ImmunoCult™ Human Th2 Differentiation Supplement

Serum-free culture supplement for the differentiation of human naïve CD4+ T cells into Th2 cells
ImmunoCult™ Human Th2 Differentiation Supplement

Culture supplement for the differentiation of human naïve CD4+ T cells into Th2 cells

1 mL
Catalog # 10975
301 USD

Overview

ImmunoCult™ Human Th2 Differentiation Supplement contains a human cytokine and blocking antibody formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into Th2 cells. ImmunoCult™ Human Th2 Differentiation Supplement is intended for use with ImmunoCult™-XF T Cell Expansion Medium and ImmunoCult™ Human CD3/CD28 T Cell Activator.

This product is recommended for:
• Research into the regulation of human naïve T cell differentiation and Th2 function
• Development of procedures to expand and polarize naïve T cells toward a Th2 subset
Advantages
• Formulated for the induction of human Th2 differentiation
• Optimized for use with ImmunoCult™-XF T Cell Expansion Medium
• Supports significantly higher levels of memory T cells in comparison to competitor Th2 differentiation kit
• Supports up to 3X higher expansion of IL-4-producing CD4+ T cells in comparison to competitor Th2 differentiation kit
• Supplied as a 50X concentrate; after thawing and mixing, the tube contents can be added directly to medium
Contains
• Recombinant human interleukin 4 (IL-4)
• Mouse anti-human interferon-gamma (anti-IFN-γ)
Subtype
Supplements
Cell Type
T Cells, T Cells, CD4+
Species
Human
Application
Cell Culture, Differentiation, Expansion
Brand
ImmunoCult
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Human Th2 Differentiation Supplement
Catalog #
10975
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Human Th2 Differentiation Supplement
Catalog #
10975
Lot #
All
Language
English

Educational Materials(6)

Brochure
Isolate Human Immune Cells
Brochure
Tools For Your Immunology Research
Brochure
Human T Cell Research Product Workflow
Wallchart
Antigen Processing and Presentation
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Cell Types in Human Peripheral Blood

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. ImmunoCult™ Human Th2 Differentiation Supplement Produces Differentiated CD4+CD45RA-CD45RO+ Cells Under Th2 Polarizing Conditions

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555). Cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th2 Differentiation Supplement (Catalog #10975), cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 14 days. Shown are flow cytometry results of cell samples stained and analyzed for expression of CD45RA and CD45RO (A) before and (B) after 14 days of culture. After 14 days the majority of cells in culture display a memory T cell phenotype (CD45RA-CD45RO+).

Figure 2. ImmunoCult™ Human Th2 Differentiation Supplement Produces More Differentiated CD4+CD45RA-CD45RO+ Cells Under Th2 Polarizing Conditions Than Competitor

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) and cultured with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th2 Differentiation Supplement (Catalog #10975) in ImmunoCult™ and differentiated (CD45RA-CD45RO+) cells after culture was compared after staining cells for CD45RA and CD45RO and analysis by flow cytometry. The percentage of cells expressing a naïve phenotype was similar and the percentage of cells expressing a differentiated phenotype was significantly higher with ImmunoCult™ than with competitor medium and activator. Shown are the differences in the percentage of naïve and mature T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; *p < 0.05; paired t-test on logit transformed data; n = 4).

Figure 3. Culture with ImmunoCult™ Human Th2 Differentiation Supplement Produces Similar Percentages of Cells Expressing IFNγ and IL-4 as Culture Under Competitor Conditions

Naïve CD4+ T cells were cultured for 13 days in ImmunoCult™ activator, supplement and medium, or competitor medium and activator using the protocol described in Figure 2. After culture, differentiated CD4+ T cells (CD45RA-CD45RO+) were analyzed for (A) IFNγ and (B) IL-4 expression, following stimulation with PMA and Ionomycin for 4 hours. Similar percentages of cells generated with ImmunoCult™ expressed intracellular IFNγ and IL-4 as compared to those generated under competitor conditions. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; paired t-test on logit transformed data; n = 4).

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