Tap Your Way to Easy Pluripotent Stem Cell Passaging Using ReLeSR™
Technical tip from our dedicated team of Product and Scientific Support specialists
Passaging human embryonic and induced pluripotent stem cells is now easier. ReLeSR™ is an enzyme-free reagent for dissociation and passaging of human pluripotent stem cells (hPSCs) as aggregates without manual selection or scraping.
Some of the critical aspects for successful generation of optimally-sized aggregates are outlined in the points below:
Some of the critical aspects for successful generation of optimally-sized aggregates are outlined in the points below:
- When adding ReLeSR™ to the cells, add and aspirate within one minute, and then incubate according to the protocol. This ensures that cells are exposed only to a residual film of ReLeSR™.
- Incubation time and temperature can be different for various cell lines cultured in different conditions, and therefore may need optimization. Please see Notes and Tips section in the Product Information Sheet for ReLeSR™.
- When tapping, it is important to be gentle but firm. For a demonstration of the recommended tapping technique, please see the video below.
- After tapping, pipette up and down only once, to ensure that the aggregates are uniform and the ideal size for passaging. Additional pipetting or other manipulations are not required.
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