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Free Your Isolated Cells from Particles

Use EasySep™ Release to Positively Select Cells

You can now positively select immune cells and then release bound magnetic particles from your isolated cells. EasySep™ Release combines the speed and ease of the EasySep™ cell isolation platform with the flexibility of the new Releasable RapidSpheres™ for the separation of particle-free, highly purified immune cells. Mix and match with non-releasable EasySep™ cell isolation kits that use Dextran RapidSpheres™ to isolate unique and rare cell types by performing sequential positive selections.

Why Use EasySep™ Release?

  • Culture and analyze your positively selected cells without magnetic particles.
  • Perform sequential positive selections to isolate unique and rare cell types.
  • Obtain highly purified cells free of magnetic particles in under 30 minutes.
  • With no columns, the simple EasySep™ pour-off is gentle on your cells.
  • Achieve purities of up to 99%.

Typical EasySep™ Release Protocol


Table 1. Typical purities of immune cells positively selected using EasySep™ Release Positive Selection Kits

Positive Selection
Typical Purity (mean ± SD)
Catalog #
Human CD3
98.7 ± 0.9%
Human CD4
96.1 ± 4.1%
Human CD19
97.7 ± 2.3%
Human PE*
N/A
Mouse PE*
N/A
Human Biotin*
N/A
Mouse Biotin*
N/A

*These kits are designed to isolate cells that are labeled with either PE- or biotin-conjugated antibodies by immunomagnetic positive selection. They may also be used for cell isolation from other species (e.g. rat) provided a suitable blocker is available.


Data

Typical EasySep™ Release Positive Selection Purities

Typical EasySep™ Release Human CD3 Positive Selection

Figure 1. Typical EasySep™ Release Human CD3 Positive Selection.

Starting with a single-cell suspension of human PBMCs, the CD3+ cell content of the isolated fraction is typically 98.7 ± 0.9% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 38.4% and 99.0%, respectively.

Typical EasySep™ Release Human CD19 Positive Selection

Figure 2. Typical EasySep™ Release Human CD19 Positive Selection.

Starting with a single-cell suspension of human PBMCs, the CD19+ cell content of the isolated fraction is typically 97.7 ± 2.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 6.2% and 98.0%, respectively.

Isolation of Mouse Lung Epithelial Cells

Figure 3. Isolation of Mouse Lung Epithelial Cells.

Mouse lung cells were firstly labeled with PE-conjugated anti-mouse CD326 (EpCAM) and biotin-conjugated CD45 antibodies. EpCAM+ cells were positively selected using EasySep™ Release Mouse PE Positive Selection Kit, and subsequently, CD45+ contaminating leukocytes were depleted using EasySep™ Mouse Biotin Positive Selection Kit. Isolated EpCAM+CD45- cells were 86% pure.

Isolation of Mouse CD4+CD304+ Cells.

Figure 4. Isolation of Mouse CD4+CD304+ Cells.

Mouse spleen cells were labeled with PE-conjugated CD304 (Neuropilin-1). CD304+ cells were positively selected using EasySep™ Release Mouse PE Positive Selection Kit, and subsequently, CD4+ cells were negatively selected using EasySep™ Mouse CD4+ T Cell Isolation Kit. Isolated CD4+CD304+ cells were 92% pure.