Free Your Isolated Cells from Particles
Use EasySep™ Release to Positively Select Cells
Why Use EasySep™ Release?
- Culture and analyze your positively selected cells without magnetic particles.
- Perform sequential positive selections to isolate unique and rare cell types.
- Obtain highly purified cells free of magnetic particles in under 30 minutes.
- With no columns, the simple EasySep™ pour-off is gentle on your cells.
- Achieve purities of up to 99%.
Typical EasySep™ Release Protocol
Table 1. Typical purities of immune cells positively selected using EasySep™ Release Positive Selection Kits
*This kit can be used with human samples and samples from humanized mice.
**These kits are designed to isolate cells that are labeled with either APC-, PE- or biotin-conjugated antibodies by immunomagnetic positive selection. They may also be used for cell isolation from other species (e.g. rat) provided a suitable blocker is available.
Data
Typical EasySep™ Release Positive Selection Purities
Figure 1. Typical EasySep™ Release Human CD3 Positive Selection.
Starting with a single-cell suspension of human PBMCs, the CD3+ cell content of the isolated fraction is typically 98.7 ± 0.9% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 38.4% and 99.0%, respectively.
Figure 2. Typical EasySep™ Release Human CD19 Positive Selection.
Starting with a single-cell suspension of human PBMCs, the CD19+ cell content of the isolated fraction is typically 97.7 ± 2.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 6.2% and 98.0%, respectively.
Figure 3. Isolation of Mouse Lung Epithelial Cells.
Mouse lung cells were firstly labeled with PE-conjugated anti-mouse CD326 (EpCAM) and biotin-conjugated CD45 antibodies. EpCAM+ cells were positively selected using EasySep™ Release Mouse PE Positive Selection Kit, and subsequently, CD45+ contaminating leukocytes were depleted using EasySep™ Mouse Biotin Positive Selection Kit. Isolated EpCAM+CD45- cells were 86% pure.
Figure 4. Isolation of Mouse CD4+CD304+ Cells.
Mouse spleen cells were labeled with PE-conjugated CD304 (Neuropilin-1). CD304+ cells were positively selected using EasySep™ Release Mouse PE Positive Selection Kit, and subsequently, CD4+ cells were negatively selected using EasySep™ Mouse CD4+ T Cell Isolation Kit. Isolated CD4+CD304+ cells were 92% pure.
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