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EasySep™

A Smarter, More Efficient Way to Isolate Cells

Working efficiently is key to overcome the demands of scientific research. That’s why our scientists have developed a smarter, more efficient way to isolate cells. EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system for fast and easy isolation of highly purified cells that are ready for downstream applications. This versatile immunomagnetic cell separation technology enables positive selection, negative selection, or cell depletion.

EasySep™ can be used to isolate cells in as little as 8 minutes from a variety of species (ex. human, mouse, non-human primate, rat, rabbit, cow and pig) and sample sources (ex. splenocytes, lymph nodes, PBMCs, whole blood, cord blood, bone marrow and leukapheresis samples).

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Fast and Easy Cell Isolation with EasySep™

How Does EasySep™ Cell Separation Work?

Cells are targeted for either removal (negative selection and depletion) or positive selection using antibody complexes directed to specific cell surface antigens. The antibody complexes link targeted cells to EasySep™ magnetic particles. Labeled cells are pulled to the sides of the tube when the sample is placed in an EasySep™ cell separation magnet, while untouched cells can be simply poured or pipetted off into a new tube.

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How Does EasySep™ Enable Research?

Is EasySep™ a Well-Accepted Cell Separation Method?

Since its launch in 2002, EasySep™ has been used by thousands of labs worldwide to quickly and easily isolate highly purified cells for a wide variety of applications. More immunologists are making the switch to EasySep™ so they can accomplish more with their time in the lab.

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Why Use EasySep™ to Isolate Cells?

  • Isolate cells in as little as 8 minutes with a simple pour.
  • Achieve up to 99% cell purities with high recoveries.
  • Obtain viable, functional cells without the need for columns and washes.
  • Isolate cells from virtually any sample source, including whole blood and Leuko Paks.

Switching your cell separation technology to EasySep™ is easier than you think.
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Already an EasySep™ user? Explore our next-generation EasySep™ kits and make sure your lab is using the most up-to-date kits.

EasySep™ for Human Cell Separation

EasySep™ Human Cell Isolation Kits

EasySep™ Human Cell Isolation Kits

Typical Sample Source:

PBMCs, Leuko Paks

Protocol Time:

As Little As 8 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • High purity (up to 98%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™

EasySep™ Human Positive Selection Kits

EasySep™ Human Positive Selection Kits

Typical Sample Source:

PBMCs, Leuko Paks

Protocol Time:

As Little As 15 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • High purity (up to 99%)
  • Column-free
  • Can be fully automated using RoboSep™

EasySep™ Direct Cell Isolation Kits

EasySep™ Direct

Typical Sample Source:

Whole Blood

Protocol Time:

As Little As 20 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • 99.9% RBC depletion without density gradient centrifugation, lysis or sedimentation
  • High purity (up to 99%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis

EasySep™ Release Cell Separation Kits

EasySep™ Release

Typical Sample Source:

PBMCs, Leuko Paks

Protocol Time:

As Little As 29 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • High purity (up to 99%)
  • Column-free
  • Magnetic particle-free cells
  • Flexibility to perform sequential positive selections to isolate unique and rare cell types
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EasySep™ for Mouse and Rat Cell Separation

EasySep™ Mouse Cell Isolation Kits

Typical Sample Source:

Spleen, Bone Marrow, Whole Blood, Lymph Nodes

Protocol Time:

As Little As 15 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • Fast and easy
  • High purity (up to 98%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™

EasySep™ Mouse Positive Selection Kits

Typical Sample Source:

Spleen, Bone Marrow, Whole Blood, Lymph Nodes

Protocol Time:

As Little As 15 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • Fast and easy
  • High purity (up to 99%)
  • Column-free
  • Can be fully automated using RoboSep™

EasySep™ Rat Cell Isolation Kits

Typical Sample Source:

Spleen, Lymph Nodes, Whole Blood

Protocol Time:

As Little As 13 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • Fast and easy
  • High purity (up to 99%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™
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EasySep™ for Other Species

EasySep™ cell separation technology can also be used to isolate cells from a variety of other species, including non-human primate, rabbit, cow and pig.
Copyright © 2018 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, AGGREWELL, CELLADHERE, CLONACELL, EASYPLATE, EASYSEP, ENDOCULT, EPICULT, ESCULT, ERYTHROCLEAR, FRESR, MAMMOCULT, MEGACULT, MESENCULT, METHOCULT, MYELOCULT, NEUROCULT, PNEUMACULT, PROSTACULT, RELESR, ROBOSEP, ROSETTESEP, SEPMATE, SMARTDISH, SPINSEP, STEMDIFF, STEMSEP, STEMSPAN, and STEMVISION are trademarks of STEMCELL Technologies Canada Inc. All other trademarks and registered trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.
 

How does EasySep™ Perform?

