Top Applications in Human ES Cell and iPS Cell Research

As part of the Pluripotent Profiles series, STEMCELL Technologies has selected influential publications that have supported the following applications of human ES cells and iPS cells. View our lists of top five publications, and read the interviews to learn what some of these researchers have said about their work.

Key Applications

Toxicity Testing with Human iPS Cells

Jagtap S, Meganathan K, Gaspar J, Wagh V, Winkler J, Hescheler J and Sachinidis A (2011), Cytosine arabinoside induces ectoderm and inhibits mesoderm expression in human embryonic stem cells during multilineage differentiation, Br J Pharmacol. Vol. 162, pp. 1743-56.
Kleinstreuer NC, Smith AM, West PR, Conard KR, Fontaine BR, Weir-Hauptman AM, Palmer JA, Knudsen TB, Dix DJ, Donley ELR and Cezar GG (2011), Identifying developmental toxicity pathways for a subset of ToxCast chemicals using human embryonic stem cells and metabolomics, Toxicology and Applied Pharmacology., November, 2011. Vol. 257(1), pp. 111-121.
Liang P, Lan F, Lee AS, Gong T, Sanchez-Freire V, Wang Y, Diecke S, Sallam K, Knowles JW, Wang PJ, Nguyen PK, Bers DM, Robbins RC and Wu JC (2013), Drug screening using a library of human induced pluripotent stem cell-derived cardiomyocytes reveals disease-specific patterns of cardiotoxicity. Circulation., April, 2013. Vol. 127(16), pp. 1677-1691.
Liu J, Sun N, Bruce MA, Wu JC and Butte MJ (2012), Atomic Force Mechanobiology of Pluripotent Stem Cell-Derived Cardiomyocytes, PLoS ONE., May, 2012. Vol. 7(5), pp. e37559.
Mehta A, Chung YY, Ng A, Iskandar F, Atan S, Wei H, Dusting G, Sun W, Wong P and Shim W (2011), Pharmacological response of human cardiomyocytes derived from virus-free induced pluripotent stem cells, Cardiovascular Research., September, 2011. Vol. 91(4), pp. 577-586.

Differentiating to Hematopoietic Cells

Brown ME, Rondon E, Rajesh D, Mack A, Lewis R, Feng X, Zitur LJ, Learish RD and Nuwaysir EF (2010), Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes, PLoS One. Vol. 5, pp. e11373.
Carpenter L, Malladi R, Yang C-T, French A, Pilkington KJ, Forsey RW, Sloane-Stanley J, Silk KM, Davies TJ, Fairchild PJ, Enver T and Watt SM (2011), Human induced pluripotent stem cells are capable of B-cell lymphopoiesis, Blood., April, 2011. Vol. 117(15), pp. 4008-4011.
Dravid G, Zhu Y, Scholes J, Evseenko D and Crooks GM (2011), Dysregulated gene expression during hematopoietic differentiation from human embryonic stem cells, Mol Ther. Vol. 19, pp. 768-81.
Niwa A, Heike T, Umeda K, Oshima K, Kato I, Sakai H, Suemori H, Nakahata T and Saito MK (2011), A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors, PLoS One. Vol. 6, pp. e22261.
Salvagiotto G, Burton S, Daigh CA, Rajesh D, Slukvin II and Seay NJ (2011), A Defined, Feeder-Free, Serum-Free System to Generate In Vitro Hematopoietic Progenitors and Differentiated Blood Cells from hESCs and hiPSCs, PLoS ONE., March, 2011. Vol. 6(3), pp. e17829.

