Human Pluripotent Stem Cell Research

Human embryonic stem (ES) and induced pluripotent stem (iPS) cells are increasingly being differentiated into specialized cell types for a wide range of applications, such as disease modeling, drug discovery, toxicity screening and cell therapy. See More While techniques have been developed to differentiate hPSCs into many adult cell types, many protocols are still inefficient and inconsistent. To reduce variability when differentiating hPSCs, use our specialized kits, reagents and user-friendly protocols to generate cells of the ectodermal, mesodermal and endodermal lineages. For flexible, user-directed differentiation, use cytokine-free specialized cell culture media such as , , TeSR™-E5, or TeSR™-E6 to direct differentiation to your lineage of choice.

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Technical Bulletin STEMdiff™ APEL Medium
Tech Tip Control EB Size for Differentiation Experiments with AggreWell™
Video (2:53) STEMdiff™ Neural System for hPSC-Based Neurological Modeling
Wallchart Directed Differentiation of Pluripotent Stem Cells Wallchart

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Products

Cell Culture Media and Supplements (432)

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STEMdiff™ Hematopoietic Kit

For differentiation of human ES or iPS cells into hematopoietic progenitor cells
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BrainPhys™ Without Phenol Red

Serum-free neurophysiological basal medium for improved neuronal function
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STEMdiff™ Cerebral Organoid Kit

Culture medium kit for establishment and maturation of human cerebral organoids
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STEMdiff™ Neural Induction Medium

Defined, serum-free medium for neural induction of human ES and iPS cells

Small Molecules (426)

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5-Azacytidine

Epigenetic modifier; Inhibits DNA methyltransferase (DNMT)
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Sodium Butyrate

Epigenetic modifier; Inhibits histone deacetylase
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SB202190

p38 MAPK inhibitor
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Stauprimide

Inhibitor of NME2 localization; Suppresses c-MYC expression

Cytokines (418)

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Human Recombinant Flt3/Flk-2 Ligand

Fms-like tyrosine kinase 3/fetal liver kinase-2
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Human Recombinant LIF

Leukemia inhibitory factor
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Human Recombinant Noggin

Noggin
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Human Recombinant SCF

Stem cell factor

Matrices and Substrates (45)

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Vitronectin XF™

Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
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CellAdhere™ Type I Collagen, Bovine, Solution

Purified bovine collagen for tissue engineering research, cell culture, and biochemistry
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CellAdhere™ Tropoelastin, Human, Lyophilized

Purified tropoelastin, human, lyophilized, for tissue engineering research and cell culture
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CellAdhere™ Type I Collagen, Human, Lyophilized

Type I collagen, human, for tissue engineering research and cell culture

Tissue and Cell Culture Dissociation Reagents (44)

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STEMdiff™ Neural Rosette Selection Reagent

Enzyme-free reagent for the selective detachment of neural rosettes
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ReLeSR™

Enzyme-free human ES and iPS cell selection and passaging reagent
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Gentle Cell Dissociation Reagent

Enzyme-free cell dissociation reagent
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ACCUTASE™

Cell detachment solution

Cell Storage Media (46)

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mFreSR™

Defined, serum-free cryopreservation medium for human ES and iPS cells
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STEMdiff™ Neural Progenitor Freezing Medium

For cryopreservation of neural progenitor cells generated using STEMdiff™ Neural Induction Medium
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CryoStor® CS2

Animal component-free, defined cryopreservation medium with 2% DMSO
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CryoStor® CS5

Animal component-free, defined cryopreservation medium with 5% DMSO

Antibodies (45)

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Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3

Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
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Anti-Beta-Tubulin III Antibody, Clone AA10

Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
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STEMdiff™ Human Neural Progenitor Antibody Panel

Antibody panel for the identification of neural progenitor cells and undifferentiated human ES/iPS cells
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Anti-Human Nestin Antibody, Clone 10C2

Mouse monoclonal IgG1 antibody against human, cynomolgus nestin

Cultureware and General Supplies (45)

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AggreWell™800

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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AggreWell™400

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)
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10 mL Tubes

10 mL tubes, 100 pack

Cell Isolation Products (41)

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EasySep™ Human CD34 Positive Selection Kit II

Immunomagnetic positive selection kit

Buffers and Solutions (45)

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RoboSep™ Buffer 2

Cell separation buffer
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Anti-Adherence Rinsing Solution

Rinsing solution for cultureware to prevent cell adhesion
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HBSS with 10 mM HEPES, Without Phenol Red

Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red
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HBSS, Modified (Without Ca++ and Mg++)

Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium

Primary and Cultured Cells (415)

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Human PSC-Derived Neural Progenitor Cells

Cryopreserved human PSC-derived neural progenitor cells for expansion and differentiation
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Human PSC-Derived Mixed Neurons

Cryopreserved human PSC-derived neuronal precursor cells for maturation into forebrain-type neurons
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Human PSC-Derived Dopaminergic Neurons

Cryopreserved human PSC-derived dopaminergic neuron precursor cells for maturation into midbrain-type dopaminergic neurons
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Human PSC-Derived Astrocytes

Cryopreserved human PSC-derived astrocyte precursor cells for maturation into astrocytes

Genome Editing and Molecular Tools (43)

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Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array

For characterization of hPSC trilineage differentiation to endoderm, mesoderm, and ectoderm germ layers
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qPCR Master Mix Kit

For probe-based assays and arrays
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Human Pluripotent Stem Cell-Derived Endoderm qPCR Array

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages

Training and Education (41)

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Pluripotent Stem Cell Training

Hands-on training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors.

Cell Dyes and Detection Assay Kits (46)

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GloCell™ Fixable Viability Dye Red 710

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Red 780

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye UV 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells