ImmunoCult™ Mouse Th1 Differentiation Supplement

Supplement for the differentiation of mouse naïve CD4+ T cells into Th1 cells

ImmunoCult™ Mouse Th1 Differentiation Supplement

Supplement for the differentiation of mouse naïve CD4+ T cells into Th1 cells

From: 647 USD
Catalog #
10953_C
Supplement for the differentiation of mouse naïve CD4+ T cells into Th1 cells

Product Advantages


  • Optimized for the induction of Th1 cells from naïve CD4+ T cells isolated from the spleen of a C57BL/6 mouse

  • Supplied as a 100X concentrate; after thawing and mixing, the tube contents can be added directly to medium

Overview

ImmunoCult™ Mouse Th1 Differentiation Supplement contains a combination of recombinant mouse cytokines and a blocking monoclonal antibody formulated to promote the differentiation of mouse naïve CD4+ T cells into Th1 cells. This supplement is intended for use with RPMI 1640 Medium (Catalog #36750) containing fetal bovine serum and other additives, as well as anti-mouse CD3 and anti-mouse CD28 monoclonal antibodies as activating agents.

This product is recommended for:
• Research into the regulation of mouse naïve T cell differentiation and Th1 function
• Development of procedures to expand and polarize naïve T cells toward a Th1 subset
Contains
• Recombinant mouse interleukin 2 (IL-2)
• Recombinant mouse interleukin 12 (IL-12)
• Rat anti-mouse interleukin 4 (anti-IL-4)
Subtype
Supplements
Cell Type
T Cells, T Cells, CD4+
Species
Mouse
Application
Cell Culture, Differentiation, Expansion
Brand
ImmunoCult
Area of Interest
Immunology

Data Figures

Figure 1. ImmunoCult™ Mouse Th1 Differentiation Supplement Produces CD4+IFNγ+T-Bet+ Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarized cultures), or medium supplemented with ImmunoCult™ Mouse Th1 Differentiation Supplement (Polarized cultures ) for 5 days. Cells were subsequently stimulated with PMA/Ionomycin, in conjunction with brefeldin A treatment, followed by staining with anti-CD4, anti-IFNγ, anti-T-bet and a viability dye and analyzed by flow cytometry. Shown is the expression of IFNγ and T-bet, back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+IFNγ+T-Bet+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Th1 Differentiation Supplement (44 ± 6%) compared to non-polarized cells (2 ± 1%) (p < 0.001; n = 13). Data from experimental groups were compared using a paired T-test.

Figure 2. ImmunoCult™ Mouse Th1 Differentiation Supplement Expands Total Nucleated Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Th1 Differentiation Supplement for 5 days. A 4.5-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Th1 Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 11 - 20, *p < 0.05. Data from experimental groups were compared using a paired T-test).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
10953
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
10953
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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