Differentiating to Definitive Endoderm

Hannoun Z, Fletcher J, Greenhough S, Medine C, Samuel K, Sharma R, Pryde A, Black JR, Ross JA, Wilmut I, Iredale JP and Hay DC (2010), The comparison between conditioned media and serum-free media in human embryonic stem cell culture and differentiation, Cell Reprogram. Vol. 12, pp. 133-40.
Jaramillo M and Banerjee I (2012), Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach, Journal of Visualized Experiments., March, 2012. (61)
Miki T, Ring A and Gerlach J (2011), Hepatic differentiation of human embryonic stem cells is promoted by three-dimensional dynamic perfusion culture conditions, Tissue Eng Part C Methods. Vol. 17, pp. 557-68.
Mou H, Zhao R, Sherwood R, Ahfeldt T, Lapey A, Wain J, Sicilian L, Izvolsky K, Lau FH, Musunuru K, Cowan C and Rajagopal J (2012), Generation of Multipotent Lung and Airway Progenitors from Mouse ESCs and Patient-Specific Cystic Fibrosis iPSCs, Cell Stem Cell., April, 2012. Vol. 10(4), pp. 385-397.
Spence JR, Mayhew CN, Rankin SA, Kuhar MF, Vallance JE, Tolle K, Hoskins EE, Kalinichenko VV, Wells SI, Zorn AM, Shroyer NF and Wells JM (2011), Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro, Nature., February, 2011. Vol. 470(7332), pp. 105-109.

Scale-Up and Bioreactor Culture

Krawetz R, Taiani JT, Liu S, Meng G, Li X, Kallos MS and Rancourt DE (2010), Large-Scale Expansion of Pluripotent Human Embryonic Stem Cells in Stirred-Suspension Bioreactors, Tissue Engineering Part C: Methods., August, 2010. Vol. 16(4), pp. 573-582.
Oh SKW, Chen AK, Mok Y, Chen X, Lim U-M, Chin A, Choo ABH and Reuveny S (2009), Long-term microcarrier suspension cultures of human embryonic stem cells, Stem Cell Research., May, 2009. Vol. 2(3), pp. 219-230.
Olmer R, Haase A, Merkert S, Cui W, Palecek J, Ran C, Kirschning A, Scheper T, Glage S, Miller K, Curnow EC, Hayes ES and Martin U (2010), Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium, Stem Cell Res. Vol. 5, pp. 51-64.
Singh H, Mok P, Balakrishnan T, Rahmat SN and Zweigerdt R (2010), Up-scaling single cell-inoculated suspension culture of human embryonic stem cells, Stem Cell Res. Vol. 4, pp. 165-79.
Zweigerdt R, Olmer R, Singh H, Haverich A and Martin U (2011), Scalable expansion of human pluripotent stem cells in suspension culture, Nature Protocols. Vol. 6(5), pp. 689-700.

Differentiating to Cardiomyocytes

Elliott DA, Braam SR, Koutsis K, Ng ES, Jenny R, Lagerqvist EL, Biben C, Hatzistavrou T, Hirst CE, Yu QC, Skelton RJ, Ward-van Oostwaard D, Lim SM, Khammy O, Li X, Hawes SM, Davis RP, Goulburn AL, Passier R, Prall OW, Haynes JM, Pouton CW, Kaye DM, Mummery CL, Elefanty AG and Stanley EG (2011), NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes, Nat Methods. Vol. 8, pp. 1037-40.
Hazeltine LB, Simmons CS, Salick MR, Lian X, Badur MG, Han W, Delgado SM, Wakatsuki T, Crone WC, Pruitt BL and Palecek SP (2012), Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells, International Journal of Cell Biology. Vol. 2012, pp. 1-13.
Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK, Zhang J, Kamp TJ and Palecek SP (2012), Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling, Proceedings of the National Academy of Sciences., July, 2012. Vol. 109(27), pp. 10759-10760.
Mehta A, Chung YY, Ng A, Iskandar F, Atan S, Wei H, Dusting G, Sun W, Wong P and Shim W (2011), Pharmacological response of human cardiomyocytes derived from virus-free induced pluripotent stem cells, Cardiovascular Research., September, 2011. Vol. 91(4), pp. 577-586.
Zhang H, Zou B, Yu H, Moretti A, Wang X, Yan W, Babcock JJ, Bellin M, McManus OB, Tomaselli G, Nan F, Laugwitz K-L and Li M (2012), Modulation of hERG potassium channel gating normalizes action potential duration prolonged by dysfunctional KCNQ1 potassium channel, Proceedings of the National Academy of Sciences., July, 2012. Vol. 109(29), pp. 11866-11871.

Most of the ground-breaking research profiled here was supported by mTeSR™1, the most widely published cell culture medium for the feeder-free maintenance of ES cells and iPS cells. A highly specialized, serum-free and complete cell culture medium, mTeSR™1 has been used in over 40 countries to successfully maintain hundreds of ES cell and iPS cell lines.