Register: On-Demand PSC Course
Register now to complete a virtual training course on pluripotent stem cell quality and maintenance.
Learn the key concepts of human pluripotent stem cell (hPSC) biology, hPSC characterization, and maintenance of hPSCs.
Instructior Bio
Jane Savill - Manager, Scientific Support, Pluripotent Stem Cell Biology
Jane Savill is a Scientific Manager in the Scientific Support Department at STEMCELL Technologies. Jane obtained her PhD in Oncology from Cambridge University and before joining STEMCELL, she worked as a Product Development Scientist for a small start-up focused on Rheumatoid Arthritis diagnostics. At STEMCELL, Jane manages frontline support with our product portfolios to the global customer base and sales staff. She specializes in STEMCELL’s hPSC product portfolio and overseas training in corporate and client training programs with a focus on hPSC products and protocols.
- Recognize the difference between the functions of stem cells and non-stem cells
- Describe the types of stem cells based on potency or developmental origin
- Define pluripotent stem cells and differences between embryonic and adult stem cells
- Know where to obtain hPSCs for your research
Mini Quiz: Test Your Knowledge
Estimated time for completion: 12 minutes
- Understand the importance of maintaining high-quality human pluripotent stem cells (hPSCs)
- Recognize how the ISSCR Standards for Human Stem Cell Use in Research document can be applied to your own research
- Recall the characteristics of hPSCs and how to assess these attributes in terms of basic characterization, pluripotency and the undifferentiated state, and genomic integrity
Mini Quiz: Test Your Knowledge
Estimated time for completion: 43 minutes
- Summarize the progression of hPSC culture conditions over time
- Compare and contrast different matrices concerning:
- Composition
- Preparation
- Plate coating
Mini Quiz: Test Your Knowledge
Estimated time for completion: 13 minutes
- Describe how media can be defined as serum-free, animal component-free, animal origin-free, and xeno-free, and how this relates to manufacturing processes
- Understand what requirements researchers need for the maintenance of human pluripotent stem cells (hPSCs)
- Be aware of hPSCs maintenance media options offered by STEMCELL
Mini Quiz: Test Your Knowledge
Estimated time for completion: 18 minutes
- Understand the process of passaging human pluripotent stem cells (hPSCs) and when is best to passage your hPSCs
- Be aware of hPSC dissociation reagents offered by STEMCELL
Mini Quiz: Test Your Knowledge
Estimated time for completion: 11 minutes
- Discuss the principles of cryopreservation and thawing
- Understand when to freeze down hPSC as single cells or aggregates
- Be aware of hPSC cryopreservation reagents offered by STEMCELL
Mini Quiz: Test Your Knowledge
Estimated time for completion: 7 minutes
Complete this final assessment to test your knowledge and receive a certificate following successful completion.
Estimated time for completion: 15 minutes
