NeuroCult™ SM1 Neuronal Supplement
Supplement (50X) for the serum-free culture of neurons
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L-GlutamineCell culture supplement
Overview
NeuroCult™ SM1 (STEMCELL Modified-1) Neuronal Supplement is based on the published formulation (Brewer et al. Brain Res, 1989) and standardized to more reproducibly support survival and maturation of functional neurons. Cultures with this serum-free supplement feature increased neurite outgrowth and branching in short- and long-term cultures. NeuroCult™ SM1 may also be used as a serum-replacement supplement for various customizable applications, such as neurotoxicity assays and calcium imaging.
For your convenience, NeuroCult™ SM1 is included as a component of multiple BrainPhys™ Neuronal Medium culture kits for primary and hPSC-derived neurons (Catalog #05792, 05793, 05794, and 05795). For further details, see the performance data with BrainPhys™ below.
• Vitamin A
• Insulin
• Other ingredients
Data Figures
Figure 1. Protocol for Plating and Culturing Primary Neurons with the SM1 Culture System
Primary rodent tissue dissociated in papain was plated in NeuroCult™ Neuronal Plating Medium, supplemented with NeuroCult™ SM1 Neuronal Supplement, L-Glutamine, and L-Glutamic Acid. On day 5, primary neurons were transitioned to BrainPhys™ Neuronal Medium, supplemented with NeuroCult™ SM1 Neuronal Supplement, by performing half-medium changes every 3 - 4 days.
Figure 2. The SM1 Culture System Supports Long-Term Culture of Rodent Neurons
Primary E18 rat cortical neurons were cultured in the SM1 Culture System. A large number of viable neurons are visible after (A) 21 and (B) 35 days, as demonstrated by their bright neuronal cell bodies, and extensive neurite outgrowth and branching. Neurons are evenly distributed over the culture surface with minimal cell clumping.
Figure 3. Pre- and Post-Synaptic Markers are Expressed in Rodent Neurons Cultured in the SM1 Culture System
Primary E18 rat cortical neurons were cultured in the SM1 Culture System. At 21 DIV, neurons are phenotypically mature, as indicated by the presence of an extensive dendritic arbor, and appropriate expression and localization of pre-synaptic synapsin (A,C; green) and post-synaptic PSD-95 (A,B; red) markers. Synapsin is concentrated in discrete puncta distributed along the somata and dendritic processes, as defined by the dendritic marker MAP2 (A,D; blue).
Figure 4. The SM1 Culture System Supports Increased Cell Survival
(A) Primary E18 rat cortical neurons were cultured in the SM1 Culture System or a Competitor Culture System for 21 days. Neurons cultured in the SM1 Culture System have a significantly higher number of viable cells compared to the competitor culture system (n = 4; mean ± 95% CI; *p < 0.05). (B) Primary E18 rat cortical neurons were cultured in Neurobasal® supplemented with NeuroCult™ SM1 Neuronal Supplement (SM1) or competitor B27-like supplements (Competitor 1,2,3) for 21 days. Cultures supplemented with NeuroCult™ SM1 Neuronal Supplement have an equal number of neurons compared to competitor-supplemented cultures. Bars represent standard error of mean.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (14)
Publications (50)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
