ImmunoCult™ Mouse Th2 Differentiation Supplement

Supplement for the differentiation of mouse naïve CD4+ T cells into Th2 cells
ImmunoCult™ Mouse Th2 Differentiation Supplement

Supplement for the differentiation of mouse naïve CD4+ T cells into Th2 cells

1 mL
Catalog # 10955
594 USD
Required Products
  1. RPMI 1640 Medium
    RPMI 1640 Medium

    RPMI 1640 medium

Overview

ImmunoCult™ Mouse Th2 Differentiation Supplement contains a combination of recombinant mouse cytokines and a blocking monoclonal antibody optimized to promote the differentiation of mouse naïve CD4+ T cells into Th2 cells. This supplement is intended for use with RPMI 1640 Medium (Catalog #36750) containing fetal bovine serum and other additives, as well as anti-mouse CD3 and anti-mouse CD28 monoclonal antibodies as activating agents.

This product is recommended for:
• Research into the regulation of mouse naïve T cell differentiation and Th2 function
• Development of procedures to expand and polarize naïve T cells toward a Th2 subset
Advantages
• Optimized for the induction of Th2 cells from naïve CD4+ T cells isolated from the spleen of a BALB/c mouse
• Supplied as a 100X concentrate; after thawing and mixing, the tube contents can be added directly to medium.
Contains
• Recombinant mouse interleukin 2 (IL-2)
• Recombinant mouse interleukin 4 (IL-4)
• Rat anti-mouse interferon-gamma (anti-IFN-γ)
Subtype
Supplements
Cell Type
T Cells, T Cells, CD4+
Species
Mouse
Application
Cell Culture, Differentiation, Expansion
Brand
ImmunoCult
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse Th2 Differentiation Supplement
Catalog #
10955
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Mouse Th2 Differentiation Supplement
Catalog #
10955
Lot #
All
Language
English

Educational Materials (4)

Brochure
Mouse Cell Isolation
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. ImmunoCult™ Mouse Th2 Differentiation Supplement Produces CD4+GATA3+IL-4+ Cells Under Th2 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarized cultures), or in medium supplemented with ImmunoCult™ Mouse Th2 Differentiation Supplement (Polarizing cultures) for 6 days. Cells were subsequently stimulated with PMA/Ionomycin, in conjunction with monensin treatment, followed by staining with anti-CD4, anti-IL-4, anti-GATA3 and a viability dye and analyzed by flow cytometry. Shown is the expression of GATA3 and IL-4 back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+IL-4+GATA3+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Th2 Differentiation Supplement (25 ± 3%) compared to non-polarized cells (4 ± 1%) (p < 0.001; n = 10). Data from experimental groups were compared using a paired T-test.

Figure 2. ImmunoCult™ Mouse Th2 Differentiation Supplement Expands Total Nucleated Cells Under Th2 Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Th2 Differentiation Supplement for 6 days. A 62-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Th2 Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 11, ***p < 0.001. Data from experimental groups were compared using a paired T-test).

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