Due to difficulties associated with obtaining human brain tissue for neuroscience
research, mice and rats are widely used as model organisms. See MoreHowever, obtaining viable neurons for culture can be technically challenging. Begin by dissociating central nervous system (CNS) tissue to a single-cell suspension.
Trypsin-EDTA
is a versatile dissociation reagent that is suitable for use with embryonic and early postnatal mouse and rat tissue. For another option for some protocols, we recommend using link papain as the enzymatic dissociation reagent.
Whether you’re starting from primary cells, tissue samples, or pluripotent stem cells, explore our complete portfolio of products to support your neural culture workflow.