Human Recombinant FGF-acidic, ACF

Fibroblast growth factor-acidic, animal component-free
Catalog #
78188_C
Fibroblast growth factor-acidic, animal component-free
From: 97 USD

Overview

Fibroblast growth factor acidic (FGF-acidic), also known as FGF-1, is a potent activator of DNA synthesis, cell proliferation, and chemotaxis and is known to play numerous roles in development, regeneration, and angiogenesis (Galzie et al.; Jaye et al.; Presta et al.). FGF-acidic is produced by multiple cell types and is capable of activating all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It is found in large quantities in the brain, but is also expressed in hepatocytes, vascular smooth muscle cells, neurons of the central nervous system, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelial cells, and pituitary basophils and acidophils. FGF-acidic is secreted as a disulfide-linked homodimer and is stored in complex with heparan sulfate, a requirement for its interaction with FGF receptors (Guerrini et al.; Mohammadi et al.). Internalized FGF-acidic signals via protein kinase C and promotes cell survival by inhibiting p53 and proapoptotic signaling (Bouleau et al.). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Alternative Names
Acidic fibroblast growth factor, aFGF, ECGF, Endothelial cell growth factor, FGF-1, FGF-alpha, Fibroblast growth factor 1, GLIO703, HBGF-1, Heparin-binding growth factor 1
Cell Type
Endoderm, PSC-Derived, Endothelial Cells, Epithelial Cells, Fibroblasts, Granulocytes and Subsets, Hepatic Cells, Keratinocytes, Mesoderm, PSC-Derived, Neurons, Smooth Muscle
Species
Human
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
16.0 kDa
Purity
≥ 95%

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant FGF-acidic, ACF
Catalog #
78188, 78188.1, 78188.2, 78188.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant FGF-acidic, ACF
Catalog #
78188, 78188.1, 78188.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant FGF-acidic, ACF
Catalog #
78188.3
Lot #
All
Language
English

Educational Materials(5)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Small Molecules, Big Impact in Pluripotent Stem Cell Research

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant FGF-acidic, ACF was tested by its ability to promote proliferation of NR6R-3T3 cells in the presence of 10 μg/mL heparin. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.0277 ng/mL. (B) 1 μg of Human Recombinant FGF-acidic, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant FGF-acidic, ACF has a predicted molecular mass of 16.0 kDa.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.