Human Pluripotent Stem Cell Research

Human embryonic stem (ES) and induced pluripotent stem (iPS) cells are increasingly being differentiated into specialized cell types for a wide range of applications, such as disease modeling, drug discovery, toxicity screening and cell therapy. See More While techniques have been developed to differentiate hPSCs into many adult cell types, many protocols are still inefficient and inconsistent. To reduce variability when differentiating hPSCs, use our specialized kits, reagents and user-friendly protocols to generate cells of the ectodermal , mesodermal and endodermal lineages. For flexible, user-directed differentiation, use cytokine-free specialized cell culture media such as , , TeSR™-E5 , or TeSR™-E6 to direct differentiation to your lineage of choice.

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Technical Bulletin STEMdiff™ APEL Medium
Tech Tip Control EB Size for Differentiation Experiments with AggreWell™
Video (2:53) STEMdiff™ Neural System for hPSC-Based Neurological Modeling
Wallchart Directed Differentiation of Pluripotent Stem Cells Wallchart

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Products

Cell Culture Media and Supplements 4 (75)

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STEMdiff™ Motor Neuron Maturation Kit

Cell culture medium kit for the maturation of hPSC-derived motor neurons
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STEMdiff™ Motor Neuron Differentiation Kit

Differentiation kit for the generation of motor neurons from hPSCs
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STEMdiff™ Blood Vessel Organoid Kit

Media and supplements for differentiation of human pluripotent stem cells to blood vessel organoids
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STEMdiff™ NK Cell Kit

For expansion and differentiation of hPSCs to NK cells

Small Molecules 4 (28)

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TTNPB

Retinoid pathway activator; Activates retinoic acid receptor (RAR)
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AICAR

AMPK activator
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Stauprimide

Inhibitor of NME2 localization; Suppresses c-MYC expression
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LY364947

Activin/BMP/TGF-β pathway inhibitor; Inhibits ALK5

Cytokines and Proteins 4 (18)

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Human Recombinant TGF-beta 1 (Cell Culture Ready)

Ready-to-use transforming growth factor beta 1
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Human Recombinant R-Spondin-1

R-Spondin-1
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Human Recombinant BMP-4

Bone morphogenetic protein 4
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Human Recombinant VEGF-165, ACF

Vascular endothelial growth factor, animal component-free

Matrices and Substrates 4 (6)

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0.1% Gelatin in Water

Coating for cultureware
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CellAdhere™ Type I Collagen, Human, Lyophilized

Type I collagen, human, for tissue engineering research and cell culture
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CellAdhere™ Tropoelastin, Human, Lyophilized

Purified tropoelastin, human, lyophilized, for tissue engineering research and cell culture
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CellAdhere™ Type I Collagen, Bovine, Solution

Purified bovine collagen for tissue engineering research, cell culture, and biochemistry

Tissue and Cell Culture Dissociation Reagents 4 (4)

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Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent
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STEMdiff™ Neural Rosette Selection Reagent

Enzyme-free reagent for the selective detachment of neural rosettes
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ACCUTASE™

Cell detachment solution
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ReLeSR™

cGMP, enzyme-free human pluripotent stem cell selection and passaging reagent

Cell Storage Media 4 (8)

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STEMdiff™ Cardiomyocyte Freezing Medium

Medium for cryopreserving hPSC-derived cardiomyocytes
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CryoStor® CS2

Animal component-free, defined cryopreservation medium with 2% DMSO
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STEMdiff™ Neural Progenitor Freezing Medium

For cryopreservation of neural progenitor cells generated using STEMdiff™ Neural Induction Medium
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FreSR™-S

Animal component-free medium for cryopreserving ES and iPS cells as single cells

Antibodies 4 (4)

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Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3

Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
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Anti-Beta-Tubulin III Antibody, Clone AA10

Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
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Anti-Human Nestin Antibody, Clone 10C2

Mouse monoclonal IgG1 antibody against human, cynomolgus nestin
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Anti-GFAP Antibody, Clone 2E1.E9

Mouse monoclonal IgG2b antibody against human, mouse, rat GFAP (glial fibrillary acidic protein)

Cultureware and General Supplies 4 (4)

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Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)
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AggreWell™400

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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AggreWell™800

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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Reversible Strainers

Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

Cell Isolation Products 4 (1)

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EasySep™ Human CD34 Positive Selection Kit II

Immunomagnetic positive selection kit

Buffers and Solutions 4 (5)

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Anti-Adherence Rinsing Solution

Rinsing solution for cultureware to prevent cell adhesion
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HBSS, Modified (Without Ca++ and Mg++)

Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium
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HBSS with 10 mM HEPES, Without Phenol Red

Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red
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RoboSep™ Buffer 2

Cell separation buffer

Cell Dyes and Detection Assay Kits 4 (6)

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GloCell™ Fixable Viability Dye Violet 540

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 510

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye UV 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

Training and Education 4 (1)

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Pluripotent Stem Cell Training

Training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors

Cell Engineering and Molecular Tools 4 (3)

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Human Pluripotent Stem Cell-Derived Endoderm qPCR Array

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages
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qPCR Master Mix Kit

For probe-based assays and arrays
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Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array

For characterization of hPSC trilineage differentiation to endoderm, mesoderm, and ectoderm germ layers

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