Defined, feeder-free maintenance medium for human ES and iPS cells

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Defined, feeder-free maintenance medium for human ES and iPS cells
From: 272 USD



mTeSR™1 is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation. It has been used to successfully maintain hundreds of ES and iPS cell lines in over 40 countries, and has supported top pluripotent stem cell publications and researchers. mTeSR™1 is a highly specialized, serum-free, and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR™1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.
  • mTeSR™1 Complete Kit (Catalog #05850)
    • mTeSR™1 Basal Medium, 400 mL
    • mTeSR™1 5X Supplement, 100 mL
  • mTeSR™1 Complete Kit, 1 L (Catalog #05857)
    • mTeSR™1 Basal Medium, 800 mL
    • mTeSR™1 5X Supplement, 100 mL, 2 Bottles
  • mTeSR™1 Complete Kit, 10 Pack (Catalog #05870)
    • mTeSR™1 Basal Medium, 400 mL, 10 Bottles
    • mTeSR™1 5X Supplement, 100 mL, 10 Bottles
  • mTeSR™1 Complete Kit, 25 Pack (Catalog #05875)
    • mTeSR™1 Basal Medium, 400 mL, 25 Bottles
    • mTeSR™1 5X Supplement, 100 mL, 25 Bottles
Specialized Media
Cell Type:
Pluripotent Stem Cells
Maintenance; Expansion; Cell Culture
Area of Interest:
Stem Cell Biology
Serum-Free; Defined

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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications


Morphology of hESCs and hiPSCs Cultured in mTeSR™1

Figure 1. Morphology of hESCs and hiPSCs Cultured in mTeSR™1

H1 hESCs grow as colonies with (A) defined edges and (B) high nucleus-to-cytoplasm ratio. hiPSC lines (C) iPSC(IMR90)-3 and (D) MSC-iPSC1 maintained in mTeSR™1 show similar morphological characteristics. hiPSC photographs courtesy of M. O'Connor and C. Eaves, The Vancouver Human Embryonic Stem Cell Core Facility.

Human Embryonic Stem Cells Cultured in mTeSR™1 Retain Normal Karyotype Following Long-Term Passage

Figure 2. Human Embryonic Stem Cells Cultured in mTeSR™1 Retain Normal Karyotype Following Long-Term Passage

Chromosomal analysis of H1 hESCs cultured in mTeSR™1 for 48 passages shows that normal karyotype is retained during long-term passaging. Data from Dr. T Ludwig, WiCell Research Institute.

Human Embryonic Stem Cell Cultures in mTeSR™1 Are Pluripotent

Figure 3. Human Embryonic Stem Cell Cultures in mTeSR™1 Are Pluripotent

H9 hESCs were cultured for 6 passages in mTeSR™1 then injected subcutaneously into immunocompromised mice. The resulting teratoma contained cell types from all 3 germ layers. Representative tissue types are shown.

Human Embryonic Stem Cells Cultured in mTeSR™1 Express High Levels of Pluripotent Markers and Low Levels of Differentiation Markers

Figure 4. Human Embryonic Stem Cells Cultured in mTeSR™1 Express High Levels of Pluripotent Markers and Low Levels of Differentiation Markers

Flow cytometric analysis of H9 hESCs maintained in mTeSR™1 for 17 passages.


Pathobiology : journal of immunopathology, molecular and cellular biology 2017

Overexpression of KIF11 in Gastric Cancer with Intestinal Mucin Phenotype.

Imai T et al.


OBJECTIVE Gastric cancer (GC) is one of the most common human cancers. A useful method of gastric cancer stem cell (CSC) characterization is spheroid colony formation. Previously, we reported that KIF11 expression is textgreater2-fold in spheroid-body-forming GC cells compared with parental cells. Here, we analyzed the expression and distribution of KIF11 in human GC by immunohistochemistry. METHODS Expression of KIF11 in 165 GC cases was determined using immunohistochemistry. For mucin phenotypic expression analysis of GC, immunostaining of MUC5AC, MUC6, MUC2 and CD10 was evaluated. RNA interference was used to inhibit KIF11 expression in GC cell lines. RESULTS In total, 119 of 165 GC cases (72%) were positive for KIF11. Expression of KIF11 was not associated with any clinicopathologic characteristics; however, it was observed frequently in GC exhibiting an intestinal phenotype. Both the number and size of spheres formed by MKN-74 cells were significantly reduced following transfection of KIF11-targeting siRNA compared with negative-control siRNA. Furthermore, levels of phosphorylated Erk1/2 were lower in KIF11 siRNA-transfected cells than with negative-control siRNA-transfected cells. CONCLUSION These results indicate that KIF11 is involved in intestinal mucin phenotype GC.
Stem cell reports 2016 SEP

Human iPSC-Derived Neuronal Model of Tau-A152T Frontotemporal Dementia Reveals Tau-Mediated Mechanisms of Neuronal Vulnerability.

Silva MC et al.


