MesenCult™ Osteogenic Stimulatory Kit (Mouse)
Complete medium for differentiating mouse mesenchymal stem cells and embryonic fibroblasts into osteoblasts
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Overview
Data Figures

Figure 1. Differentiation of mouse compact bone-derived mesenchymal stem cells (CB-MSCs) with the MesenCult™ Osteogenic Stimulatory Kit (Mouse)
Compact bone-derived MSCs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*

Figure 2. Compact bone-derived MSCs display robust bone matrix deposition following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse) as demonstrated by Alizarin Red-staining
Ca2+ deposits are detected with Alizarin Red.
For both the qPCR analysis and Alizarin-Red staining assay, the mouse compact bone-derived MSCs were cultured in vitro for three passages, purified with EasySep™ Mesenchymal Stem/Progenitor Cell Enrichment Kit (Mouse, Catalog #19771), and then cultured for another passage, prior to differentiation with the MesenCult™ Osteogenic Stimulatory Kit (Mouse).

Figure 3.Differentiation of mouse embryonic fibroblasts (MEFs) with MesenCult™ Osteogenic Stiumulatory Kit (Mouse)
MEFs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*

Figure 4. MEFs treated with MesenCult™ Osteogenic Stimulatory Kit (Mouse) displayed substantially increased alkaline phosphatase activity and bone matrix deposition*
Alkaline phosphatase activity is shown by Naphthol AS-MX Phosphate/Fast Red Violet LB Salt staining (red areas) while bone matrix deposition is shown by von Kossa staining (black areas).
*Versus a homemade osteogenic medium (mouse) from Lab A.
For the qPCR analysis and staining assays, MEFs were cultured in vitro for 20 days using MesenCult™ Osteogenic Stimulatory Kit (Mouse), homemade, or MesenCult™ Proliferation Kit (Mouse, Catalog #05511) as the negative control.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.