Human Pluripotent Stem Cell Research

Maintenance of high quality human embryonic stem (ES) and induced pluripotent stem (iPS) cells is critical to success in all applications of PSC research. See MoreSpecific culture conditions are required in order to maintain undifferentiated ES and iPS cells that are capable of self-renewal. Use our defined, feeder-free TeSR-based media , xeno-free cell attachment substrates, and animal component-free passaging reagents, to culture hPSCs and minimize variation in your research. For long-term storage, our suite of cryopreservation media is designed to maintain high viability and maximize hPSC recovery after thawing. For help with your hPSC workflow decision making, use our infographics to find the right reagents for you.

Learn More

Technical Bulletin Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Tech Tip Coating Plates with Matrigel™ for Pluripotent Stem Cell Culture
Tech Tip Tap Your Way to Easy Pluripotent Stem Cell Passaging Using ReLeSR™
Wallchart Stem Cell States: Naive to Primed Pluripotency
Webinar (3:11) Choosing the Right Medium for Your hPSC Culture

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Products

Cell Culture Media and Supplements (4 13)

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CloneR™2

Defined supplement for improving survival of human ES and iPS cells in single-cell workflows
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TeSR™-AOF

cGMP, animal origin-free, stabilized, feeder-free maintenance medium for human ES and iPS cells
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mTeSR™ Plus

cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells
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TeSR™-E8™3D

Animal component-free suspension culture medium for human ES and iPS cells

Matrices and Substrates (4 3)

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Vitronectin XF™

Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
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CellAdhere™ Laminin-521

Matrix for maintenance of human ES and iPS cells in combination with TeSR™ maintenance media
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0.1% Gelatin in Water

Coating for cultureware

Tissue and Cell Culture Dissociation Reagents (4 5)

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Dispase, ACF

Animal component-free non-specific protease
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ReLeSR™

cGMP, enzyme-free human pluripotent stem cell selection and passaging reagent
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Dispase (1 U/mL)

1 U/mL dispase in DMEM/F-12
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Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent

Cell Storage Media (4 6)

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CryoStor® CS5

Animal component-free, defined cryopreservation medium with 5% DMSO
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CryoStor® CSB

Animal component-free and protein-free cryopreservation formulation
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mFreSR™

Serum-free cryopreservation medium for human ES and iPS cells
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FreSR™-S

Animal component-free medium for cryopreserving ES and iPS cells as single cells

Antibodies (4 7)

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Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20

Mouse monoclonal IgG2b antibody against human OCT4 (OCT3)
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Anti-Human SSEA-4 Antibody, Clone MC-813-70

Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4
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Anti-Human SSEA-5 Antibody, Clone 8e11

Mouse monoclonal IgG1 antibody against human SSEA-5
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Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R

Mouse monoclonal IgM antibody against human, rhesus, rabbit TRA-1-60 (podocalyxin)

Small Molecules (4 24)

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1-Azakenpaullone

Inhibits glycogen synthase kinase 3β (GSK3β)
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MLN4924

Inhibits NEDD8-activating enzyme
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NU7026

NHEJ pathway inhibitor; Inhibits DNA-dependent protein kinase (DNA-PK)
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FK506

Calcineurin/NFAT pathway inhibitor; Binds FKBP-12

Cytokines and Proteins (4 12)

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Human Recombinant TGF-beta 1 (Cell Culture Ready)

Ready-to-use transforming growth factor beta 1
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Human Recombinant PDGF-DD

Platelet-derived growth factor DD
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Mouse Recombinant IGF-I

Insulin-like growth factor 1
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Human/Mouse Recombinant Activin A, ACF

Activin A, animal component-free

Cultureware and General Supplies (4 4)

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Axygen® Thin-Wall PCR Tubes

Non-sterile, clear, RNase/DNase-free, polypropylene conical tubes with caps
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Costar® Microcentrifuge Tubes

Non-sterile, clear, polypropylene snap-cap microcentifuge tubes
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Reversible Strainers

Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids
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96-Well Treated Tissue Culture Plate

96-Well treated tissue culture plates

Buffers and Solutions (4 1)

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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

Training and Education (4 3)

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Reprogramming Human Somatic Cells to Induced Pluripotent Stem Cells

Learn how to derive and characterize human induced pluripotent stem (iPS) cells from somatic cells through live virtual training.
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Pluripotent Stem Cell Maintenance & Cell Quality

Learn how to effectively maintain and characterize high-quality human pluripotent stem cells (hPSCs) through live virtual training
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Pluripotent Stem Cell Training

Training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors

Primary and Cultured Cells (4 1)

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Healthy Control Human iPSC Line, Female, SCTi003-A

Human pluripotent stem cell line, frozen

Cell Engineering and Molecular Tools (4 12)

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ArciTect™ Human CRISPR Optimization Kit

Complete kit for optimization of genome editing in human cells
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ArciTect™ Cas9-eGFP Nuclease

Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing
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ArciTect™ sgRNA

Custom-designed single guide RNA for CRISPR-Cas9 genome editing
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ArciTect™ T7 Endonuclease I Kit

For estimation of CRISPR-Cas9 genome editing efficiency

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