ImmunoCult™-ACF Human B Cell Expansion Supplement
Overview
ImmunoCult™-ACF Human B Cell Expansion Supplement is a 50X supplement that can be added to a base medium such as ImmunoCult™-XF B Cell Base Medium (Catalog #100-0646) to prepare a complete serum-free medium for culturing and expanding human B cells.
⦁ Serum-free and animal component-free supplement
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
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Document Type
Product Information Sheet
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Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
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Catalog # 10974 |
Lot # All |
Language English |
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Document Type
Safety Data Sheet
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Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
|
Catalog # 10974 |
Lot # All |
Language English |
Educational Materials(6)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™-ACF Human B Cell Expansion Supplement
B cells isolated from human peripheral blood mononuclear cells (PBMCs) (Leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF B Cell Base Medium was used as a base medium. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1,190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.
Figure 2. Light Microscopy Image of Cultured Human B Cells
B cells isolated from human PBMCs (Leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF B Cell Base Medium was used as a base medium. The cells were passaged on day 4 after seeding and imaged at 40x magnification on day 6.
Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™-ACF Human B Cell Expansion Supplement
Memory B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF B Cell Base Medium was used as a base medium. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each time point. Data represent the mean ± 1 SD of triplicate cultures for the same donor.
Figure 4. Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™-ACF Human B Cell Expansion Supplement
Naive B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF B Cell Base Medium was used as a base medium. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each time point. Data represent the mean ± 1 SD of triplicate cultures for the same donor.
Figure 5. Expansion of Human Pan-B Cells Cultured with Various Base Media Supplemented with ImmunoCult™-ACF Human B Cell Expansion Supplement or 10% FBS
Pan-B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit and seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with various base media supplemented with either ImmunoCult™-ACF Human B Cell Expansion Supplement or 10% FBS. The cells were passaged every 3 - 4 days and the fold expansion of viable cells was calculated at each time point. Data represent the mean + 1SD for n = 3 donors (each culture condition was performed in triplicate).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
