NeuroCult™-XF Proliferation Medium
Xeno-free medium for expansion of human neural stem and progenitor cells
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Overview
NOTE: Addition of Carrier-Free rh EGF (Catalog #78006) and rh bFGF (Catalog #78003) is required. Heparin (Catalog #07980) can also be added, however, the heparin solution contains non-human animal-derived components and can be omitted if a complete xeno-free system is desired.
Data Figures

Figure 1. Neurospheres Derived from Human Fetal CNS Tissue can be Efficiently Generated for Multiple Passages in NeuroCult™-XF Proliferation Medium (n = 3)
Total cell expansion obtained with NeuroCult™-XF is comparable to that obtained with NeuroCult™ NS-A Proliferation Medium (Human).

Figure 2. NSCs Cultured in NeuroCult™-XF Proliferation Medium Maintain Multi-Lineage Differentiation Potential in Long-Term Cultures
The graph indicates the percentage of neurons (class III ß-tubulin-positive), astrocytes (GFAP-positive) and oligodendrocytes (O4-positive) generated following differentiation of NSCs previously cultured in NeuroCult™-XF or NeuroCult™ NS-A Proliferation Medium (Human). The differentiation potential of NSCs cultured in NeuroCult™-XF is comparable to NSCs cultured in NeuroCult™ NS-A Proliferation Medium (Human).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (11)
Publications (3)
Abstract
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Legal Statement:
Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.