Mesenchymal Stem and Progenitor Cell Research

Mesenchymal stem cells (MSCs) are fibroblast-like cells isolated from bone marrow, adipose, umbilical cord blood and other tissues. See MoreDue to the low frequency at which MSCs occur in tissues, it may be desirable to enrich for MSCs from a mixed cell population. No single, unique marker allowing for MSC isolation has been reported, but rather a range of composite cell surface phenotypes are utilized. Consequently, typical isolation methods employ a strategy of enrichment via depletion of undesired cell types.

Obtain untouched mesenchymal stem cells from human bone marrow or mouse compact bone or select CD271+ MSCs from human bone marrow using cell isolation kits. Alternatively, choose primary cell products for MSC research, such as fresh whole cord blood.

Learn More

Video (5:34) Isolation of MSCs from Mouse Compact Bone
Video (5:51) How to Perform Cell Counts with a Hemacytometer
Tech Tip Total Nucleated Cell and Viable Cell Counts
Brochure Mesenchymal Stem Cells: MesenCult™: Your High-Performance System for MSC Isolation, Culture & Differentiation

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Products

Cell Isolation Products 4 (3)

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EasySep™ Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit

Immunomagnetic negative selection cell isolation kit
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EasySep™ Human CD271 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
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RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail

Immunodensity negative selection cocktail

Laboratory Equipment 4 (3)

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EasySep™ Magnet

Magnet for column-free immunomagnetic separation
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EasySep™ EasyStand™

Linkable stand for EasySep™ Magnet
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EasySep™ Multistand

Multi-magnet stand for column-free immunomagnetic separation

Tissue and Cell Culture Dissociation Reagents 4 (12)

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DisCoZyme 2, ACF

Animal component-free collagenase/dispase blend with high collagenase and high caseinase activity
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DisCoZyme 1, ACF

Animal component-free collagenase/dispase blend with high collagenase and high caseinase activity
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Collagenase Type I (0.25%)

Cell dissociation reagent
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Dispase, ACF

Animal component-free non-specific protease

Cell Culture Media and Supplements 4 (2)

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STEMdiff™ Mesenchymal Progenitor Kit

Defined culture kit for derivation and expansion of mesenchymal progenitor cells
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MesenCult™-ACF Plus Umbilical Cord Culture Kit

Animal component-free medium for derivation and expansion of human mesenchymal stromal cells from umbilical cord tissue

Cell Dyes and Detection Assay Kits 4 (10)

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ALDEFLUOR™ DEAB Reagent

ALDH enzyme inhibitor used as a control in the ALDEFLUOR™ assay system
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ALDEFLUOR™ Kit

For the identification, evaluation, and isolation of stem and progenitor cells expressing high levels of ALDH
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3% Acetic Acid with Methylene Blue

Reagent for manual counting of nucleated mammalian cells
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Trypan Blue

Reagent for counting viable mammalian cells

Buffers and Solutions 4 (4)

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Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum

Cell culture buffer
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EasySep™ Buffer

Cell separation buffer
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Ammonium Chloride Solution

Reagent for lysis of red blood cells
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ALDEFLUOR™ Assay Buffer

Additional assay buffer for use with the ALDEFLUOR™ kit

Primary and Cultured Cells 4 (2)

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Human Bone Marrow Stromal Cells, Frozen

Primary human cells, frozen
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Human Whole Bone Marrow, Fresh

Primary human cells, fresh

Cultureware and General Supplies 4 (2)

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Falcon® Round-Bottom Tubes with Cell Strainer Cap, 5 mL

Sterile, polystyrene, round-bottom tube with nylon mesh cell strainer snap cap
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Tissue Culture-Treated Dishes

Sterile tissue culture-treated dish; 100 mm format

Density Gradient Media 4 (1)

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Lymphoprep™

Density gradient medium for the isolation of mononuclear cells

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