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Data and Publications
(A) The biological activity of Human Recombinant FGF-acidic, ACF was tested by its ability to promote proliferation of NR6R-3T3 cells in the presence of 10 μg/mL heparin. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.0277 ng/mL. (B) 1 μg of Human Recombinant FGF-acidic, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant FGF-acidic, ACF has a predicted molecular mass of 16.0 kDa.