Frontotemporal dementia (FTD) and other tauopathies characterized by focal brain neurodegeneration and pathological accumulation of proteins are commonly associated with tau mutations. However, the mechanism of neuronal loss is not fully understood. To identify molecular events associated with tauopathy, we studied induced pluripotent stem cell (iPSC)-derived neurons from individuals carrying the tau-A152T variant. We highlight the potential of in-depth phenotyping of human neuronal cell models for pre-clinical studies and identification of modulators of endogenous tau toxicity. Through a panel of biochemical and cellular assays, A152T neurons showed accumulation, redistribution, and decreased solubility of tau. Upregulation of tau was coupled to enhanced stress-inducible markers and cell vulnerability to proteotoxic, excitotoxic, and mitochondrial stressors, which was rescued upon CRISPR/Cas9-mediated targeting of tau or by pharmacological activation of autophagy. Our findings unmask tau-mediated perturbations of specific pathways associated with neuronal vulnerability, revealing potential early disease biomarkers and therapeutic targets for FTD and other tauopathies.
Stem cells and development 2016 SEP

Induced Pluripotent Stem Cell Differentiation and Three-Dimensional Tissue Formation Attenuate Clonal Epigenetic Differences in Trichohyalin.

Petrova A et al.


The epigenetic background of pluripotent stem cells can influence transcriptional and functional behavior. Most of these data have been obtained in standard monolayer cell culture systems. In this study, we used exome sequencing, array comparative genomic hybridization (CGH), miRNA array, DNA methylation array, three-dimensional (3D) tissue engineering, and immunostaining to conduct a comparative analysis of two induced pluripotent stem cell (iPSC) lines used in engineering of 3D human epidermal equivalent (HEE), which more closely approximates epidermis. Exome sequencing and array CGH suggested that their genome was stable following 3 months of feeder-free culture. While the miRNAome was also not affected, ≈7% of CpG sites were differently methylated between the two lines. Analysis of the epidermal differentiation complex, a region on chromosome 1 that contains multiple genes involved in skin barrier maturation (including trichohyalin, TCHH), found that in one of the iPSC clones (iKCL004), TCHH retained a DNA methylation signature characteristic of the original somatic cells, whereas in other iPSC line (iKCL011), the TCHH methylation signature matched that of the human embryonic stem cell line KCL034. The difference between the two iPSC clones in TCHH methylation did not have an obvious effect on its expression in 3D HEE, suggesting that differentiation and tissue formation may mitigate variations in the iPSC methylome.
Neurotoxicology 2016 SEP

Silver nanoparticles exhibit coating and dose-dependent neurotoxicity in glutamatergic neurons derived from human embryonic stem cells.

Begum AN et al.


Silver nanoparticles (AgNPs) are used extensively as anti-microbial agents in various products, but little is known about their potential neurotoxic effects. In this study, we used glutamatergic neurons derived from human embryonic stem cells as a cellular model to study 20nm citrate-coated AgNPs (AgSCs) and Polyvinylpyrrolidone-coated AgNPs (AgSPs) induced neurotoxicity. AgSCs significantly damaged neurite outgrowths; increased the production of reactive oxygen species and Ca(2+) influxes; reduced the expression of MAP2, PSD95, vGlut1 and NMDA receptor proteins at concentrations as low as 0.1$g/ml. In contrast, AgSPs exhibited neurotoxicity only at higher concentration. Furthermore, our results showed that AgSCs induced glutamate excitotoxicity by the activation of calmodulin and the induction of nitric oxide synthase; increased the phosphorylation of glycogen synthase kinase-3 $/$ at Tyr(216) and Tau at Ser(396) and reduced the expression of Tau46, which are typically observed in Alzheimer's disease. This study indicated that stem cells can provide an excellent platform for studying nanoparticle induced neurotoxicity.
Bioprocess and biosystems engineering 2016 SEP

Culture medium refinement by dialysis for the expansion of human induced pluripotent stem cells in suspension culture.

Nath SC et al.


Human induced pluripotent stem cells (hiPSCs) secrete essential autocrine factors that are removed along with toxic metabolites when the growth medium is exchanged daily. In this study, after determining the minimum inhibitory level of lactic acid for hiPSCs, a medium refining system was constructed by which toxic metabolites were removed from used culture medium and autocrine factors as well as other growth factors were recycled. Specifically, about 87 % of the basic fibroblast growth factor and 80 % of transforming growth factor beta 1 were retained in the refined medium after dialysis. The refined medium efficiently potentiated the proliferation of hiPS cells in adherent culture. When the refining system was used to refresh medium in suspension culture, a final cell density of (1.1 ± 0.1) × 10(6) cells mL(-1) was obtained, with 99.5 ± 0.2 % OCT 3/4 and 78.3 ± 1.1 % TRA-1-60 expression, on day 4 of culture. These levels of expression were similar to those observed in the conventional suspension culture. With this method, culture medium refinement by dialysis was established to remove toxic metabolites, recycle autocrine factors as well as other growth factors, and reduce the use of macromolecules for the expansion of hiPSCs in suspension culture.
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