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The CRISPR-Cas9 genome editing system enables high-efficiency genome editing to generate functional gene-edited cells. Bring this system and its best practices to your lab by registering for this three-day course consisting of live-streamed, interactive lectures and laboratory demonstrations. Guided by STEMCELL Technologies’ in-house pluripotent stem cell experts, you will gain the skills to successfully derive and maintain CRISPR-Cas9 genome-edited human pluripotent stem cells (hPSCs).
Learning Outcomes:
Describe the evolution of genome editing techniques and hPSC culture
Know the advantages and mechanism of CRISPR-Cas9
Understand the potential and challenges associated with CRISPR-Cas9 in hPSCs
Be familiar with a typical hPSC genome editing workflow (knock out and insertion of point mutations by knock-in using an ssODN template)
Derivation of clonal lines
Distinguish CRISPR-Cas9 guide RNA (gRNA) formats
Understand and apply the principles of gRNA design to design a gRNA sequence and knock-in DNA donor template for point mutations
Identify contributing factors to off-target effects of CRISPR-Cas9
Understand considerations for knockout and knock-in experiments
Discern the benefits and drawbacks of different methods of determining genome editing efficiencies to identify the most suitable method for your research objectives/applications
Understand the basics of hPSC cell quality and considerations for hPSC research
As part of the course, you will receive:
An electronic copy of the course book
Certificate of course completion from STEMCELL Technologies
Post-training support from the trainers and STEMCELL’s Scientific Support team
Space is limited, please apply early for the best chance of securing a seat for your desired date. Registration closes one week before the course date.
Subtype
Genome Editing, Live Virtual Training
Cell Type
Pluripotent Stem Cells
Area of Interest
Cell Line Development, Disease Modeling, Stem Cell Biology, Cell Therapy Development
Lab Live-Stream 2: hPSC Single-Cell Morphology, Taking Samples for Analysis, and hPSC Cloning (1.5 h)
Day 3 (3 hrs, including break and wrap-up/Q&A)
Lecture 3: Analysis of Editing Efficiency, hPSC Cloning, and Quality Control (2.5 h)
Policy
Payment information is required to complete course registration; you will not be charged until your registration is confirmed. Registration and acceptance into the course is subject to review by STEMCELL Technologies. You will be notified within one week of submission of your status.
Applications
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
Live Virtual Training: Genome-Editing Pluripotent Stem Cells
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
