EasySep™ Cell Isolation & Cell Separation Technology

Scientific research can be demanding, so working efficiently is essential. You need the right tools that not only save time but also make your workflow easier. That’s why our scientists created EasySep™, a simpler, easier way to isolate the cells you need. This versatile technology combines the specificity of monoclonal antibodies with a column-free magnetic system, allowing you to get highly purified cells in as little as 8 minutes. Whether you're new to cell separation or an experienced researcher, choose EasySep™ to simplify your workflow—so you can focus on achieving great science.

EasySep™ Magnets

Ready to Simplify Your Workflow?

Curious about how EasySep™ can simplify your research? Experience how easy it is by requesting a sample. Say goodbye to complicated protocols and time-consuming, column-based cell isolations. Make the easy choice and move your research forward faster.

Why Use EasySep™ to Isolate Cells?

  • Isolate cells in as little as 8 minutes with a simple pour.
  • Achieve up to 99% cell purities with high recoveries.
  • Obtain viable, functional cells without the need for columns and washes.
  • Isolate cells from virtually any sample source, including whole blood and leukopaks.
  • Widely used in published research for over 20 years, supporting diverse downstream applications.

Explore the benefits of EasySep™, backed by performance and competitor data, to see the advantages for yourself.


How Does EasySep™ Magnetic Cell Separation Work?

Cells are targeted for either removal (negative selection and depletion) or positive selection using antibody complexes directed to specific cell surface antigens. The antibody complexes link targeted cells to EasySep™ magnetic particles. Labeled cells are pulled to the sides of the tube when the sample is placed in an EasySep™ cell separation magnet, while untouched cells can be simply poured or pipetted off into a new tube. EasySep™ methods are column-free, reducing mechanical stress on cells and preserving their functionality and viability.

Figure 1. Comparison of Two Different Immunomagnetic Cell Isolation Protocols

Steps involved in the isolation of human T cells by negative selection using two different immunomagnetic cell isolation technologies are shown: (A) column-free EasySep™ technology (time taken may vary depending on the type of magnet used) and (B) a commercially available column-based technology (*elution time varies).


Immunomagnetic-based EasySep™ technology supports cell isolation from a wide range of species (e.g. human, mouse, non-human primate, and rat) and sample sources (e.g. peripheral blood mononuclear cells (PBMCs), leukopaks, whole blood, splenocytes, lymph nodes, cord blood, and bone marrow).* Learn more about how EasySep™ magnetic cell separation technology works and key factors to consider when choosing a negative or positive selection strategy for your research needs.


EasySep™–A Trusted Cell Isolation Method for Over 20 Years

EasySep™ has been extensively referenced in more than 6,500 peer-reviewed studies for over 20 years, demonstrating consistent performance across various cell types and downstream applications, including flow cytometry, cell culture, cell expansion, DNA/RNA extraction, sequencing, cell-based assays, and more. The effectiveness and versatility of EasySep™ technology has led to widespread adoption in fields of study such as immunology, cancer, stem cell biology, and cell therapy research. Browse our Immunology Features highlighting major scientific advances supported by EasySep™ products and explore select publications showcasing how cells isolated with EasySep™ are used.

We use EasySep™ because of its ease of use and the high purity that we get from the preparation. I especially like the negative selection kits because it allows us to keep the cells that we collect as undisturbed as possible.

Eric, MD/PhD Student

They are very efficient kits and I can perform my isolations quickly without compromising on cell quality or viability.

Payel, Postdoctoral Fellow

I currently use the EasySep™ Mouse T Cell Isolation Kit, CD4+ T Cell Isolation Kit, and CD8+ T Cell Isolation Kit. I have used different isolation kits over the years and these yield superior results, in my opinion.

Cori, PhD Student

Thousands of labs worldwide already trust EasySep™ for its speed and simplicity in isolating highly purified cells. Transitioning to EasySep™ for your cell isolation needs is easier than you think. Get started now by requesting a sample or hear more from EasySep™ users about their research.


EasySep™ Frequently Asked Questions (FAQs) and Product Selection Resources

Find quick answers to frequently asked questions (FAQs) about EasySep™ cell isolation, product compatibility, sample requests, and more. Alternatively, explore the information below to help you find the right EasySep™ product for your application.

