Human Pluripotent Stem Cell Research

Human embryonic stem (ES) and induced pluripotent stem (iPS) cells are increasingly being differentiated into specialized cell types for a wide range of applications, such as disease modeling, drug discovery, toxicity screening and cell therapy. See More While techniques have been developed to differentiate hPSCs into many adult cell types, many protocols are still inefficient and inconsistent. To reduce variability when differentiating hPSCs, use our specialized kits, reagents and user-friendly protocols to generate cells of the ectodermal , mesodermal and endodermal lineages. For flexible, user-directed differentiation, use cytokine-free specialized cell culture media such as , , TeSR™-E5 , or TeSR™-E6 to direct differentiation to your lineage of choice.

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Technical Bulletin STEMdiff™ APEL Medium
Tech Tip Control EB Size for Differentiation Experiments with AggreWell™
Video (2:53) STEMdiff™ Neural System for hPSC-Based Neurological Modeling
Wallchart Directed Differentiation of Pluripotent Stem Cells Wallchart

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Products

Cell Culture Media and Supplements (4 61)

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STEMdiff™ Blood Vessel Organoid Kit

Media and supplements for differentiation of human pluripotent stem cells to blood vessel organoids
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STEMdiff™ Sensory Neuron Maturation Kit

Maturation kit for generation of functional sensory neurons from human iPS cell-derived sensory neuronal precursor cells
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STEMdiff™ Sensory Neuron Differentiation Kit

Differentiation kit for the generation of sensory neuronal precursors from human iPS cell-derived neural crest cells
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STEMdiff™ Choroid Plexus Organoid Maturation Kit

Culture medium kit for establishment of organoid models containing human pluripotent stem cell-derived choroid plexus-like epithelium

Small Molecules (4 26)

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SB431542

Activin/BMP/TGF-β pathway inhibitor; Inhibits ALK4, ALK5, and ALK7
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Dorsomorphin

BMP and AMPK pathway inhibitor; Inhibits ALK2, ALK3, ALK6, and AMPK
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LDN193189

BMP pathway inhibitor; Inhibits ALK1, ALK2, ALK3, and ALK6
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CHIR98014

WNT pathway activator; Inhibits GSK3α and GSK3ß

Cytokines and Proteins (4 18)

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Human Recombinant TGF-beta 1 (Cell Culture Ready)

Ready-to-use transforming growth factor beta 1
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Human Recombinant R-Spondin-1

R-Spondin-1
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Human Recombinant BMP-4

Bone morphogenetic protein 4
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Human Recombinant VEGF-165, ACF

Vascular endothelial growth factor, animal component-free

Matrices and Substrates (4 5)

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Vitronectin XF™

Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
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CellAdhere™ Type I Collagen, Bovine, Solution

Purified bovine collagen for tissue engineering research, cell culture, and biochemistry
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CellAdhere™ Tropoelastin, Human, Lyophilized

Purified tropoelastin, human, lyophilized, for tissue engineering research and cell culture
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CellAdhere™ Type I Collagen, Human, Lyophilized

Type I collagen, human, for tissue engineering research and cell culture

Tissue and Cell Culture Dissociation Reagents (4 4)

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ACCUTASE™

Cell detachment solution
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STEMdiff™ Neural Rosette Selection Reagent

Enzyme-free reagent for the selective detachment of neural rosettes
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ReLeSR™

cGMP, enzyme-free human pluripotent stem cell selection and passaging reagent
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Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent

Cell Storage Media (4 8)

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CryoStor® CS5

Animal component-free, defined cryopreservation medium with 5% DMSO
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CryoStor® CSB

Animal component-free and protein-free cryopreservation formulation
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STEMdiff™ Cardiomyocyte Freezing Medium

Medium for cryopreserving hPSC-derived cardiomyocytes
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mFreSR™

Serum-free cryopreservation medium for human ES and iPS cells

Antibodies (4 4)

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Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3

Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
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Anti-Beta-Tubulin III Antibody, Clone AA10

Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
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Anti-Human Nestin Antibody, Clone 10C2

Mouse monoclonal IgG1 antibody against human, cynomolgus nestin
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Anti-GFAP Antibody, Clone 2E1.E9

Mouse monoclonal IgG2b antibody against human, mouse, rat GFAP (glial fibrillary acidic protein)

Cultureware and General Supplies (4 4)

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Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)
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AggreWell™400

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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AggreWell™800

Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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Reversible Strainers

Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

Cell Isolation Products (4 1)

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EasySep™ Human CD34 Positive Selection Kit II

Immunomagnetic positive selection kit

Buffers and Solutions (4 5)

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RoboSep™ Buffer 2

Cell separation buffer
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Anti-Adherence Rinsing Solution

Rinsing solution for cultureware to prevent cell adhesion
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HBSS with 10 mM HEPES, Without Phenol Red

Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red
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HBSS, Modified (Without Ca++ and Mg++)

Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium

Cell Dyes and Detection Assay Kits (4 6)

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GloCell™ Fixable Viability Dye Violet 540

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 510

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye UV 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

Training and Education (4 1)

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Pluripotent Stem Cell Training

Training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors

Genome Editing and Molecular Tools (4 3)

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Human Pluripotent Stem Cell-Derived Endoderm qPCR Array

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages
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qPCR Master Mix Kit

For probe-based assays and arrays
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Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array

For characterization of hPSC trilineage differentiation to endoderm, mesoderm, and ectoderm germ layers

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