EasySep™ Cell Isolation & Cell Separation Technology
EasySep™ Cell Separation
A Smarter, More Efficient Way to Isolate Cells
Working efficiently is key to overcoming the demands of scientific research. That’s why our scientists developed a smarter, more efficient way to isolate cells. EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system—for fast and easy isolation of highly purified cells that are ready for downstream applications. This versatile immunomagnetic cell separation technology enables positive selection, negative selection, or cell depletion.
EasySep™ can be used to isolate cells in as little as 8 minutes from a variety of species (e.g. human, mouse, non-human primate, rat, rabbit, cow and pig) and sample sources (e.g. splenocytes, lymph nodes, PBMCs, whole blood, leukopaks, cord blood, and bone marrow).*
View All EasySep™ Cell Separation Kits >
How Does EasySep™ Cell Separation Work?
Cells are targeted for either removal (negative selection and depletion) or positive selection using antibody complexes directed to specific cell surface antigens. The antibody complexes link targeted cells to EasySep™ magnetic particles. Labeled cells are pulled to the sides of the tube when the sample is placed in an EasySep™ cell separation magnet, while untouched cells can be simply poured or pipetted off into a new tube.
Why Use EasySep™ to Isolate Cells?
- Isolate cells in as little as 8 minutes with a simple pour.
- Achieve up to 99% cell purities with high recoveries.
- Obtain viable, functional cells without the need for columns and washes.
- Isolate cells from virtually any sample source, including whole blood and Leukopaks.
Is EasySep™ a Well-Accepted Cell Isolation Method?
Since its launch in 2002, EasySep™ has been used in thousands of labs worldwide to quickly and easily isolate highly purified cells for a wide variety of applications. More immunologists are making the switch to EasySep™ and freeing up time to accomplish more in the lab. Switching your cell isolation technology to EasySep™ is easier than you think.
We use EasySep™ because of its ease of use and the high purity that we get from the preparation. I especially like the negative selection kits because it allows us to keep the cells that we collect as undisturbed as possible
EasySep™ Human Cell Separation Kits
EasySep™ Human Cell Isolation Kits
Protocol Time:
Selection Method:
Features/Advantages:
- High purity (up to 98%)
- Column-free
- Untouched cells immediately ready for downstream analysis
- Can be fully automated using
EasySep™ Human Positive Selection Kits
Protocol Time:
Selection Method:
Features/Advantages:
- High purity (up to 99%)
- Column-free
- Can be fully automated using
EasySep™ Direct Cell Isolation Kits
Typical Sample Source:
Protocol Time:
Selection Method:
Features/Advantages:
- 99.9% RBC depletion without density gradient centrifugation, lysis or sedimentation
- High purity (up to 99%)
- Column-free
- Untouched cells immediately ready for downstream analysis
EasySep™ Release Cell Separation Kits
Protocol Time:
Selection Method:
Features/Advantages:
- High purity (up to 99%)
- Column-free
- Magnetic particle-free cells
- Flexibility to perform sequential positive selections to isolate unique and rare cell types
*Certain products are only available in select territories. Please contact your local Sales representative or the Product & Scientific Support team at techsupport@stemcell.com for further information.
They are very efficient kits and I can perform my isolations quickly without compromising on cell quality or viability.
On-Demand Human Immune Cell Isolation Course
Learn how to effectively process human samples and isolate highly pure target cells with EasySep™ in this free self-paced course.
View Course >EasySep™ Mouse and Rat Cell Separation Kits
View All EasySep™ Products for Rat Cell Separation Kits
EasySep™ Mouse Cell Isolation Kits
Typical Sample Source:
Protocol Time:
Selection Method:
Features/Advantages:
- Fast and easy
- High purity (up to 98%)
- Column-free
- Untouched cells immediately ready for downstream analysis
- Can be fully automated using
EasySep™ Mouse Positive Selection Kits
Typical Sample Source:
Protocol Time:
Selection Method:
Features/Advantages:
- Fast and easy
- High purity (up to 99%)
- Column-free
- Can be fully automated using
EasySep™ Rat Cell Isolation Kits
Typical Sample Source:
Protocol Time:
Selection Method:
Features/Advantages:
- Fast and easy
- High purity (up to 99%)
- Column-free
- Untouched cells immediately ready for downstream analysis
- Can be fully automated using
I currently use the EasySep™ Mouse T Cell Isolation Kit, CD4+ T Cell Isolation Kit, and CD8+ T Cell Isolation Kit. I have used different isolation kits over the years and these yield superior results, in my opinion.
EasySep™ for Other Species and Cell Types
EasySep™ cell separation technology can also be used to isolate cells from a variety of other species, including non-human primate, rabbit, cow and pig. If you are working with specific rare cell types, non-standard species, or special applications, you can explore our custom cell isolation solutions or contact us at info@stemcell.com to discuss your specific cell separation needs.Automated Cell Separation
EasySep™ cell separation technology can be automated using RoboSep™. Minimize sample handling and perform simultaneous and sequential cell isolations from multiple samples using RoboSep™-S and RoboSep™-16 instruments.
Related Resources
Magnetic Cell Separation
Find answers to three commonly asked questions about immunomagnetic cell separation.
Choosing a Positive or Negative Selection Approach
Read more about antibody labeling-based cell separation methods, including immunomagnetic and immunodensity cell separation.
Find the Right Antibody for Your Applications
Use our Antibody Finder Tool to select high-quality primary and secondary antibodies verified to work with EasySep™ products in specific applications.
Explore our cell separation methods library and immunology resource center for additional useful materials, including protocols, technical bulletins, and videos.
