Register: On-Demand Mouse HSPCs CFU Assay Course
Sign up for an on-demand course to learn best practices for performing the CFU assay for mouse-derived HSPCs
Instructor Bios
Dr. Jackie Damen, Principal Scientist, Scientific Support, Hematology
Dr. Jackie Damen is the Senior Scientific Advisor of the Hematopoiesis department at STEMCELL Technologies. Dr. Damen has extensive experience in the field of hematopoiesis, where her key strengths lie in stem cell biology, protein biochemistry, and cell signaling, especially in the area of erythropoietin. In her role as Senior Scientific Advisor, Hematopoiesis, as well as her past experience as Director of Contract Assay Services at STEMCELL, Dr. Damen has conducted hundreds of customized studies involving the CFU assay to provide pharmaceutical and biotechnology clients with solutions to drug development questions. Dr. Damen actively participates in educational initiatives, regularly providing online talks and presentations at high-profile conferences, such as the Society of Toxicology Annual Meeting and the International Society of Cellular Therapy (ISCT). At STEMCELL, Dr. Damen has been involved in customer training in hematopoietic stem cell biology and colony identification since 2002.
Dr. Tina Jeng, Scientist, Scientific Support, Hematology
Course Curriculum
Critical characteristics of hematopoietic stem vs. progenitor cells and the basic biology of mouse hematopoiesis.
Mini Quiz: Test Your Knowledge
Estimated time for completion: 20 minutes
- Part 1: Phenotypic assays
- Part 2: Functional assays
Mini Quiz: Test Your Knowledge
Estimated time for completion: 30 minutes
- Part 1: Choosing a source of mouse HSPCs
- Part 2: Sample collection and preparation
- Part 3: Choosing a MethoCult™ formulation
- Part 4: CFU assay protocol and analysis overview
- Part 5: Standardization of the CFU assay
Mini Quiz: Test Your Knowledge
Virtual Lab: Step-by-step narrated “How to Set Up the CFU Assay for Custom Hematopoietic Training Courses Using SmartDish™”
Estimated time for completion: 60 minutes
- Part 1: Customizing the CFU assay for your application
- Part 2: Optimizing the CFU assay protocol
- Part 3: Case study
Mini Quiz: Test Your Knowledge
Estimated time for completion: 35 minutes
- Part 1: Customizing the CFU assay for your application
- Part 2: Optimizing the CFU assay protocol
- Part 3: Case study
Mini Quiz: Test Your Knowledge
Estimated time for completion: 50 minutes
- Part 1: Automation of the mouse CFU assay using STEMvision™
- Part 2: Scoring mouse CFU: Results from STEMvision™
- Part 3: Applications of STEMvision™ mouse analysis packages
Mini Quiz: Test Your Knowledge
Virtual Lab: Step-by-step narrated “How To” lab video demonstrations for STEMvision™
Estimated time for completion: 50 minutes
Complete this final assessment to test your knowledge and receive a certificate following successful completion.
