Free Your Isolated Cells from Particles

Use EasySep™ Release to Positively Select Cells

You can now positively select immune cells and then release bound magnetic particles from your isolated cells. EasySep™ Release combines the speed and ease of the EasySep™ cell isolation platform with the flexibility of the new Releasable RapidSpheres™ for the separation of particle-free, highly purified immune cells. Mix and match with non-releasable EasySep™ cell isolation kits that use Dextran RapidSpheres™ to isolate unique and rare cell types by performing sequential positive selections.

Why Use EasySep™ Release?

  • Culture and analyze your positively selected cells without magnetic particles.
  • Perform sequential positive selections to isolate unique and rare cell types.
  • Obtain highly purified cells free of magnetic particles in under 30 minutes.
  • With no columns, the simple EasySep™ pour-off is gentle on your cells.
  • Achieve purities of up to 99%.

Typical EasySep™ Release Protocol


Table 1. Typical purities of immune cells positively selected using EasySep™ Release Positive Selection Kits

Positive Selection
Typical Purity (mean ± SD)
Catalog #
Human CD3
98.7 ± 0.9%
Human CD4
96.1 ± 4.1%
Human CD19
97.7 ± 2.3%
Human CD45*
Varies by sample and tissue source
Human APC**
N/A
Mouse APC**
N/A
Human PE**
N/A
Mouse PE**
N/A
Human Biotin**
N/A
Mouse Biotin**
N/A

*This kit can be used with human samples and samples from humanized mice.
**These kits are designed to isolate cells that are labeled with either APC-, PE- or biotin-conjugated antibodies by immunomagnetic positive selection. They may also be used for cell isolation from other species (e.g. rat) provided a suitable blocker is available.


Data

Typical EasySep™ Release Positive Selection Purities

Typical EasySep™ Release Human CD3 Positive Selection

Figure 1. Typical EasySep™ Release Human CD3 Positive Selection.

Starting with a single-cell suspension of human PBMCs, the CD3+ cell content of the isolated fraction is typically 98.7 ± 0.9% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 38.4% and 99.0%, respectively.

Typical EasySep™ Release Human CD19 Positive Selection

Figure 2. Typical EasySep™ Release Human CD19 Positive Selection.

Starting with a single-cell suspension of human PBMCs, the CD19+ cell content of the isolated fraction is typically 97.7 ± 2.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 6.2% and 98.0%, respectively.

Isolation of Mouse Lung Epithelial Cells

Figure 3. Isolation of Mouse Lung Epithelial Cells.

Mouse lung cells were firstly labeled with PE-conjugated anti-mouse CD326 (EpCAM) and biotin-conjugated CD45 antibodies. EpCAM+ cells were positively selected using EasySep™ Release Mouse PE Positive Selection Kit, and subsequently, CD45+ contaminating leukocytes were depleted using EasySep™ Mouse Biotin Positive Selection Kit. Isolated EpCAM+CD45- cells were 86% pure.

Isolation of Mouse CD4+CD304+ Cells.

Figure 4. Isolation of Mouse CD4+CD304+ Cells.

Mouse spleen cells were labeled with PE-conjugated CD304 (Neuropilin-1). CD304+ cells were positively selected using EasySep™ Release Mouse PE Positive Selection Kit, and subsequently, CD4+ cells were negatively selected using EasySep™ Mouse CD4+ T Cell Isolation Kit. Isolated CD4+CD304+ cells were 92% pure.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.