MesenCult™ Mesenchymal Stromal Cell (MSC) Culture

Your High-Performance System for MSC Isolation, Culture & Differentiation

The MesenCult™ product line was developed as a comprehensive and integrated suite of products to help mesenchymal stem cell (MSC) researchers standardize their MSC culture system and overcome issues associated with cell culture media variability. Choose from a comprehensive range of specialty products for human and mouse MSCs designed to service scientists along the basic to translational research continuum.

MesenCult™ Workflow for MSC Derivation, Culture & Differentiation

Derive, culture, expand, differentiate, and cryopreserve MSCs using MesenCult™. The MesenCult™ product line offers a range of animal component-free and human platelet lysate- and serum-containing media for MSC research.

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Why Use MesenCult™?

  • Species-optimized media formulations provide superior performance compared to traditional and competitor media.
  • A comprehensive and integrated range of products is available for the enrichment, expansion, quantification (CFU-F Assay) and differentiation of human and mouse mesenchymal stem cells.
  • Standardized MSC culture media and conditions minimize variability and lead to increased reproducibility between experiments.
  • Isolate, culture, cryopreserve and differentiate human MSCs with animal component-free, serum-free formulations.

MesenCult™ Human MSC Media

Reduce variation by culturing and differentiating human MSCs in standardized serum-free and animal component-free MesenCult™ media formulations.

Animal Component-Free Media

Mesenchymal stem cells (MSCs) have potential utility in a range of cellular therapies, with applications relating to tissue engineering and regenerative medicine, and as vehicles for gene therapy. The limited global supply of fetal bovine serum (FBS) and concerns about immune rejection of transplanted cells or disease transmission make animal component-free media an attractive alternative when expanding cells to numbers for therapeutic purposes.

MesenCult™-ACF media together allow for the optimized isolation, clonogenic growth, cryopreservation, long-term expansion and differentiation of human MSCs derived from primary tissues (bone marrow and adipose). To date, this is the only complete and integrated culture system completely free of serum and other materials derived from human or animal sources. With animal component-free media, experimental variability is minimized, and the safety concerns associated with exposure to animal-derived materials in translational research are addressed.

Plus Medium

Product Type:

Serum-free and animal component-free


  • MSC isolation and expansion from primary human bone marrow or adipose tissue as well as culture-expanded cells
  • Long-term culture of MSCs (>8 passages)
  • Detection and enumeration of CFU-F from primary human bone marrow or adipose tissue


  • Animal component-free
  • Optimized for isolation of MSC directly from primary human tissue
  • Superior cell expansion compared to serum-containing medium
  • Significant reduction in hematopoietic cell contamination at early passages
  • Supports efficient clonogenic growth and long-term expansion of human MSCs
  • No adaptation required when transitioning MSCs from serum-containing medium

Freezing Medium

Product Type:

Serum-free and animal component-free


Cryopreservation of human MSCs


  • Serum-free and animal component-free
  • Reproducibly high recovery rates
  • Preserves human MSC multipotency and expansion capacities
  • Convenient, ready-to-use format
  • Optimized for MSCs previously expanded in MesenCult™-ACF Plus Medium, MesenCult™-hPL Medium, or MesenCult™ Proliferation Kit (Human)

Chondrogenic Differentiation Medium

Product Type:

Serum-free and animal component-free


Animal component-free, in vitro differentiation of MSCs into chondrocytes


  • Animal component-free (ACF) formulation
  • Robust chondrogenic differentiation with as few as 3 x105 MSCs per 3D pellet and as early as day 14
  • Strong expression of chondrogenic transcripts - Sox9, Acan, Col2a and Col10a; low expression of hypertrophic transcript Mmp13
  • Completes optimized ACF workflow for MSC isolation, expansion, cryopreservation and chondrogenic differentiation when used with other MesenCult™-ACF media
  • Compatible with MSCs previously expanded in MesenCult™-ACF Plus Medium, MesenCult™-hPL Medium, or MesenCult™ Proliferation Kit (Human)
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Explore More MesenCult™ Products for Human MSCs

Find more products for your human MSC research, including MesenCult™ Osteogenic Differentiation Kit for the differentiation of MSCs into osteoblasts, MesenCult™ Adipogenic Differentiation Kit for the differentiation of MSCs into adipocytes, and MesenCult™ Proliferation Kit for the detection of CFU-F.

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MesenCult™ Mouse MSC Media

Optimize the culture and differentiation of mouse MSCs with standardized MesenCult™ media and supplements.

MesenCult™ Expansion Kit (Mouse)

Product Type:

Medium and reagents to expand and enrich for mouse mesenchymal stem and progenitor cultures as early as passage 0


  • Derivation and expansion of mouse MSCs and MEFs
  • Enumeration of mouse MSCs and MEFs using the CFU-F assay


  • Fast expansion of mouse MSCs with robust enrichment as early as passage 0
  • Optimized for use with mouse bone marrow-, compact bone- and adipose-derived MSCs and MEFs
  • Obtain homogeneous mouse MSC cultures while maintaining tri-lineage differentiation potential
  • Rigorous raw material screening and quality control minimize lot-to-lot variability

Osteogenic Stimulatory Kit (Mouse)

Product Type:

Complete medium for differentiating mouse mesenchymal stem and progenitor cells and embryonic fibroblasts into osteoblasts


  • In vitro differentiation of mouse MSCs and mouse embryonic fibroblasts (MEFs) into osteoblasts
  • Bone development studies


  • Achieve unparalleled levels of osteogenic differentiation compared to competitor formulations
  • Superior expression of key osteogenic transcripts involved in bone differentiation and maturation
  • Robust bone matrix deposition (Alizarin Red staining)

MesenCult™ Adipogenic Differentiation Kit (Mouse)

MesenCult™ Adipogenic Differentiation Kit (Mouse)

Product Type:

Medium for the differentiation of mouse MSCs, ADSCs, and MEFs into adipocytes


  • In vitro differentiation of mouse mesenchymal stem and progenitor cells (MSCs), adipose tissue-derived MSCs (ADSCs), and mouse embryonic fibroblasts (MEFs) into cells of the adipogenic lineage


  • Compatible with mouse MSCs previously culture-expanded using the MesenCult™ Expansion Kit (Mouse).
  • Available in an easy-to-use two-component format.
  • Rigorous raw material screening and quality control minimize lot-to-lot variability.
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Scientific Resources

Mesenchymal stem cells

How to Create a Hypoxic Environment for Cell Culture

Learn how to mimic physiological conditions to support MSC growth in vitro with a Hypoxia Incubator Chamber.

Read Now >

Mesenchymal stem cell survey results

MSC Survey Results

Read what 450 researchers from around the world had to say in our survey on the state of the MSC field.

See Results Now >

Key Applications

Serum-Free Culture of Human Bone-Marrow Derived Mesenchymal Stem Cells

Miwa H et al. (2012) Xeno-free proliferation of human bone marrow mesenchymal stem cells. Cytotechnology 64(3): 301-8

Serum-Free Culture of Human Adipose-Derived Mesenchymal Stem Cells

Other Uses

Serum-Free Culture of Human Placenta-Derived Mesenchymal Stem Cells

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