AggreWell™ Microwell Plates - Spheroids and Embryoid Bodies

Easy, Reproducible 3D Spheroid and Embryoid Body Production

AggreWell™ plates provide a simple, user-friendly method to generate large numbers of highly uniform 3D spheroid cultures. Compatible with a wide range of cell types, AggreWell™ can be used for many applications, including directed differentiation of pluripotent stem cells (PSCs) using embryoid body (EB) protocols, as well as cancer research, drug discovery research, and suspension culture.

AggreWell™ plates contain microwells that are 400 µm (AggreWell™400) or 800 µm (AggreWell™800) in size, providing flexibility to generate spheroids of desired size for your research.

How Does AggreWell™ Work?

Watch this short video to learn more about how AggreWell™ works to generate uniformly-sized and shaped 3D spheroids and embryoid bodies.

AggreWell™ works by adding a single cell suspension to the wells containing microwells, and then centrifuging to distribute the cells evenly in the microwells. Incubate overnight and assess the spheroids after 24-48 hours. Then culture in the microwells, or harvest and perform downstream assays.

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Why Use AggreWell™ to Generate EBs and Spheroids?

  • Use a simple protocol to generate spheroids and EBs.
  • Produce large numbers of uniform spheroids, with consistent size and shape.
  • Control the size of the spheroids by modifying the cell seeding concentration.
  • Obtain up to 4,700 spheroids per well.
  • Scale up production of spheroids and EBs from a single plate for a fraction of a penny per spheroid.

AggreWell™ Products


Microwell Size:

400 µm microwells

Cell Range:

50 - 3,000 cells per spheroid

Plate Formats Available:

24-well plate
6-well plate


  • Embryoid body for ES and iPS cell directed differentiation
  • Cancer spheroid cultures
  • Drug discovery
  • 3D tissue engineering
  • Imaging spheroids


Microwell Size:

800 µm microwells

Cell Range:

3,000 - 20,000 cells per spheroid

Plate Formats Available:

24-well plate


  • Embryoid body for ES and iPS cell directed differentiation
  • Cancer spheroid cultures
  • Drug discovery
  • 3D tissue engineering
  • Imaging spheroids

AggreWell™ Rinsing Solution

Required for:

Preparation of AggreWell™ plates to remove bubbles from microwells and promote efficient spheroid formation.


  • Promotes efficient EB/spheroid formation
  • Prevents EB/spheroid adhesion
  • Easier to harvest EBs/Spheroids for downstream applications
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Scientific Resources

Key Applications and Related Publications

ES and iPS Cell Directed Differentiation (Selected)

Cheung et al. (2014) Telomerase protects werner syndrome lineage-specific stem cells from premature aging. Stem Cell Reports 2(4):534-46

Hartjes et al. (2014) Selection via pluripotency-related transcriptional screen minimizes the influence of somatic origin on iPSC differentiation propensity. Stem Cells 32(9):2350-9

Jang et al. (2014) Culture of Pig Induced Pluripotent Stem Cells without Direct Feeder Contact in Serum Free Media. J Stem Cell Res Ther 4(2):174

Kinney et al. (2014) Mesenchymal morphogenesis of embryonic stem cells dynamically modulates the biophysical microtissue niche. Sci Rep 4: 4290

Kokkinaki M. (2011) Human Induced Pluripotent Stem-Derived Retinal Pigment Epithelium (RPE) Cells Exhibit Ion Transport, Membrane Potential, Polarized Vascular Endothelial Growth Factor Secretion, and Gene Expression Pattern Similar to Native RPE. STEM CELLS 29:825–835

Nguyen et al. (2014) Microscale Generation of Cardiospheres Promotes Robust Enrichment of Cardiomyocytes Derived from Human Pluripotent Stem Cells. Stem Cell Reports 3(2): 260–268

Sebastiano V et al. (2014) Human COL7A1-corrected induced pluripotent stem cells for the treatment of recessive dystrophic epidermolysis bullosa. Sci Transl Med 6(264):264ra163

Ungrin et al. (2012) Rational bioprocess design for human pluripotent stem cell expansion and endoderm differentiation based on cellular dynamics. Biotechnol Bioeng 109(4): 853-66

van Wilgenburg et al. (2013) Efficient, long term production of monocyte-derived macrophages from human pluripotent stem cells under partly-defined and fully-defined conditions. PloS one 8(8):e71098

Disease Modeling

Aflaki et al. (2014) Macrophage models of Gaucher disease for evaluating disease pathogenesis and candidate drugs. Sci Transl Med. 11;6(240): 240ra73

Moya et al. (2013) An integrated model of perfused tumor and cardiac tissue. Stem Cell Research & Therapy, 4(Suppl 1):S15

3D Tissue Engineering

Suspension Culture of MSCs

Additional Applications

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