EasySep™

A Smarter, More Efficient Way to Isolate Cells

Working efficiently is key to overcoming the demands of scientific research. That’s why our scientists developed a smarter, more efficient way to isolate cells. EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system—for fast and easy isolation of highly purified cells that are ready for downstream applications. This versatile immunomagnetic cell separation technology enables positive selection, negative selection, or cell depletion.

EasySep™ can be used to isolate cells in as little as 8 minutes from a variety of species (e.g. human, mouse, non-human primate, rat, rabbit, cow and pig) and sample sources (e.g. splenocytes, lymph nodes, PBMCs, whole blood, leukopaks, cord blood, and bone marrow).*

View All EasySep™ Cell Separation Kits >

How Does EasySep™ Cell Separation Work?

Cells are targeted for either removal (negative selection and depletion) or positive selection using antibody complexes directed to specific cell surface antigens. The antibody complexes link targeted cells to EasySep™ magnetic particles. Labeled cells are pulled to the sides of the tube when the sample is placed in an EasySep™ cell separation magnet, while untouched cells can be simply poured or pipetted off into a new tube.

Why Use EasySep™ to Isolate Cells?

  • Isolate cells in as little as 8 minutes with a simple pour.
  • Achieve up to 99% cell purities with high recoveries.
  • Obtain viable, functional cells without the need for columns and washes.
  • Isolate cells from virtually any sample source, including whole blood and Leukopaks.

Is EasySep™ a Well-Accepted Cell Isolation Method?

Since its launch in 2002, EasySep™ has been used in thousands of labs worldwide to quickly and easily isolate highly purified cells for a wide variety of applications. More immunologists are making the switch to EasySep™ and freeing up time to accomplish more in the lab. Switching your cell isolation technology to EasySep™ is easier than you think.



We use EasySep™ because of its ease of use and the high purity that we get from the preparation. I especially like the negative selection kits because it allows us to keep the cells that we collect as undisturbed as possible

Eric, MD/PhD Student

Already an EasySep™ user? Explore our next-generation EasySep™ kits and make sure your lab is using the most up-to-date kits.

EasySep™ Human Cell Separation Kits

EasySep™ Human Cell Isolation Kits

EasySep™ Human Cell Isolation Kits

Typical Sample Source:

Protocol Time:

As Little As 8 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • High purity (up to 98%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™

EasySep™ Human Positive Selection Kits

EasySep™ Human Positive Selection Kits

Typical Sample Source:

Protocol Time:

As Little As 15 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • High purity (up to 99%)
  • Column-free
  • Can be fully automated using RoboSep™

EasySep™ Direct Cell Isolation Kits

EasySep™ Direct

Typical Sample Source:

Protocol Time:

As Little As 20 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • 99.9% RBC depletion without density gradient centrifugation, lysis or sedimentation
  • High purity (up to 99%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis

EasySep™ Release Cell Separation Kits

EasySep™ Release

Typical Sample Source:

Protocol Time:

As Little As 29 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • High purity (up to 99%)
  • Column-free
  • Magnetic particle-free cells
  • Flexibility to perform sequential positive selections to isolate unique and rare cell types
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*Certain products are only available in select territories. Please contact your local Sales representative or the Product & Scientific Support team at techsupport@stemcell.com for further information.


They are very efficient kits and I can perform my isolations quickly without compromising on cell quality or viability.

Payel, Postdoctoral Fellow


EasySep™ Mouse and Rat Cell Separation Kits

EasySep™ Mouse Cell Isolation Kits

Typical Sample Source:

Spleen, Bone Marrow, Whole Blood, Lymph Nodes

Protocol Time:

As Little As 15 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • Fast and easy
  • High purity (up to 98%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™

EasySep™ Mouse Positive Selection Kits

Typical Sample Source:

Spleen, Bone Marrow, Whole Blood, Lymph Nodes

Protocol Time:

As Little As 15 Minutes

Selection Method:

Positive Selection

Features/Advantages:

  • Fast and easy
  • High purity (up to 99%)
  • Column-free
  • Can be fully automated using RoboSep™

EasySep™ Rat Cell Isolation Kits

Typical Sample Source:

Spleen, Lymph Nodes, Whole Blood

Protocol Time:

As Little As 13 Minutes

Selection Method:

Negative Selection

Features/Advantages:

  • Fast and easy
  • High purity (up to 99%)
  • Column-free
  • Untouched cells immediately ready for downstream analysis
  • Can be fully automated using RoboSep™
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I currently use the EasySep™ Mouse T Cell Isolation Kit, CD4+ T Cell Isolation Kit, and CD8+ T Cell Isolation Kit. I have used different isolation kits over the years and these yield superior results, in my opinion.

Cori, PhD Student


EasySep™ for Other Species and Cell Types

EasySep™ cell separation technology can also be used to isolate cells from a variety of other species, including non-human primate, rabbit, cow and pig. If you are working with specific rare cell types, non-standard species, or special applications, you can explore our custom cell isolation solutions or contact us at info@stemcell.com to discuss your specific cell separation needs.
View all products and try EasySep™ in your own lab.

Automated Cell Separation

RoboSep Cell Separation Instruments

EasySep™ cell separation technology can be automated using RoboSep™. Minimize sample handling and perform simultaneous and sequential cell isolations from multiple samples using RoboSep™-S and RoboSep™-16 instruments.

Learn More >

Related Resources

EasySep Magnetic Cell Separation and Cell Isolation

Magnetic Cell Separation

Find answers to three commonly asked questions about immunomagnetic cell separation.

Read Now >

Choosing Positive or Negative Cell Separation

Choosing a Positive or Negative Selection Approach

Read more about antibody labeling-based cell separation methods, including immunomagnetic and immunodensity cell separation.

Learn More >

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