FreSR™-S

Animal component-free medium for cryopreserving ES and iPS cells as single cells

FreSR™-S

Animal component-free medium for cryopreserving ES and iPS cells as single cells

FreSR™-S
50 mL
331 USD
Catalog # 05859

Animal component-free medium for freezing ES and iPS cells as single cells

Product Advantages

• Defined, serum-free and animal component-free medium for cryopreserving ES/iPS cells as single cells
• Quickly recover ES/iPS cell colonies after thawing
• Reproducibly high recovery rates
• Optimized for cryopreserving ES/iPS cells cultured in TeSR™ maintenance media
• Preserves ES/iPS cell pluripotency and expansion capacities
• Convenient, ready-to-use format

Overview

FreSR™-S is a defined, serum-free, and animal component-free medium for the cryopreservation of human pluripotent stem cells (hPSCs) as single cells. This complete and ready-to-use medium is recommended for hPSCs cultured in mTeSR™1, mTeSR™ Plus, TeSR™2, or TeSR™-E8™. Cryopreserved hPSCs should be stored at -135°C (liquid nitrogen) or colder.
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cryopreservation
Brand
mFreSR, TeSR
Area of Interest
Stem Cell Biology
Formulation
Animal Component-Free, Serum-Free

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
FreSR™-S
Catalog #
05859
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
FreSR™-S
Catalog #
05859
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

High Viability and Recovery of Cells Stored in FreSR™-S

Figure 1. High Viability and Recovery of Cells Stored in FreSR™-S

hPSCs cryopreserved as single cells using FreSR™-S have (A) higher post-thaw recovery (number of cells recovered / number of cells frozen) and (B) maintain higher viability (number of live cells / total number of cells) compared to competitor medium. All data values are plotted as percentages, (n=18, p < 0.0001 for each).

More Vials Frozen and Banked With FreSR™-S

Figure 2. More Vials Frozen and Banked With FreSR™-S

hPSCs are cryopreserved in FreSR™-S at lower cell density compared to traditional methods. Graph indicates the number of vials cryopreserved for each well of a 6 well plate that is harvested.
Note: 1 vial is typically thawed and seeded directly into 1 well of a six well plate. Cells should be cultured as aggregates after thawing.

hPSCs Frozen and Thawed as Single Cells with FreSR™-S Display a Normal Karyotype

Figure 3. hPSCs Frozen and Thawed as Single Cells with FreSR™-S Display a Normal Karyotype

Karyograms of (A-B) WLS-4D1 hiPS cells and (C-D) H1 hES cells that were frozen and thawed as single cells using FreSR™-S. (A, C) Thawed cells were seeded into culture containing TeSR™-E8™ medium and 10 µM Y-27632 and maintained as aggregates for five passages. (B,D) hPSCs were also subjected to a second freeze-thaw cycle as single cells with FreSR™-S and cultured for five passages as aggregates prior to collection of karyotype data.

Publications (1)

Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Draper JS et al. Nature biotechnology 2004 JAN

Abstract

We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.

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