NeuroCult™

For Primary Neuronal and Neural Stem Cell Culture

NeuroCult™ is a line of culture media and reagents formulated to provide optimal culture conditions for , , and . NeuroCult™ allows researchers to standardize their culture system and minimize variability, leading to increased reproducibility among experiments.

Why Use NeuroCult™?

COMPREHENSIVE. Media, supplements and accessory reagents are available for primary neurons and neural stem/progenitor cells from human, mouse and rat tissues.
COMPLETE WORKFLOW. Isolate, expand, and differentiate mouse and rat neural stem and progenitor cells from normal and tumor tissues.
VERSATILE. Culture and expand human neural stem cells and brain tumor stem cells in a serum-free and/or xeno-free system.
RELIABLE. Rigorous raw material screening and quality control ensure minimal lot-to-lot variability.

For the generation and culture of neural cells derived from human pluripotent stem cells (hPSCs; including embryonic stem (ES) cell and induced pluripotent stem (iPS) cells), see STEMdiff™ and BrainPhys™, which are part of the hPSC-derived neural cell research workflow.

NeuroCult™ for CNS-Derived Mouse and Rat Neural Stem Cells NeuroCult™ for Primary hPSC-Derived Neurons NeuroCult™ for Human Neural and Brain Tumor Stem Cells

NeuroCult™ for CNS-Derived Mouse and Rat Neural Stem and Progenitor Cells

NeuroCult™ Proliferation Kits

Species:

Mouse or Rat

Formulation:

Defined and serum-free

Applications:

Culture and expansion of embryonic and adult neural stem and progenitor cells, in the neurosphere or adherent monolayer system

Features/Advantages:

Efficient expansion of neural stem and progenitor cells without loss of self-renewal, proliferation or differentiation potential

Notes:

Must be supplemented as follows:

Embryonic mouse: rh EGF
Adult mouse: rh EGF, rh FGF and heparin
Embryonic rat: rh EGF, rh FGF and heparin

NeuroCult™ Differentiation Kits

Species:

Mouse or Rat

Formulation:

Serum-containing

Applications:

In vitro differentiation of embryonic and adult neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes

Features/Advantages:

Efficient and reproducible differentiation to neurons, astrocytes and oligodendrocytes

NeuroCult™ Dissociation Kits

Species:

Mouse (Chemical Dissociation); Mouse and Rat (Enzymatic Dissociation)

Formulation:

Defined and serum-free

Applications:

Use NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) to obtain a single-cell suspension from CNS tissue
Use NeuroCult™ Chemical Dissociation Kit (Mouse) for the non-mechanical and non-enzymatic dissociation of neurospheres derived from embryonic and adult mouse CNS cells

Features/Advantages:

Fast, reproducible and yields high total cell numbers and good cell viability

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NeuroCult™ SM1 for Primary and hPSC-Derived Neurons

NeuroCult™ SM1 Neuronal Supplement

Species:

Mouse and Rat Primary Neurons, Human ES/iPS Cell-Derived Neurons

Formulation:

Defined and serum-free supplement based on Brewer’s B27¹

Applications:

Culture of primary mouse and rat neurons
Differentiation and maturation of human ES/iPS cell-derived neurons*
Versatile serum-replacement supplement for various applications

Features/Advantages:

Formulated to support improved cell survival in long-term primary neuronal culture
Cultures feature increased neurite outgrowth and branching in short- and long-term cultures
Product undergoes rigorous performance testing

Notes:

Also available:
NeuroCult™ SM1 Without Vitamin A

*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit.

BrainPhys™ Neuronal Medium and SM1 Kit

Species:

Mouse and Rat

Formulation:

Defined and serum-free basal medium and supplement

BrainPhys™ Neuronal Medium is based on the formulation invented by Cedric Bardy and Fred H. Gage²

Applications:

Neurophysiological culture of primary mouse and rat neurons
Differentiation and maturation of functionally active human ES/iPS cell-derived neurons*
Microelectrode array-based recording of neuronal activity

Features/Advantages:

Complete kit formulated to support improved neuronal function in long-term primary and human ES/iPS cell-derived neuronal cultures
Cultures experience brain-like physiological conditions and have a higher proportion of synaptically active neurons
Perform functional assays without replacing media

Notes:

Kit includes BrainPhys™ Neuronal Medium and NeuroCult™ SM1 Neuronal Supplement

*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit.

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NeuroCult™ for Human Neural Stem Cells and Brain Tumor Stem Cells

NeuroCult™ NS-A Proliferation Kit

Species:

Human

Formulation:

Defined and serum-free

Applications:

Culture and expansion of human neural stem and progenitor cells or brain tumor stem cells as neurospheres or adherent monolayers

Features/Advantages:

Efficient expansion in culture without loss of self-renewal, proliferation or differentiation potential

Notes:

Must be supplemented with:

rh EGF
rh bFGF
Heparin

NeuroCult™ Xeno-Free Proliferation Medium

Species:

Human

Formulation:

Defined, xeno-free and serum-free

Applications:

Culture and expansion of human neural stem and progenitor cells or brain tumor stem cells as neurospheres or adherent monolayers

Features/Advantages:

Efficient expansion in culture without loss of self-renewal, proliferation or differentiation potential

Notes:

Must be supplemented with:

rh EGF
rh bFGF
Heparin

NeuroCult™ NS-A Differentiation Kit

Species:

Human

Formulation:

Serum-containing

Applications:

Differentiation of human neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes

Features/Advantages:

Efficient and reproducible differentiation to neurons, astrocytes and oligodendrocytes

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References

Brewer GJ et al. (1993) Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J Neurosci Res. 35(5):567-76.

Bardy C et al. (2015) Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro. Proc Natl Acad Sci 112 (20) E2725-E2734.

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