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ImmunoCult™ Mouse Th1 Differentiation Supplement

Supplement for the differentiation of mouse naïve CD4+ T cells into Th1 cells

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ImmunoCult™ Mouse Th1 Differentiation Supplement

Supplement for the differentiation of mouse naïve CD4+ T cells into Th1 cells

1 mL
Catalog #10953
539 USD

Required Products

Overview

ImmunoCult™ Mouse Th1 Differentiation Supplement contains a combination of recombinant mouse cytokines and a blocking monoclonal antibody formulated to promote the differentiation of mouse naïve CD4+ T cells into Th1 cells. This supplement is intended for use with RPMI Medium (Catalog #36750) containing fetal bovine serum and other additives, as well as anti-mouse CD3 and anti-mouse CD28 monoclonal antibodies as activating agents.

This product is recommended for:
• Research into the regulation of mouse naïve T cell differentiation and Th1 function
• Development of procedures to expand and polarize naïve T cells toward a Th1 subset
Advantages:
• Optimized for the induction of Th1 cells from naïve CD4+ T cells isolated from the spleen of a C57BL/6 mouse
• Supplied as a 100X concentrate; after thawing and mixing, the tube contents can be added directly to medium
Contains:
• Recombinant mouse interleukin 2 (IL-2)
• Recombinant mouse interleukin 12 (IL-12)
• Rat anti-mouse interleukin 4 (anti-IL-4)
Subtype:
Supplements
Cell Type:
T Cells; T Cells, CD4+
Species:
Mouse
Application:
Cell Culture; Differentiation; Expansion
Brand:
ImmunoCult
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(4)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. ImmunoCult™ Mouse Th1 Differentiation Supplement Produces CD4+IFNγ+T-Bet+ Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarized cultures), or medium supplemented with ImmunoCult™ Mouse Th1 Differentiation Supplement (Polarized cultures ) for 5 days. Cells were subsequently stimulated with PMA/Ionomycin, in conjunction with brefeldin A treatment, followed by staining with anti-CD4, anti-IFNγ, anti-T-bet and a viability dye and analyzed by flow cytometry. Shown is the expression of IFNγ and T-bet, back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+IFNγ+T-Bet+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Th1 Differentiation Supplement (44 ± 6%) compared to non-polarized cells (2 ± 1%) (p < 0.001; n = 13). Data from experimental groups were compared using a paired T-test.

Figure 2. ImmunoCult™ Mouse Th1 Differentiation Supplement Expands Total Nucleated Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Th1 Differentiation Supplement for 5 days. A 4.5-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Th1 Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 11 - 20, *p < 0.05. Data from experimental groups were compared using a paired T-test).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.