Human Pluripotent Stem Cell Research

Maintenance of high quality human embryonic stem (ES) and induced pluripotent stem (iPS) cells is critical to success in all applications of PSC research. See MoreSpecific culture conditions are required in order to maintain undifferentiated ES and iPS cells that are capable of self-renewal. Use our defined, feeder-free TeSR-based media , xeno-free cell attachment substrates, and animal component-free passaging reagents, to culture hPSCs and minimize variation in your research. For long-term storage, our suite of cryopreservation media is designed to maintain high viability and maximize hPSC recovery after thawing. For help with your hPSC workflow decision making, use our infographics to find the right reagents for you.

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Technical Bulletin Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Tech Tip Coating Plates with Matrigel™ for Pluripotent Stem Cell Culture
Tech Tip Tap Your Way to Easy Pluripotent Stem Cell Passaging Using ReLeSR™
Wallchart Stem Cell States: Naive to Primed Pluripotency
Webinar (3:11) Choosing the Right Medium for Your hPSC Culture

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Products

Cell Culture Media and Supplements 4 (13)

eTeSR™

Stabilized, feeder-free medium for single-cell passaging of human pluripotent stem cells
TeSR™-AOF

cGMP, animal origin-free, stabilized, feeder-free maintenance medium for human ES and iPS cells
RSeT™ Feeder-Free Medium

Serum-free medium for naïve-like human pluripotent stem cells
NaïveCult™ Expansion Medium

Defined cell culture medium for expansion of reset naïve human ES and iPS cells

Matrices and Substrates 4 (4)

0.1% Gelatin in Water

Coating for cultureware
CellAdhere™ Dilution Buffer

Dilution buffer for matrix proteins, e.g. Vitronectin XF™
Vitronectin XF™

Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
CellAdhere™ Laminin-521

Matrix for maintenance of human ES and iPS cells in combination with TeSR™ maintenance media

Tissue and Cell Culture Dissociation Reagents 4 (5)

Collagenase Type IV (1 mg/mL)

Cell dissociation reagent
Gentle Cell Dissociation Reagent

cGMP, enzyme-free cell dissociation reagent
Dispase (1 U/mL)

1 U/mL dispase in DMEM/F-12
Dispase, ACF

Animal component-free non-specific protease

Cell Storage Media 4 (6)

CryoStor® CS2

Animal component-free, defined cryopreservation medium with 2% DMSO
FreSR™-S

Animal component-free medium for cryopreserving ES and iPS cells as single cells
CryoStor® CS5

Animal component-free, defined cryopreservation medium with 5% DMSO
CryoStor® CSB

Animal component-free and protein-free cryopreservation formulation

Antibodies 4 (7)

Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-54 (tissue non-specific alkaline phosphatase)
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-49 (tissue non-specific alkaline phosphatase)
Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20

Mouse monoclonal IgG2b antibody against human OCT4 (OCT3)
Anti-Human SSEA-4 Antibody, Clone MC-813-70

Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4

Small Molecules 4 (24)

1-Azakenpaullone

Inhibits glycogen synthase kinase 3β (GSK3β)
RSC-133

Epigenetic modifier; Inhibits DNA methyltransferase (DNMT)
Gatifloxacin

Antibiotic; Inhibits bacterial DNA gyrase and topoisomerase IV
ID-8

DYRK pathway inhibitor

Cytokines and Proteins 4 (16)

Human Recombinant TGF-beta 1 (Cell Culture Ready)

Ready-to-use transforming growth factor beta 1
Human Recombinant IGF-I LR3, ACF

Insulin-like growth factor 1 long arginine 3, animal component-free
Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF

Follistatin-resistant Activin A, animal component-free
Human Recombinant TGF-beta 1, ACF

Transforming growth factor beta 1, animal component-free

Cultureware and General Supplies 4 (3)

Axygen® Thin-Wall PCR Tubes

Non-sterile, clear, RNase/DNase-free, polypropylene conical tubes with caps
Costar® Microcentrifuge Tubes

Non-sterile, clear, polypropylene snap-cap microcentrifuge tubes
Reversible Strainers

Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

Laboratory Equipment 4 (2)

Hausser Scientific™ Hemocytometer Cover Glasses

Replacement cover glasses for use with Hausser Scientific™ Hemocytometer
Hausser Scientific™ Bright-Line Hemocytometer

Counting chamber with 2 cover glasses

Buffers and Solutions 4 (1)

D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

Cell Engineering and Molecular Tools 4 (13)

ArciTect™ High-Fidelity DNA Polymerase Kit

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification
ArciTect™ Cas9-eGFP Nuclease

Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing
ArciTect™ sgRNA

Custom-designed single guide RNA for CRISPR-Cas9 genome editing
ArciTect™ Annealing Buffer (5X)

For guide RNA generation in CRISPR-Cas9 genome editing

Primary and Cultured Cells 4 (3)

Healthy Control Human iPSC Line, Male, SCTi004-A

Human pluripotent stem cell line, frozen
Healthy Control Human iPSC Line, Female, SCTi003-A

Human pluripotent stem cell line, frozen
Healthy Control Human iPSC Line, Male, SCTi006-A

Human pluripotent stem cell line, frozen

Training and Education 4 (4)

Live Virtual Training: PSC Quality & Maintenance

Learn to culture, maintain, and assess your human pluripotent stem cells (hPSCs) in a feeder-free system.
Live Virtual Training: Reprogramming Somatic Cells to iPSCs

Acquire skills in generating, characterizing, and banking induced pluripotent stem cells (iPSCs) from somatic cells.
Live Virtual Training: Genome-Editing Pluripotent Stem Cells

Learn best practices for deriving and maintaining CRISPR-Cas9 genome-edited hPSCs.
Pluripotent Stem Cell Training

Training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors

Instruments and Software 4 (3)

PBS-MINI MagDrive Bioreactor

Compact bioreactor with single-use vessels for high-throughput, 3D suspension culture of hPSCs and other cell types
PBS-MINI Single-Use Vessel with Bottom Port

Single-use vessels with bottom port
Extended 4-Gang Power Distribution Cable

Four-way split cable to connect up to four PBS-MINI MagDrive Bioreactor Base Units to one power source