Hematopoietic Stem and Progenitor Cell Research
Hematopoietic stem and progenitor cells (HSPCs) can be identified both phenotypically, e.g., by flow cytometry, and functionally, with colony-forming unit (CFU) or long-term culture-initiating cell (LTC-IC) assays.
Products
Cell Culture Media and Supplements (4 12)
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MethoCult™ H4034 Optimum
Methylcellulose-based medium with recombinant cytokines for human cells -
Starter Kit for MethoCult™ H4034 Optimum
Complete kit for hematopoietic CFU assays -
MethoCult™ H4035 Optimum Without EPO
Methylcellulose-based medium with recombinant cytokines (without erythropoietin [EPO]) for human cells -
MethoCult™ H4434 Classic
Methylcellulose-based medium with recombinant cytokines for human cells
Antibodies (4 9)
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Anti-Human CD3 Antibody, Clone UCHT1
Mouse (BALB/c) monoclonal IgG1 antibody against human, chimpanzee CD3 -
Anti-Mouse CD11b Antibody, Clone M1/70
Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b -
Anti-Human CD11b Antibody, Clone ICRF44
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD11b -
Anti-Human CD16 Antibody, Clone 3G8
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD16
Cell Dyes and Detection Assay Kits (4 12)
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GloCell™ Fixable Viability Dye Violet 540
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 510
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye Violet 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells -
GloCell™ Fixable Viability Dye UV 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
Cultureware and General Supplies (4 9)
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245 mm Square Dish, Tissue Culture-Treated
Sterile clear polystyrene tissue culture-treated dish with lid -
SmartDish™
Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays -
STEMgrid™-6
Detachable grid for manual counting of hematopoietic colonies in SmartDish™ 6-well plates using an inverted microscope -
Blunt-End Needles, 16 Gauge
16 gauge needles with luer lock
Buffers and Solutions (4 5)
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Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
Cell culture buffer -
ALDEFLUOR™ Assay Buffer
Assay buffer for labeling with the ALDEFLUOR™ kit -
HBSS with 10 mM HEPES, Without Phenol Red
Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red -
HBSS, Modified (Without Ca++ and Mg++)
Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium
Small Molecules (4 1)
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Hydrocortisone
Enhances proliferation and differentiation of endothelial, epithelial, mesenchymal, and oligodendrocyte cells
Matrices and Substrates (4 1)
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Collagen Solution
For preparation of collagen gels and for coating cell culture surfaces