Hematopoietic Stem and Progenitor Cell Research

Hematopoietic stem and progenitor cells (HSPCs) can be identified both phenotypically, e.g., by flow cytometry, and functionally, with colony-forming unit (CFU) or long-term culture-initiating cell (LTC-IC) assays. See MoreCFU assays are an important tool when assaying changes in progenitor cell frequency after HSPC expansion with novel small molecules, or following manipulation of cells with CRISPR-Cas9. To standardize CFU assays of human and mouse cells, plate cells in highly robust semi-solid culture media , and count hematopoietic colonies using automated imaging and analysis software . Perform analysis with antibodies for flow cytometry , to identify HSPC populations present after culture, or to measure engraftment following transplantation into immunocompromised mice when performing in vivo engraftment assays.

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Cell Culture Media and Supplements 4 (45)

Instruments and Software 4 (1)

  • STEMvision™

    Automated and standardized colony counting for the hematopoietic colony-forming unit (CFU) assay

Cytokines and Proteins 4 (2)

Antibodies 4 (29)

Cell Dyes and Detection Assay Kits 4 (17)

Cultureware and General Supplies 4 (10)

Buffers and Solutions 4 (5)

Small Molecules 4 (1)

  • Hydrocortisone

    Enhances proliferation and differentiation of endothelial, epithelial, mesenchymal, and oligodendrocyte cells

Training and Education 4 (4)

Standardization Tools 4 (7)

Laboratory Equipment 4 (2)

Matrices and Substrates 4 (1)

ELISAs 4 (1)