Hematopoietic Stem and Progenitor Cell Research

Hematopoietic stem and progenitor cells (HSPCs) can be identified both phenotypically, e.g., by flow cytometry, and functionally, with colony-forming unit (CFU) or long-term culture-initiating cell (LTC-IC) assays. See MoreCFU assays are an important tool when assaying changes in progenitor cell frequency after HSPC expansion with novel small molecules, or following manipulation of cells with CRISPR-Cas9. To standardize CFU assays of human and mouse cells, plate cells in highly robust semi-solid culture media , and count hematopoietic colonies using automated imaging and analysis software . Perform analysis with antibodies for flow cytometry , to identify HSPC populations present after culture, or to measure engraftment following transplantation into immunocompromised mice when performing in vivo engraftment assays.