STEMdiff™
STEMdiff™ Differentiation Media for hPSCs
Reproducible Differentiation to Specialized Cell Types and Organoids
Human pluripotent stem cell (hPSC)-based models hold tremendous potential for the study of human development and disease. When working with hPSCs, even the most detailed and rigorously-followed protocols can still lead to inconsistent differentiation.1,2 Use STEMdiff™ to reproducibly differentiate across multiple human embryonic stem (ES) cell and induced pluripotent stem (iPS) cell lines. STEMdiff™ is part of the most complete system of reagents for human pluripotent stem cell (hPSC) culture - a system that includes mTeSR™1, the most published feeder-free ES cell and iPS cell culture medium. Media formulations, raw material specifications and manufacturing processes for all STEMdiff™ products have been carefully optimized in order to minimize reagent variability. In addition, STEMdiff™ products include detailed, user-friendly protocols to standardize your differentiation procedures.
Why Use STEMdiff™?
- Reduce variability between experiments.
- Use with hPSCs cultured with TeSR™ maintenance media.
- Optimized formulations and pre-screened components ensure reagent consistency and minimal lot-to-lot variability.
- Detailed protocols for differentiating to all three germ layers.
- Efficient differentiation of multiple human ES and iPS cell lines.
Which Lineages Are You Interested in Differentiating to?
Protocol Type:
Features/Advantages:
- Rapid neural induction using EB or monolayer protocols
- Serum-free* expansion and cryopreservation of neural progenitor cells
- Highly efficient generation of neuronal precursors from neural progenitor cells
- Maturation of precursors to functional forebrain-type neurons or cerebral organoids
Products:
Protocol Type:
Features/Advantages:
- Rapid mesodermal induction after only 2 - 4 days of differentiation
- Generate multipotent early mesoderm cells that can be further differentiated to multiple downstream mesodermal cell lineages including endothelial, osteogenic, chondrocytes and adipocytes
Products:
Protocol Type:
Features/Advantages:
- Generate multipotent definitive endoderm cells that can be differentiated to multiple downstream endodermal cell lineages including hepatic, pancreatic, intestinal and pulmonary
- Generate functional pancreatic progenitor cells that can be further differentiated to insulin-producing β-cells
Products:
Flexible User-Directed Differentiation
For customized differentiation protocols, explore a range of unsupplemented media that supports cell survival without lineage bias. Use to direct differentiation to a variety of cell lineages, including endothelial,3 smooth muscle,3 hematopoietic,4,5 skeletal muscle,6 and cardiomyocytes7.
Also See:
References
- D’Amour KA et al. (2005) Efficient differentiation of human embryonic stem cells to definitive endoderm. Nature Biotechnology 23(12):1534-41
- Kattman SJ et al. (2011) Stage-Specific Optimization of Activin/Nodal and BMP Signaling Promotes Cardiac Differentiation of Mouse and Human Pluripotent Stem Cell Lines. Cell Stem Cell 8(2): 228-240
- Tan JY et al. (2013) Efficient Derivation of Lateral Plate and Paraxial Mesoderm Subtypes from Human Embryonic Stem Cells Through GSKi-Mediated Differentiation. Stem Cells Dev 22(13): 1893-1906
- Protocol adapted from Chadwick et al. (2003) and Ng et al. (2008), and discussed in this Technical Bulletin
- Gertow et al. (2013) WNT3A Promotes Hematopoietic or Mesenchymal Differentiation from hESCs Depending on the Time of Exposure. Stem Cell Reports 1(1):53-65
- Xu C et al. (2013) A Zebrafish Embryo Culture System Defines Factors that Promote Vertebrate Myogenesis across Species. 155(4): 909-921
- Elliott DA et al. (2011) NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nat Methods 8(12):1037-1040