Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array

For characterization of hPSC trilineage differentiation to endoderm, mesoderm, and ectoderm germ layers
Catalog #
07511_C
For characterization of hPSC trilineage differentiation to endoderm, mesoderm, and ectoderm germ layers
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Required Products
  1. qPCR Master Mix Kit|07516
    qPCR Master Mix Kit

    For probe-based assays and arrays

  2. Axygen® Sealing Film for Real-Time PCR (qPCR)
    Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

    Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)

  3. Total RNA Purification Kit
    Total RNA Purification Kit

    For purification of RNA from cells

  4.  STEMscript™ cDNA Synthesis Kits|79003
    STEMscript™ cDNA Synthesis Kits

    For reverse transcription of RNA before qPCR or RNA sequencing

Overview

The Human Pluripotent Stem Cell (hPSC) Trilineage Differentiation Quantitative Polymerase Chain Reaction (qPCR) Array is designed for characterization of hPSCs and their trilineage differentiation capacity. hPSCs, including embryonic stem (ES) and induced pluripotent stem (iPS) cells, are self-renewing and have the ability to differentiate into cells of the three embryonic germ layers: ectoderm, mesoderm, and endoderm. This qPCR array provides the gene expression profile of undifferentiated ES and iPS cells and their trilineage derivatives following in vitro directed or spontaneous differentiation, thereby validating the ability of a cell line to differentiate to the three germ layers. Genes were selected based on their demonstrated differential expression in ES and iPS cells compared with hPSC-derived ectodermal, mesodermal, and endodermal lineage cells (Adewumi et al.; Bock et al.).

qPCR is used to determine changes in steady-state mRNA levels of gene expression across multiple samples, generally normalized to the relative expression of internal control genes. Gene-specific primers amplify target sequences within cDNA pools reverse-transcribed from mRNA and, as with TaqMan® technology, hybridized sequence-specific probes provide a fluorescent signal from the 5' exonuclease activity of Taq DNA polymerase. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, and thereby the amount of mRNA in the original cell lysate.

This qPCR array contains validated primers and probes for detection of 90 genes whose expression is correlated with undifferentiated hPSCs or their derivatives undergoing the early stages of differentiation, as well as six endogenous (housekeeping) control genes. TBP (TATA box-binding protein) qPCR Control Template is provided as a synthetic DNA positive control. Additional gene information and a qPCR analysis tool are available at www.stemcell.com/qPCRanalysis
Components
  • Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array (Catalog #07515)
    • hPSC Trilineage qPCR Array, 1 x 384-well plate
    • TBP qPCR Control Template, 1 vial
Cell Type
Endoderm, PSC-Derived, Mesoderm, PSC-Derived, Neural Cells, PSC-Derived, Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array
Catalog #
07515
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array
Catalog #
07515
Lot #
All
Language
English

Educational Materials(4)

Brochure
qPCR Arrays for Cell Characterization
Video
8:33
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
Webinar
55:52
Best Practice Criteria for Pluripotent Stem Cell Lines
Webinar
17:46
Nature Research Round Table: Pluripotency Tests

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array Plate Configuration

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