Overview
- MesenCult™ MSC Basal Medium (Human), 450 mL (Catalog #05401)
- MesenCult™ MSC Stimulatory Supplement (Human), 50 mL (Catalog #05402)
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(8)Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Human Mesenchymal Stem and Progenitor cells (MSCs) Derived and Culture-Expanded Using the MesenCult™ Proliferation Kit
(A) Human bone marrow mesenchymal stem and progenitor cells (BM MSCs) were derived and culture-expanded for 8 passages in complete MesenCult™ Proliferation Medium (n = 6) and two commercially available media (Commercial Medium 1 and Commercial Medium 2; n = 3). (B) The average doubling time in days over 8 passages of human BM MSCs culture-expanded in complete MesenCult™ Proliferation Medium, and Commercial Medium 1 and 2. Vertical lines indicate standard error of the mean.

Figure 2. Human MSCs Culture-Expanded in Complete MesenCult™ Proliferation Medium Retain Strong Multi-Lineage Differentiation Potential
(A) Human BM-derived MSCs cultured in complete MesenCult™ Proliferation Medium differentiate into (B) adipocytes (Oil Red O staining), (C) chondrocytes (Alcian Blue staining) and (D) osteoblasts (alkaline phosphatase and von Kossa staining).

Figure 3. Flow Cytometric Analysis of Culture-Expanded Human MSCs in Complete MesenCult™ Proliferation Medium
Human BM-derived MSCs from passage 5 were stained for mesenchymal cell surface markers CD73, CD90 and CD105, and the hematopoietic markers CD34 and CD45 (shown in grey shaded areas). BM-derived MSCs are positive for MSC markers and negative for hematopoietic markers. High expression of the endothelial marker, CD146, was also observed in BM-derived MSCs (shown in grey shaded areas). Isotype control is shown by red line.