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Neuronal Research

Due to difficulties associated with obtaining human brain tissue for neuroscience research, mice and rats are widely used as model organisms. See MoreHowever, obtaining viable neurons for culture can be technically challenging. Begin by dissociating central nervous system (CNS) tissue to a single-cell suspension. Trypsin-EDTA is a versatile dissociation reagent that is suitable for use with embryonic and early postnatal mouse and rat tissue. For another option for some protocols, we recommend using link papain as the enzymatic dissociation reagent.

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