Activation, Expansion, Maintenance and Differentiation of Immune Cells
Ensure optimal activation, expansion, and differentiation of immune cell subsets using ImmunoCult™ products. These immune cell culture media, activators, and supplements allow you to culture various cell types, including T cells, B cells, dendritic cells, and macrophages, under defined stimulatory conditions for consistent and reliable results.
Why Use ImmunoCult™?
Activate, expand, or differentiate immune cells in culture conditions optimized to promote high yield and frequency.
Minimize variation by using serum-free and animal-component free culture conditions.
Consistently achieve high yields of immune cells with the desired phenotype and function.
Mix and match media, activators, and supplements to suit your specific research needs.
Soluble antibody complexes that recognize and cross-link CD3 and co-stimulatory molecules on the surface of the T cell in the absence of magnetic beads or feeder cells. It can be used in combination with ImmunoCult™-XF T Cell Expansion Medium or any other media for culturing human T cells.
Robust activation and expansion of human T cells without the use of magnetic beads, feeder cells or antigen
Provides a gentle activation stimulus that maintains high viability of activated and expanded T cells
Serum-free and xeno-free medium optimized for the in vitro culture and expansion of human T cells isolated from peripheral blood. It can be used in combination with ImmunoCult™ T Cell Activators for bead-free activation of T cells.
No need to supplement the medium with serum
Supports robust T cell expansion at levels comparable to those in serum-containing media
Maintains similar proportions of CD4+/CD8+ cells to the start of culture after expansion
Expanded T cells are able to produce cytokines including IFN-gamma and IL-4
Differentiation supplements containing human cytokines and blocking antibodies formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into Th1, Th2 or Treg cells.
Formulated for the induction of human T helper cell differentiation
Optimized for use with ImmunoCult™-XF T Cell Expansion Medium
Support significantly higher levels of memory T cells in comparison to competitor differentiation kits
Supplied as a 50X concentrate; after thawing and mixing, the tube contents can be added directly to medium
Differentiation supplements containing cytokines and blocking antibodies formulated to promote the differentiation of mouse naïve CD4+ T cells into Th1, Th2 or Treg cells.
Optimized for the induction of Th1, Th2 or Treg cells from naïve CD4+ T cells isolated from the spleen of a C57BL/6 mouse
Supplied as a 100X concentrate; after thawing and mixing, the tube contents can be added directly to medium
Reagents for T Cell Therapy Research
The complex process of producing human T cells for cell therapy offers opportunities to optimize for maximum yield while retaining desired end phenotype and function. Explore additional optimized products for human T cell research, including tools for T cell isolation, genome editing, and cryopreservation.
Serum-free and animal component-free supplement formulated to promote robust in vitro expansion of human peripheral blood B cells in the absence of serum, feeder cells, or specialized culture plates. The supplement contains recombinant proteins and chemically defined components that support the activation and expansion of human B cells and their maturation to plasma cells.
Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
ImmunoCult™ Human B Cell Expansion Kit comprises ImmunoCult™-XF B Cell Base Medium and ImmunoCult™-ACF Human B Cell Expansion Supplement, which together ensure consistent activation and expansion of human B cells and their maturation to plasma cells. B cells can be harvested and used directly in downstream applications after just one week or less in culture.
Expand B cells in culture conditions optimized to promote high yield and frequency
Minimize variation by using serum-free and animal-component free culture conditions
Consistently achieve high yields of B cells
ImmunoCult™ for Dendritic Cell Generation
Streamline your research on dendritic cells (DCs) with products designed for robust generation of mature DCs from isolated monocytes.
Culture, differentiation and maturation of dendritic cells (DCs)
Serum-free and animal-component free (ACF) medium optimized for the in vitro culture and differentiation of human monocytes into dendritic cells. It can be used in combination with ImmunoCult™ DC Differentiation and Maturation Supplements.
No need to supplement the medium with serum
Supports DC differentiation and maturation
Can be used on its own or combined with differentiation and maturation supplements
Serum-free medium optimized for the in vitro culture and differentiation of human monocytes into macrophages. This medium can be combined with the appropriate cytokines and stimuli to differentiate M1 and M2a macrophages.
Serum-free medium without the need to supplement with serum
Supports robust macrophage differentiation
High yields of macrophages with the desired phenotype and function
Collect M1 or M2 macrophages in as little as 6 days
Optimization of Human T Cell Expansion Protocol: Effects of Early Cell Dilution
Tools to Streamline your Dendritic Cell Research
Genome Editing of Human Primary T Cells Using CRISPR-Cas9