NeuroCult™
NeuroCult™ Neural Cell Culture Media
For Primary Neuronal and Neural Stem Cell Culture
NeuroCult™ is a line of culture media and reagents formulated to provide optimal culture conditions for , , and . NeuroCult™ allows researchers to standardize their culture system and minimize variability, leading to increased reproducibility among experiments.
Why Use NeuroCult™?
- Media, supplements and accessory reagents are available for primary neurons and neural stem/progenitor cells from human, mouse and rat tissues.
- Isolate, expand, and differentiate mouse and rat neural stem and progenitor cells from normal and tumor tissues.
- Culture and expand human neural stem cells and brain tumor stem cells in a serum-free and/or xeno-free system.
- Rigorous raw material screening and quality control ensure minimal lot-to-lot variability.
For the generation and culture of neural cells derived from human pluripotent stem cells (hPSCs; including embryonic stem (ES) cell and induced pluripotent stem (iPS) cells), see and , which are part of the workflow.
NeuroCult™ for CNS-Derived Mouse and Rat Neural Stem and Progenitor Cells
NeuroCult™ Proliferation Kits
Species:
Mouse or Rat
Formulation:
Defined and serum-free
Applications:
- Culture and expansion of embryonic and adult neural stem and progenitor cells, in the neurosphere or adherent monolayer system
Features/Advantages:
Efficient expansion of neural stem and progenitor cells without loss of self-renewal, proliferation or differentiation potential
Notes:
Must be supplemented as follows:
- Embryonic mouse: rh EGF
- Adult mouse: rh EGF, rh FGF and heparin
- Embryonic rat: rh EGF, rh FGF and heparin
NeuroCult™ Differentiation Kits
Species:
Mouse or Rat
Formulation:
Serum-containing
Applications:
- In vitro differentiation of embryonic and adult neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes
Features/Advantages:
Efficient and reproducible differentiation to neurons, astrocytes and oligodendrocytes
NeuroCult™ Dissociation Kits
Species:
Mouse (Chemical Dissociation); Mouse and Rat (Enzymatic Dissociation)
Formulation:
Defined and serum-free
Applications:
- Use NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) to obtain a single-cell suspension from CNS tissue
- Use NeuroCult™ Chemical Dissociation Kit (Mouse) for the non-mechanical and non-enzymatic dissociation of neurospheres derived from embryonic and adult mouse CNS cells
Features/Advantages:
Fast, reproducible and yields high total cell numbers and good cell viability
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NeuroCult™ SM1 for Primary and hPSC-Derived Neurons
NeuroCult™ SM1 Neuronal Supplement
Species:
Mouse and Rat Primary Neurons, Human ES/iPS Cell-Derived Neurons
Formulation:
Defined and serum-free supplement based on Brewer’s B271
Applications:
- Culture of primary mouse and rat neurons
- Differentiation and maturation of human ES/iPS cell-derived neurons*
- Versatile serum-replacement supplement for various applications
Features/Advantages:
- Formulated to support improved cell survival in long-term primary neuronal culture
- Cultures feature increased neurite outgrowth and branching in short- and long-term cultures
- Product undergoes rigorous performance testing
Notes:
Also available:
NeuroCult™ SM1 Without Vitamin A
*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit.
NeuroCult™ SM1 Without Vitamin A
*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit.
BrainPhys™ Neuronal Medium and SM1 Kit
Species:
Mouse and Rat
Formulation:
Defined and serum-free basal medium and supplement
BrainPhys™ Neuronal Medium is based on the formulation invented by Cedric Bardy and Fred H. Gage2
BrainPhys™ Neuronal Medium is based on the formulation invented by Cedric Bardy and Fred H. Gage2
Applications:
- Neurophysiological culture of primary mouse and rat neurons
- Differentiation and maturation of functionally active human ES/iPS cell-derived neurons*
- Microelectrode array-based recording of neuronal activity
Features/Advantages:
- Complete kit formulated to support improved neuronal function in long-term primary and human ES/iPS cell-derived neuronal cultures
- Cultures experience brain-like physiological conditions and have a higher proportion of synaptically active neurons
- Perform functional assays without replacing media
Notes:
Kit includes BrainPhys™ Neuronal Medium and NeuroCult™ SM1 Neuronal Supplement
*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit.
*See also: STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit.
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NeuroCult™ for Human Neural Stem Cells and Brain Tumor Stem Cells
NeuroCult™ NS-A Proliferation Kit
Species:
Human
Formulation:
Defined and serum-free
Applications:
Culture and expansion of human neural stem and progenitor cells or brain tumor stem cells as neurospheres or adherent monolayers
Features/Advantages:
Efficient expansion in culture without loss of self-renewal, proliferation or differentiation potential
Notes:
Must be supplemented with:
- rh EGF
- rh bFGF
- Heparin
NeuroCult™ Xeno-Free Proliferation Medium
Species:
Human
Formulation:
Defined, xeno-free and serum-free
Applications:
Culture and expansion of human neural stem and progenitor cells or brain tumor stem cells as neurospheres or adherent monolayers
Features/Advantages:
Efficient expansion in culture without loss of self-renewal, proliferation or differentiation potential
Notes:
Must be supplemented with:
- rh EGF
- rh bFGF
- Heparin
NeuroCult™ NS-A Differentiation Kit
Species:
Human
Formulation:
Serum-containing
Applications:
Differentiation of human neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes
Features/Advantages:
Efficient and reproducible differentiation to neurons, astrocytes and oligodendrocytes
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Scientific Resources
References
- Brewer GJ et al. (1993) Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J Neurosci Res. 35(5):567-76.
- Bardy C et al. (2015) Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro. Proc Natl Acad Sci 112 (20) E2725-E2734.