hPSC-Derived Neural Cell Research
Human pluripotent stem cells (hPSCs), including human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are self-renewing cells with the potential to develop into cells of all three germ layers. See MorehPSCs are a valuable tool for researchers aiming to model human neurological development and disease. Generating iPS cells from patient samples provides the opportunity to obtain disease-specific neural stem cells, neurons, and glia.
The first step in your hPSC-to-neural workflow is neural induction of hPSCs to generate central nervous system neural progenitor cells (NPCs). To achieve reproducible and efficient neural induction, use the defined and serum-free STEMdiff™ SMADi Neural Induction Kit that mimics the in vivo neural developmental pathway. This kit is compatible with both embryoid body and monolayer neural induction protocols. The embryoid body protocol uses AggreWell™800 plates to form uniform neural aggregates, which, when replated are characterized by the presence of morphologically distinct neural rosette clusters that can be isolated for further experiments using STEMdiff™ Neural Rosette Selection Reagent.
Researchers performing neural induction can also generate neural crest cells (NCCs) to study a variety of pathologies and downstream mesenchymal cell types, or to differentiate peripheral nervous system cell types, including sensory neurons and Schwann cells. If hPSC-derived NCCs are your progenitor cell type of interest, you can obtain a highly pure population from hPSCs using the STEMdiff™ Neural Crest Differentiation Kit.
You can also generate organoids containing various differentiated neural cell types arranged in a 3D tissue structure from hPSCs. STEMdiff™ neural organoid kits enabling developmental self-organization or patterning to particular nervous system subregions are powerful tools to study human developmental processes and complex cell interactions.
- Tech Tip Designing Your Neural Induction and Differentiation Workflow
- Video An Embryoid Body-Based Procedure for Neural Induction of hPSCs Using STEMdiff™ Neural Induction Medium
- Manual STEMdiff™ Neural System Technical Manual
- Webinar Modeling Human Neurological Disease with Induced Pluripotent Stem Cells
- Tech Tip How to Choose Your Neural Organoid Model System
Cell Culture Media and Supplements (4 13)
Culture medium kit for establishment of organoid models containing human pluripotent stem cell-derived choroid plexus-like epithelium
Cell culture medium kit for robust generation of ventral forebrain neural organoids from human pluripotent stem cells
Cell culture medium kit for robust generation of dorsal forebrain neural organoids from human pluripotent stem cells
Cell culture kit for establishment of hPSC-derived neural crest cells
Cell Dyes and Detection Assay Kits (4 1)
Cell Storage Media (4 1)
For cryopreservation of neural progenitor cells generated using STEMdiff™ Neural Induction Medium
Cultureware and General Supplies (4 2)
Tissue and Cell Culture Dissociation Reagents (4 3)
Antibodies (4 1)
Mouse monoclonal IgG1 antibody against human, cynomolgus nestin
Cytokines and Proteins (4 2)
Small Molecules (4 10)
Buffers and Solutions (4 2)
Training and Education (4 1)
Training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors