Expansion of neural progenitor cells (NPCs) after neural induction generates a larger pool of cells for downstream applications. See MoreWhen conducting replicate or follow-up experiments, researchers are able to return to NPCs generated from the original neural induction procedure, removing a significant source of variability. STEMdiff™ Neural Progenitor Medium is optimized for the expansion of NPCs generated using STEMdiff™ Neural Induction Medium. While preserving multipotency, STEMdiff™ Neural Progenitor Medium minimizes spontaneous neuronal differentiation. NPCs can be frozen at any point post-neural induction with reproducibly high recovery rates with STEMdiff™ Neural Progenitor Freezing Medium. Post-thaw, NPCs display healthy morphology, express NPC markers, and retain the potential to expand and differentiate into neurons and glia.
As an alternative to neural induction, highly pure cryopreserved hPSC-derived NPCs are available from various cell lines. If you wish to expand these cells, use Neural Progenitor Medium 2, not STEMdiff™ Neural Progenitor Medium.
- Manual STEMdiff™ Neural System Technical Manual
- Webinar (1:05:46) Modeling Human Neurological Disease with Induced Pluripotent Stem Cells
- Scientific Poster Generation, Maintenance, and Cryopreservation of Neural Progenitor Cells Derived from Human Pluripotent Stem Cells Using the STEMdiff Neural System
Cell Culture Media and Supplements (45)
Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells
Serum-free neural supplement without insulin
Serum-free neural supplement without antioxidants
Culture medium kit for establishment and maturation of human cerebral organoids
Cell Dyes and Detection Assay Kits (42)
Cell Storage Media (41)
For cryopreservation of neural progenitor cells generated using STEMdiff™ Neural Induction Medium
Tissue and Cell Culture Dissociation Reagents (41)
Small Molecules (41)
Training and Education (41)
Hands-on training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors.