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The HCD56 antibody reacts with CD56 (NCAM), a member of the immunoglobulin (Ig) super family containing 5 Ig-like domains and two fibronectin type-3 domains in its extracellular portion. Multiple isoforms have been identified, including transmembrane and GPI-anchored forms. CD56 is subject to several types of post-translational modification, including addition of a polysialic acid moiety to the 5th Ig-like domain. The ~140 kDa isoform of CD56 is considered the prototypic cell surface marker on human NK cells. Around 90% of the NK cells express CD56 at low density while ~10% express the protein at relatively high levels. CD56 is also variably expressed by a subset of NK-T cells in peripheral blood, neurons, muscle cells, some stem cells, and in vitro-cultured T cell clones. It is also expressed by several types of tumors, including myeloma, neuroblastoma and small cell lung carcinoma cells. CD56 mediates cell-cell and cell-matrix adhesion via homophilic and heterophilic interactions, respectively, and has been implicated in activating intracellular signaling pathways. CD56 plays important roles in neuronal development both during embryogenesis and in adults.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA) and EasySep™ Human CD56 Positive Selection Kit (Catalog #18055); partial blocking may be observed, as well as EasySep™ HLA CD2 Positive Selection Kit (Catalog #18657HLA) and EasySep™ Human NK Cell Enrichment Kit (Catalog #19055).
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).
(B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC and Anti-Human CD45 Antibody, Clone HI30, APC.
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD56 Positive Selection Kit and labeled with Anti-Human CD56 Clone HCD56, Alexa Fluor® 488. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).
Figure 3. Data for APC-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE).
(B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, PE.
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).
Figure 4. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, Biotin, followed by streptavidin (SAV) APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE).
(B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC and Anti-Human CD45 Antibody, Clone HI30, PE.
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, Biotin followed by SAV APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC is shown (solid line histogram).
Figure 5. Data for FITC-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD56 Antibody, Clone HCD56, FITC and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).
(B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa FITC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC.
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Buffy Coat CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).
Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD56 Antibody, Clone HCD56, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD56 Positive Selection Kit and labeled with Anti-Human CD56 Antibody, Clone HCD56, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
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Mouse monoclonal IgG1, kappa isotype control antibody
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Anti-Human CD56 Antibody, Clone HCD56
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
