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STEMdiff™ Neural Progenitor Freezing Medium

For cryopreservation of neural progenitor cells generated using STEMdiff™ Neural Induction Medium

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STEMdiff™ Neural Progenitor Freezing Medium

For cryopreservation of neural progenitor cells generated using STEMdiff™ Neural Induction Medium

100 mL
Catalog #05838
224 USD

Overview

STEMdiff™ Neural Progenitor Freezing Medium is a defined and serum-free medium for cryopreservation of neural progenitor cells (NPCs) derived from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. This freezing medium is optimized for the cryopreservation of NPCs generated using STEMdiff™ Neural Induction Medium (Catalog #05835) and cultured in STEMdiff™ Neural Progenitor Medium (Catalog #05833). NPCs can be frozen at any point post-neural induction, with reproducibly high recovery rates. Post-thaw, NPCs display healthy morphology, express NPC markers, and retain the potential to expand and differentiate into neurons.
Advantages:
• Defined and serum-free
• Optimized for cryopreservation of NPCs, with reproducibly high recovery rates
• Supports cryopreservation of NPCs generated using STEMdiff™ Neural Induction Medium and cultured in STEMdiff™ Neural Progenitor
Medium
• Preserves NPC multipotency and expansion capacities
• Convenient, user-friendly format and protocol
Contains:
• Dimethyl sulfoxide (DMSO)
• Other ingredients
Cell Type:
Pluripotent Stem Cells; Neural Stem and Progenitor Cells; Neural Cells, PSC-Derived
Species:
Human
Brand:
STEMdiff
Area of Interest:
Neuroscience; Stem Cell Biology; Disease Modeling
Formulation:
Defined; Serum-Free

Scientific Resources

Educational Materials

(8)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Recovery of Neural Progenitor Cells Cryopreserved in STEMdiff™ Neural Progenitor Freezing Medium

Figure 1. Recovery of Neural Progenitor Cells Cryopreserved in STEMdiff™ Neural Progenitor Freezing Medium

NPCs cryopreserved in STEMdiff™ Neural Progenitor Freezing Medium (NPFM) show reproducibly high recovery after thawing. Recovery is comparable to cryopreservation in serum-containing medium (90% FBS / 10% DMSO). n = 3 independent experiments. Percent recovery defined as percentage of cells frozen that remain viable after thaw.

Neural Progenitor Cells Cryopreserved in STEMdiff™ Neural Progenitor Freezing Medium Retain Neural Progenitor Cell Properties

Figure 2. Neural Progenitor Cells Cryopreserved in STEMdiff™ Neural Progenitor Freezing Medium Retain Neural Progenitor Cell Properties

NPCs previously frozen in STEMdiff™ Neural Progenitor Freezing Medium display healthy morphology (A, one day after thaw), express NPC marker SOX1 (B, red) and can be differentiated into MAP2+ (C, red) and class III β-tubulin+ (C, green) neurons.

Publications

(1)
Stem Cell Reviews and Reports 2016 AUG

Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells

Robinson M et al.

Abstract

Pluripotent stem cells can become any cell type found in the body. Accordingly, one of the major challenges when working with pluripotent stem cells is producing a highly homogenous population of differentiated cells, which can then be used for downstream applications such as cell therapies or drug screening. The transcription factor Ascl1 plays a key role in neural development and previous work has shown that Ascl1 overexpression using viral vectors can reprogram fibroblasts directly into neurons. Here we report on how a recombinant version of the Ascl1 protein functionalized with intracellular protein delivery technology (Ascl1-IPTD) can be used to rapidly differentiate human induced pluripotent stem cells (hiPSCs) into neurons. We first evaluated a range of Ascl1-IPTD concentrations to determine the most effective amount for generating neurons from hiPSCs cultured in serum free media. Next, we looked at the frequency of Ascl1-IPTD supplementation in the media on differentiation and found that one time supplementation is sufficient enough to trigger the neural differentiation process. Ascl1-IPTD was efficiently taken up by the hiPSCs and enabled rapid differentiation into TUJ1-positive and NeuN-positive populations with neuronal morphology after 8 days. After 12 days of culture, hiPSC-derived neurons produced by Ascl1-IPTD treatment exhibited greater neurite length and higher numbers of branch points compared to neurons derived using a standard neural progenitor differentiation protocol. This work validates Ascl1-IPTD as a powerful tool for engineering neural tissue from pluripotent stem cells.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.