Human Pluripotent Stem Cell Research

Maintenance of high quality human embryonic stem (ES) and induced pluripotent stem (iPS) cells is critical to success in all applications of PSC research. See MoreSpecific culture conditions are required in order to maintain undifferentiated ES and iPS cells that are capable of self-renewal. Use our defined, feeder-free TeSR-based media , xeno-free cell attachment substrates, and animal component-free passaging reagents, to culture hPSCs and minimize variation in your research. For long-term storage, our suite of cryopreservation media is designed to maintain high viability and maximize hPSC recovery after thawing. For help with your hPSC workflow decision making, use our infographics to find the right reagents for you.

Learn More

Technical Bulletin Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Tech Tip Coating Plates with Matrigel™ for Pluripotent Stem Cell Culture
Tech Tip Tap Your Way to Easy Pluripotent Stem Cell Passaging Using ReLeSR™
Wallchart Stem Cell States: Naive to Primed Pluripotency
Webinar (3:11) Choosing the Right Medium for Your hPSC Culture

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Products

Matrices and Substrates 4 (1)

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0.1% Gelatin in Water

Coating for cultureware

Tissue and Cell Culture Dissociation Reagents 4 (3)

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Collagenase Type IV (1 mg/mL)

Cell dissociation reagent
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Dispase (1 U/mL)

1 U/mL dispase in DMEM/F-12
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Dispase, ACF

Animal component-free non-specific protease

Cell Storage Media 4 (3)

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CryoStor® CS2

Animal component-free, defined cryopreservation medium with 2% DMSO
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CryoStor® CS5

Animal component-free, defined cryopreservation medium with 5% DMSO
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CryoStor® CS10

Animal component-free, defined cryopreservation medium with 10% DMSO

Antibodies 4 (4)

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Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-54 (tissue non-specific alkaline phosphatase)
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Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-49 (tissue non-specific alkaline phosphatase)
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Anti-Human SSEA-4 Antibody, Clone MC-813-70

Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4
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Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R

Mouse monoclonal IgM antibody against human, rhesus, rabbit TRA-1-60 (podocalyxin)

Small Molecules 4 (24)

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1-Azakenpaullone

Inhibits glycogen synthase kinase 3β (GSK3β)
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RSC-133

Epigenetic modifier; Inhibits DNA methyltransferase (DNMT)
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Gatifloxacin

Antibiotic; Inhibits bacterial DNA gyrase and topoisomerase IV
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ID-8

DYRK pathway inhibitor

Cultureware and General Supplies 4 (3)

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Axygen® Thin-Wall PCR Tubes

Non-sterile, clear, RNase/DNase-free, polypropylene conical tubes with caps
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Costar® Microcentrifuge Tubes

Non-sterile, clear, polypropylene snap-cap microcentifuge tubes
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Reversible Strainers

Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

Buffers and Solutions 4 (1)

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D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

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