HLA Analysis

Cell isolation followed by downstream cell analysis are crucial steps involved in HLA analysis. See MoreEnsure consistent downstream cell analysis, including purity assessments, by using high-quality antibodies.

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Technical Bulletin Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Tech Tip Considerations for FACS gating

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Products

Antibodies (4 16)

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Anti-Human CD8a Antibody, Clone SK1

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a
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Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2

Rat monoclonal IgG2b antibody against mouse MHC Class II (I-A/I-E)
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Anti-Human CD3 Antibody, Clone UCHT1

Mouse (BALB/c) monoclonal IgG1 antibody against human, chimpanzee CD3
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Anti-Human CD2 Antibody, Clone RPA-2.10

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2

Cell Dyes and Detection Assay Kits (4 8)

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GloCell™ Fixable Viability Dye Violet 450

Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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Trypan Blue

Reagent for counting viable mammalian cells
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3% Acetic Acid with Methylene Blue

Reagent for manual counting of nucleated mammalian cells
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GloCell™ Fixable Viability Dye Violet 540

Amine-labeling fluorescent dye for live/dead staining of mammalian cells

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