hPSC-Derived Mesoderm Cell Research

Many protocols have been developed to differentiate human embryonic (ES) and induced pluripotent stem (iPS) cells into specialized cell types for many applications, including disease modeling, drug discovery, toxicity screening and cell therapy. See MoreFor cells that are derived from the mesoderm, such as cardiomyocytes, osteoclasts, endothelial cells and chondrogenic cells, the generation of quality early mesoderm cells is essential. With our xeno-free differentiation medium , you can efficiently differentiate ES and iPS cell lines to early mesoderm in only 2 - 4 days.

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Brochure STEMdiff™ Mesoderm Induction Medium
Brochure STEMdiff™ Mesenchymal Progenitor Kit
Wallchart Directed Differentiation of Pluripotent Stem Cells
Tech Tip Enzyme-Free Passaging and Generation of Single Cells from Human ES and iPS Cells
Tech Tip Coating plates with Matrigel™ for pluripotent stem cell culture

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Products

Cell Culture Media and Supplements 4 (5)

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STEMdiff™ Kidney Organoid Kit

Serum-free medium kit for the culture of kidney organoids from hPSCs
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TeSR™-E6

Defined, serum-free, xeno-free medium for pluripotent stem cells
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TeSR™-E5

Defined, serum-free, xeno-free medium for pluripotent stem cells
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STEMdiff™ Mesoderm Induction Medium

Defined, xeno-free induction medium for early mesodermal differentiation

Small Molecules 4 (3)

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TTNPB

Retinoid pathway activator; Activates retinoic acid receptor (RAR)
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AM580

Retinoid pathway activator; Activates retinoic acid receptor (RAR) alpha
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CHIR99021

WNT pathway activator; Inhibits GSK3

Cytokines and Proteins 4 (10)

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Human Recombinant BMP-4

Bone morphogenetic protein 4
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Human Recombinant FGF-10 (KGF-2), ACF

Fibroblast growth factor 10, animal component-free
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Human Recombinant VEGF-165, ACF

Vascular endothelial growth factor, animal component-free
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Human Recombinant SCF, ACF

Stem cell factor, animal component-free

Antibodies 4 (4)

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Anti-Mouse OCT4 (OCT3) Antibody, Clone 40

Mouse monoclonal IgG1 antibody against human, mouse OCT4 (OCT3)
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Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20

Mouse monoclonal IgG2b antibody against human OCT4 (OCT3)
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Anti-Human SSEA-4 Antibody, Clone MC-813-70

Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4
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Anti-Human CD56 (NCAM) Antibody, Clone HCD56

Mouse monoclonal IgG1 antibody against human CD56 (NCAM)

Matrices and Substrates 4 (2)

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CellAdhere™ Dilution Buffer

Dilution buffer for matrix proteins, e.g. Vitronectin XF™
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Vitronectin XF™

Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions

Cultureware and General Supplies 4 (1)

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Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)

Tissue and Cell Culture Dissociation Reagents 4 (1)

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Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent

Cell Engineering and Molecular Tools 4 (2)

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qPCR Master Mix Kit

For probe-based assays and arrays
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Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array

For characterization of hPSC trilineage differentiation to endoderm, mesoderm, and ectoderm germ layers

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