Looking for additional EasySep™ technology and protocol guidance to get the best results in your experiments? Explore our comprehensive range of EasySep™ technical tips, or browse our Methods Library for more resources and cell separation protocols by negative selection and positive selection. Alternatively, please contact us at techsupport@stemcell.com for assistance.

Explore EasySep™ Products for Human, Mouse, and Other Species

Explore a wide range of EasySep™ products organized by species and application, making it easier than ever to find the right isolation kit for your research. Don’t see a product for your application? Custom cell separation kits can be tailored to isolate specific or rare cell types not addressed by standard products. For more information or to discuss your specific cell isolation requirements, please contact your local sales representative or the Product & Scientific Support team at techsupport@stemcell.com.


EasySep™ Human Cell Separation Kits

EasySep™ Human Cell Isolation Kits

EasySep™ Human Cell Isolation Kits

Typical Sample Source:

Protocol Time:

As Little As 8 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • Easy to use
  • High purity (up to 98%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™

EasySep™ Human Positive Selection Kits

EasySep™ Human Positive Selection Kits

Typical Sample Source:

Protocol Time:

As Little As 15 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • Easy to use
  • High purity (up to 99%)
  • Column-free
  • Can be fully automated using RoboSep™

EasySep™ Direct Cell Isolation Kits

EasySep™ Direct

Typical Sample Source:

Protocol Time:

As Little As 20 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • Easy to use
  • 99.9% RBC depletion without density gradient centrifugation, lysis or sedimentation
  • High purity (up to 99%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis

EasySep™ Release Cell Separation Kits

EasySep™ Release

Typical Sample Source:

Protocol Time:

As Little As 29 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • Easy to use
  • High purity (up to 99%)
  • Column-free
  • Magnetic particle-free cells
  • Flexibility to perform sequential positive selections to isolate unique and rare cell types
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*Certain products are only available in select territories. Please contact your local sales representative or the Product & Scientific Support team at techsupport@stemcell.com for further information.


EasySep™ Mouse and Rat Cell Separation Kits

EasySep™ Mouse Cell Isolation Kits

Typical Sample Source:

Spleen, Bone Marrow, Whole Blood, Lymph Nodes

Protocol Time:

As Little As 15 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • Easy and fast
  • High purity (up to 98%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™

EasySep™ Mouse Positive Selection Kits

Typical Sample Source:

Spleen, Bone Marrow, Whole Blood, Lymph Nodes

Protocol Time:

As Little As 15 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • Easy and fast
  • High purity (up to 98%)
  • Column-free
  • Can be fully automated using RoboSep™

EasySep™ Rat Cell Isolation Kits

Typical Sample Source:

Spleen, Lymph Nodes, Whole Blood

Protocol Time:

As Little As 13 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • Easy and fast
  • High purity (up to 98%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™
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How to Choose the Right EasySep™ Magnet

EasySep™ Magnets

EasySep™ magnets are specifically designed for cell separation using EasySep™ reagents, eliminating the need for columns. They generate a high-gradient magnetic field within the interior cavity, effectively separating cells labeled with EasySep™ Magnetic Particles. EasySep™ magnets are available in a range of different sizes, allowing researchers to process a variety of sample volumes and cell numbers to suit their downstream application needs. Use this comparison table to help you choose the right magnet for your needs.

Automated Cell Separation with RoboSep™

RoboSep Cell Separation Instruments

EasySep™ immunomagnetic isolation can also be automated using RoboSep™ instruments, offering true walk-away automation options for labs requiring higher throughput, providing the same ease and reliability on a larger scale. Minimize sample handling and perform simultaneous and sequential cell isolations from multiple samples using RoboSep™-S and RoboSep™-16 instruments.

Learn More >

On-Demand Human Immune Cell Isolation Course

Human Immune Course

Learn how to effectively process human samples and isolate highly pure target cells with EasySep™ in this free self-paced course.

View Course >


Get Started with EasySep™ Today

Are you ready to make cell separation easy? Whether you’re ready to buy, want to request a free sample, or need a demo, we’re here to help simplify your research journey. Choose Your Next Steps:

Start achieving great science, the easy way.


Related Resources

*Certain products are only available in select territories. Please contact your local sales representative or the Product & Scientific Support team at techsupport@stemcell.com for further information.

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