NeuroCult™ Neural Stem Cell Culture Media
NeuroCult™ Neural Cell Culture Media
For Primary Neuronal and Neural Stem Cell Culture
NeuroCult™ is a line of culture media and reagents formulated to provide optimal culture conditions for
,
, and
. NeuroCult™ allows researchers to standardize their culture system and minimize variability, leading to increased reproducibility among experiments.
Why Use NeuroCult™?
- Media, supplements and accessory reagents are available for primary neurons and neural stem/progenitor cells from human, mouse and rat tissues.
- Isolate, expand, and differentiate mouse and rat neural stem and progenitor cells from normal and tumor tissues.
- Culture and expand human neural stem cells and brain tumor stem cells in a serum-free and/or xeno-free system.
- Rigorous raw material screening and quality control ensure minimal lot-to-lot variability.
For the generation and culture of neural cells derived from human pluripotent stem cells (hPSCs; including embryonic stem (ES) cell and induced pluripotent stem (iPS) cells), see and , which are part of the workflow.
NeuroCult™ for CNS-Derived Mouse and Rat Neural Stem and Progenitor Cells
NeuroCult™ Proliferation Kits
Species:
Mouse or Rat
Formulation:
Defined and serum-free
Applications:
- Culture and expansion of embryonic and adult neural stem and progenitor cells, in the neurosphere or adherent monolayer system
Features/Advantages:
Efficient expansion of neural stem and progenitor cells without loss of self-renewal, proliferation or differentiation potential
Notes:
Must be supplemented as follows:
- Embryonic mouse: rh EGF
- Adult mouse: rh EGF, rh FGF and heparin
- Embryonic rat: rh EGF, rh FGF and heparin
NeuroCult™ Differentiation Kits
Species:
Mouse or Rat
Formulation:
Serum-containing
Applications:
- In vitro differentiation of embryonic and adult neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes
Features/Advantages:
Efficient and reproducible differentiation to neurons, astrocytes and oligodendrocytes
NeuroCult™ Dissociation Kits
Species:
Mouse (Chemical Dissociation); Mouse and Rat (Enzymatic Dissociation)
Formulation:
Defined and serum-free
Applications:
- Use NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) to obtain a single-cell suspension from CNS tissue
- Use NeuroCult™ Chemical Dissociation Kit (Mouse) for the non-mechanical and non-enzymatic dissociation of neurospheres derived from embryonic and adult mouse CNS cells
Features/Advantages:
Fast, reproducible and yields high total cell numbers and good cell viability
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NeuroCult™ SM1 for Primary and hPSC-Derived Neurons
NeuroCult™ SM1 Neuronal Supplement
Species:
Mouse and Rat Primary Neurons, Human ES/iPS Cell-Derived Neurons
Formulation:
Defined and serum-free supplement based on Brewer’s B271
Applications:
- Culture of primary mouse and rat neurons
- Differentiation and maturation of human ES/iPS cell-derived neurons*
- Versatile serum-replacement supplement for various applications
Features/Advantages:
- Formulated to support improved cell survival in long-term primary neuronal culture
- Cultures feature increased neurite outgrowth and branching in short- and long-term cultures
- Product undergoes rigorous performance testing
Notes:
Also available:
NeuroCult™ SM1 Without Vitamin A
*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit .
NeuroCult™ SM1 Without Vitamin A
*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit .
BrainPhys™ Neuronal Medium and SM1 Kit
Species:
Mouse and Rat
Formulation:
Defined and serum-free basal medium and supplement
BrainPhys™ Neuronal Medium is based on the formulation invented by Cedric Bardy and Fred H. Gage2
BrainPhys™ Neuronal Medium is based on the formulation invented by Cedric Bardy and Fred H. Gage2
Applications:
- Neurophysiological culture of primary mouse and rat neurons
- Differentiation and maturation of functionally active human ES/iPS cell-derived neurons*
- Microelectrode array-based recording of neuronal activity
Features/Advantages:
- Complete kit formulated to support improved neuronal function in long-term primary and human ES/iPS cell-derived neuronal cultures
- Cultures experience brain-like physiological conditions and have a higher proportion of synaptically active neurons
- Perform functional assays without replacing media
Notes:
Kit includes
BrainPhys™ Neuronal Medium
and
NeuroCult™ SM1 Neuronal Supplement
*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit .
*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit .
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NeuroCult™ for Human Neural Stem Cells and Brain Tumor Stem Cells
NeuroCult™ NS-A Proliferation Kit
Species:
Human
Formulation:
Defined and serum-free
Applications:
Culture and expansion of human neural stem and progenitor cells or brain tumor stem cells as neurospheres or adherent monolayers
Features/Advantages:
Efficient expansion in culture without loss of self-renewal, proliferation or differentiation potential
Notes:
Must be supplemented with:
- rh EGF
- rh bFGF
- Heparin
NeuroCult™ Xeno-Free Proliferation Medium
Species:
Human
Formulation:
Defined, xeno-free and serum-free
Applications:
Culture and expansion of human neural stem and progenitor cells or brain tumor stem cells as neurospheres or adherent monolayers
Features/Advantages:
Efficient expansion in culture without loss of self-renewal, proliferation or differentiation potential
Notes:
Must be supplemented with:
- rh EGF
- rh bFGF
- Heparin
NeuroCult™ NS-A Differentiation Kit
Species:
Human
Formulation:
Serum-containing
Applications:
Differentiation of human neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes
Features/Advantages:
Efficient and reproducible differentiation to neurons, astrocytes and oligodendrocytes
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Antibody Finder Tool
Find the Right Antibody for Your Applications
Don't waste valuable research time finding the appropriate antibody for your cell analysis. Use our Antibody Finder Tool to select high-quality primary and secondary antibodies verified to work with NeuroCult™ products in specific applications.
View Tool >Scientific Resources
Brochure
Neural Supplements For High-Quality Neural Cell Cultures
Brochure
Primary Neuronal Culture: Standardized Media and Reagents
References
- Brewer GJ et al. (1993) Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J Neurosci Res. 35(5):567-76.
- Bardy C et al. (2015) Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro. Proc Natl Acad Sci 112 (20) E2725-E2734.
