Anti-Human CD56 (NCAM) Antibody, Clone HCD56
Mouse monoclonal IgG1 antibody against human CD56 (NCAM)
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Overview
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA) and EasySep™ Human CD56 Positive Selection Kit (Catalog #18055); partial blocking may be observed, as well as EasySep™ HLA CD2 Positive Selection Kit (Catalog #18657HLA) and EasySep™ Human NK Cell Enrichment Kit (Catalog #19055).
Data Figures
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC and Anti-Human CD45 Antibody, Clone HI30, APC.
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD56 Positive Selection Kit and labeled with Anti-Human CD56 Clone HCD56, Alexa Fluor® 488. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).
Figure 3. Data for APC-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, PE. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).
Figure 4. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, Biotin, followed by streptavidin (SAV) APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC and Anti-Human CD45 Antibody, Clone HI30, PE. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, Biotin followed by SAV APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC is shown (solid line histogram).
Figure 5. Data for FITC-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, FITC and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa FITC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).
Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD56 Antibody, Clone HCD56, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
Related Products
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