High Purity and Recovery

EasySep™ protocols are optimized for the efficient isolation of mouse cells with high purity and recovery.


Figure 1. EasySep™ Yields Equivalent or Better Purity and Recovery of Human T Cells Compared to a Column-Based Technology

T cells were isolated by negative selection (Neg Sel) or positive selection (Pos Sel) using EasySep™ or a competitor’s column-based technology. EasySep™ isolation yielded comparable or better purity (A) and recovery (B) to the competitor’s system. Data shown as mean ± SEM; n = 3.

Figure 2. EasySep™ Yields Equivalent or Better Purities and Recoveries of Mouse CD11b+ Cells Compared to a Column-Based Technology

CD11b+ cells were isolated from mouse spleen by positive selection using EasySep™ or a competitor’s column-based kit. The competitor’s protocol was followed using their standard protocol or their optional “high purity” protocol, which recommended an additional column. EasySep™ isolation yielded comparable or better purity (A) and recovery (B). Data shown as mean ± SEM; n = 4 - 6.

Figure 3. Cells Isolated Using EasySep™ Show Comparable Viability to Starting Samples

Immune cells were isolated from processed leukapheresis or peripheral blood samples using EasySep™ positive selection or negative selection kits. Pre- and postisolation samples were stained with the cell viability dye 7-AAD and appropriate cell surface markers, and were assessed by flow cytometry. Cells isolated using EasySep™ showed no significant decrease in viability compared to the starting samples. Data shown as mean ± SEM; n = 3 - 7.


Isolated Cells Respond Appropriately to Stimuli

Figure 4. T Cells Isolated Using EasySep™ Show an Appropriate Activation Phenotype

Human T cells were isolated from peripheral blood mononuclear cells (PBMCs) by negative selection (Neg Sel) or positive selection (Pos Sel) using EasySep™ or a competitor’s column-based system. Isolated T cells were assessed for CD25 expression immediately after isolation (Day 0) and after 3 days in culture with or without anti-CD3/CD28 stimulation. (A) At Day 0, isolated T cells expressed similar levels of CD25 compared to CD3+ cells in unmanipulated PBMCs. (B) At Day 3, cells remained unactivated in the absence of stimulation, while stimulated cells expressed the activation marker CD25. Data shown as mean ± SEM; n = 3.

Figure 5. Central Memory and Effector Memory CD4+ T Cells Isolated by EasySep™ Produce Cytokines When Stimulated

T cell subsets were isolated using the EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit. Isolated cells were cultured in medium with or without PMA and ionomycin stimulation for 4 hours. T cell subsets were assessed for the expression of IL-2 (A) and IFNγ (B) by intracellular flow cytometry. In the absence of stimulation, T cell subsets produced low levels of IL-2 and IFNγ. (A) When stimulated, central memory (CM) and effector memory (EM) CD4+ T cell subsets showed significantly higher IL-2 expression compared to naïve T cells. (B) EM CD4+ T cells expressed higher levels of IFNγ than CM CD4+ T cells when stimulated. Both CM and EM CD4+ T cell subsets showed higher IFNγ expression than naïve T cells. Data shown as mean ± SEM; n = 3.

Carefully Optimized Protocols

EasySep™ protocols and reagents are optimized to ensure robust and optimal performance across samples. Cocktails and magenetic particles are titrated to minimize non-specific binding, ensure proper cell labeling, and to avoid epitope blocking.

Figure 6. CD14 Epitopes Are Not Blocked Following Cell Isolation Using EasySep™

Cells were isolated by negative selection (Neg Sel) or positive selection (Pos Sel) methods using EasySep™ or a competitor’s column-based technology. Isolated cells were stained using an anti-CD14 antibody (clone M5E2), and assessed by flow cytometry. EasySep™-isolated cells showed similar levels of CD14 staining (MFI) compared to unprocessed CD14+ cells (Leukapheresis Start). Data shown as mean ± SEM; n = 4 - 5.



Isolated Cells Differentiate and Mature As Expected

EasySep™-isolated cells express appropriate surface markers following differentiation and maturation.

Figure 7. Monocytes Isolated Using EasySep™ Differentiate and Mature Appropriately Upon Stimulation

Human monocytes were isolated using EasySep™ or competitor products and then cultured and differentiated into mature dendritic cells (DCs). On Day 0, cells from a leukapheresis sample were plated and monocytes were adherence-selected for 2 hours. Non-adherent cells were washed away and adherent cells were cultured for 7 days. On Day 5, cells were cultured with maturation supplement for 2 days (mature DCs) or without maturation supplement (immature DCs). The expression of CD80, CD83, and CD86 in immature and mature DCs was determined by flow cytometry. At Day 7, cells expressed the mature DC markers CD80, CD83, and CD86.



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