell culture buffer -
ALDEFLUOR™ Assay Buffer
Assay buffer for labeling with the ALDEFLUOR™ kit -
HBSS with 10 mM HEPES, Without Phenol Red
Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red -
HBSS, Modified (Without Ca++ and Mg++)
Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium
Small Molecules (4 1)
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Hydrocortisone
Enhances proliferation and differentiation of endothelial, epithelial, mesenchymal, and oligodendrocyte cells
Training and Education (4 3)
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Applications of the Hematopoietic Progenitor Assay Training Course
Three-day hands-on training on the CFU Assay for human, mouse, and rat samples -
Standardization of the Hematopoietic Progenitor Assay Training Course
Two-day hands-on training on the CFU Assay for human cord blood and bone marrow samples -
Pluripotent Stem Cell Training
Training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors
Standardization Tools (4 8)
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Contract Assay Services
Contract Research Organization (CRO) at STEMCELL Technologies -
Human Hematopoietic Colonies in Health and Disease
Atlas of human hematopoietic colonies from healthy and diseased states -
Atlas of Human Hematopoietic Colonies
Reference guide for identifying hematopoietic colonies in methylcellulose-based medium -
Frozen Cord Blood Proficiency Testing Program
For the hematopoietic colony forming unit (CFU) assay
Matrices and Substrates (4 1)
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Collagen Solution
For preparation of collagen gels and for coating cell culture surfaces
ELISAs (4 1)
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Erythropoietin (EPO) ELISA Kit
Immunoassay for detection and measurement of human erythropoietin