STEMdiff™

STEMdiff™ Pluripotent Stem Cell Differentiation Media

Reproducible Differentiation of Human ES and iPS Cells

Human pluripotent stem cell (hPSC)-based models hold tremendous potential for the study of human development and disease. When working with hPSCs, even the most detailed and rigorously followed stem cell differentiation protocols can still lead to inconsistent differentiation.^1,2 Use STEMdiff™ to reproducibly differentiate across multiple human embryonic stem (ES) cell and induced pluripotent stem (iPS) cell lines. STEMdiff™ is part of the most complete system of reagents for human pluripotent stem cell (hPSC) culture - a system that includes mTeSR™1, the most published feeder-free ES cell and iPS cell culture medium. Media formulations, raw material specifications and manufacturing processes for all STEMdiff™ products have been carefully optimized in order to minimize reagent variability. In addition, STEMdiff™ products include detailed, user-friendly protocols to standardize your differentiation procedures.

Why Use STEMdiff™?

DEFINED AND SERUM-FREE. Reduce variability between experiments.
COMPATIBLE. Use with hPSCs cultured with TeSR™ maintenance media.
REPRODUCIBLE. Optimized formulations and pre-screened components ensure reagent consistency and minimal lot-to-lot variability.
EASY-TO-USE. Detailed protocols for differentiating to all three germ layers.
MULTIPLE CELL LINES. Efficient differentiation of multiple human ES and iPS cell lines.

Which lineages are you differentiating hPSCs to? Ectoderm: Neurons, glial cells, brain organoids, and more. Mesoderm: Hematopoietic cells, immune cells, cardiomyocytes, and more. Endoderm: Intestinal organoids, lung progenitors, and more. Customizable differentiation to multiple lineages

Explore resources for hPSC differentiation, including videos and webinars, technical tips and protocols, and more.

STEMdiff™ for ES and iPS Cell Differentiation to Ectodermal Lineages

Neurons, Glial Cells, and Brain Organoids

(See below for Microglia differentiation)

STEMdiff™ for ES and iPS Cell Differentiation to Mesodermal Lineages

Early Mesodermal Cells

Mesoderm Induction Medium

Hematopoietic Cells and Immune Cells

Cardiomyocytes

Endothelial Cells

Other

STEMdiff™ for ES and iPS Cell Differentiation to Endodermal Lineages

Definitive Endoderm

Lung and Pancreatic Progenitor Cells

Intestinal Organoids

Intestinal Organoid Kit

STEMdiff™ for ES and iPS Cell Differentiation to Multiple Lineages

For customized differentiation protocols, explore a range of unsupplemented media that supports cell survival without lineage bias. Use to direct differentiation to a variety of cell lineages, including endothelial,³ smooth muscle,³ hematopoietic,^4,5 skeletal muscle,⁶ and cardiomyocyte⁷.

Assessing Pluripotency by Trilineage Differentiation

Validate the ability of new or established human ES and iPS cell lines to differentiate to the three germ layers: ectoderm, mesoderm, and endoderm.

STEMdiff™ Trilineage Differentiation Kit

Find More Products for hPSC Differentiation

Copyright © 2020 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, AGGREWELL, CELLADHERE, CLONACELL, EASYPLATE, EASYSEP, ENDOCULT, EPICULT, ESCULT, ERYTHROCLEAR, FRESR, GLOCELL, MAMMOCULT, MEGACULT, MESENCULT, METHOCULT, MYELOCULT, NEUROCULT, PNEUMACULT, PROSTACULT, RELESR, ROBOSEP, ROSETTESEP, SEPMATE, SMARTDISH, SPINSEP, STEMDIFF, STEMSEP, STEMSPAN, and STEMVISION are trademarks of STEMCELL Technologies Canada Inc. All other trademarks and registered trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.