On-Demand Human Immune Cell Isolation Course

Register now for an on-demand course on human immune cell isolation.

In this self-paced, virtual course, you will learn how to prepare human blood samples, perform cell separation with EasySep™, and analyze the performance of your isolations.

Instructor Bio

Dr. Lu Lu, Specialist, Immunology, Scientific Support

Lu is an Immunology Specialist in the Scientific Support Department at STEMCELL Technologies. Lu obtained her PhD in Virology from the University of Hong Kong with a focus on using adjuvants to improve vaccine effectiveness. Prior to joining STEMCELL, she was an Assistant Research Officer and led COVID-19-related team projects. At STEMCELL, Lu provides frontline support with our product portfolios to the global customer base and sales staff, supporting research, biotech, and diagnostic labs. She specializes in STEMCELL’s immunology product portfolio, including cell separation and immune cell culture products, and is also a trainer in corporate training programs with a focus on immunology products and protocols.

Course Curriculum

Module 1: Preparing Cells for EasySep™ - Common Human Peripheral Blood Samples and How to Use Them with EasySep™

Common human peripheral blood samples compatible with EasySep™
How to use human blood samples with EasySep™
How to process leukopaks (LP) for EasySep™
How to process Leukocyte Reduction System Cones (LRSC) for EasySep™

Mini Quiz: Test Your Knowledge

Estimated time for completion: 20 minutes

Module 2: Preparing Cells for EasySep™ - Frequently Used Processing Methods for Human Blood Samples

Common anticoagulants used for blood sample collection
How to isolate PBMCs, cryopreserve them, and thaw them for isolation
How to prepare a buffy coat
How to remove red blood cells (RBCs)
How to remove platelets

Mini Quiz: Test Your Knowledge

Estimated time for completion: 25 minutes

Module 3: EasySep™ Technology Overview

What EasySep™ cell separation technology is
What EasySep™ strategies are available
What cells can be isolated with EasySep™
How to choose an EasySep™ product for your isolation

Mini Quiz: Test Your Knowledge

Estimated time for completion: 20 minutes

Module 4: Key Steps When Using EasySep™

How to execute the key steps when performing cell separation with EasySep™, including:
- Before cell separation with EasySep™: Storing the kit components, preparing the starting sample, and selecting a magnet
- During cell separation with EasySep™: Adding reagents and buffer, removing the supernatant after magnetic separation, and resuspending the particle-bound cells

Mini Quiz: Test Your Knowledge

Estimated time for completion: 20 minutes

Module 5: Performance Analysis after EasySep™ Cell Isolation

How to assess the separation performance after EasySep™ by purity and recovery analysis

Mini Quiz: Test Your Knowledge

Estimated time for completion: 20 minutes

Final Assessment

Complete this final assessment to test your knowledge and receive a certificate of completion.

Estimated time for completion: 20 minutes

This on-demand course is divided into five modules and a final assessment. Following completion of the course, trainees can choose to receive a certificate of completion. Note that this is not an accredited course and cannot be used to receive Continuing Education (CE) credits.