Human pluripotent stem cell (hPSC)-based models hold tremendous potential for the study of human development and disease. When working with hPSCs, even the most detailed and rigorously followed stem cell differentiation protocols can still lead to inconsistent differentiation.1,2 Use STEMdiff™ to reproducibly differentiate across multiple human embryonic stem (ES) cell and induced pluripotent stem (iPS) cell lines. STEMdiff™ is part of the most complete system of reagents for human pluripotent stem cell (hPSC) culture - a system that includes mTeSR™1, the most published feeder-free ES cell and iPS cell culture medium. Media formulations, raw material specifications and manufacturing processes for all STEMdiff™ products have been carefully optimized in order to minimize reagent variability. In addition, STEMdiff™ products include detailed, user-friendly protocols to standardize your differentiation procedures.

Why Use STEMdiff™?

  • Reduce variability between experiments.
  • Use with hPSCs cultured with TeSR™ maintenance media.
  • Optimized formulations and pre-screened components ensure reagent consistency and minimal lot-to-lot variability.
  • Detailed protocols for differentiating to all three germ layers.
  • Efficient differentiation of multiple human ES and iPS cell lines.

STEMdiff™ for ES and iPS Cell Differentiation to Endodermal Lineages

Definitive Endoderm

Lung Cells and Organoids

Pancreatic Progenitor Cells

Intestinal Organoids

STEMdiff™ for ES and iPS Cell Differentiation to Multiple Lineages

For customized differentiation protocols, explore a range of unsupplemented media that supports cell survival without lineage bias. Use to direct differentiation to a variety of cell lineages, including endothelial,3 smooth muscle,3 hematopoietic,4,5 skeletal muscle,6 and cardiomyocyte7.

Assessing Pluripotency by Trilineage Differentiation

Validate the ability of new or established human ES and iPS cell lines to differentiate to the three germ layers: ectoderm, mesoderm, and endoderm.

Find More Products for hPSC Differentiation

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