Neuronal Research

Primary neuronal culture is an important tool for many areas of neuroscience research. See MoreTo obtain a healthy culture with good morphology and cellular function, use of high-quality media and supplements is crucial. BrainPhys™ is a new, defined and serum-free neuronal culture medium for the culture of primary neurons and human pluripotent stem cell-derived neurons. Based on the formulation published by Cedric Bardy and Fred H. Gage (C Bardy et al. Proc Natl Acad Sci USA, 2015), BrainPhys™ is more representative of the CNS extracellular environment and increases the proportion of synaptically active neurons.

Lot-to-lot variability in commercial neural supplements can cause inconsistent results. At STEMCELL Technologies, we rigorously pre-screen the components of all media and supplements and subject the finished products to extensive quality control testing. Our SM Neuronal Supplements are based on Brewer’s B27 supplement (Brewer et al. 1993), but optimized to more consistently support the growth of mature, functional neurons. Neurons cultured using NeuroCult™ SM1 Neuronal Supplement show increased cell survival, neurite outgrowth and neurite branching.