Genome Editing
The use of genome editing tools to generate specific changes to the DNA sequence of human pluripotent stem cells (hPSCs), by either introducing or correcting mutations, provides more accurate modeling of human disease when the gene-edited hPSCs are subsequently differentiated into human-derived tissues.
Products
Cell Culture Media and Supplements 4 (8)
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StemSpan™-AOF
cGMP medium, for culture and expansion of human hematopoietic cells -
IntestiCult™ Organoid Growth Medium (Human)
Cell culture medium for establishment and maintenance of human intestinal organoids -
CloneR™2
Defined supplement for improving survival of human ES and iPS cells in single-cell workflows -
ImmunoCult™-XF T Cell Expansion Medium
Serum-free and xeno-free medium for the expansion of T cells
Cell Engineering and Molecular Tools 4 (11)
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ArciTect™ High-Fidelity DNA Polymerase Kit
Polymerase, buffers, and dNTPs for high-fidelity PCR amplification -
CellPore™ Transfection Kit 300
Transfection & intracellular delivery kit for cell engineering applications -
ArciTect™ Cas9-eGFP Nuclease
Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing -
ArciTect™ sgRNA
Custom-designed single guide RNA for CRISPR-Cas9 genome editing
Instruments and Software 4 (1)
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CellPore™ Transfection System
Cell mechanoporation system for transfection & intracellular delivery
Primary and Cultured Cells 4 (46)
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Diseased Human Peripheral Blood Products, Graves' Disease
Primary human cells, fresh or frozen -
Diseased Human Peripheral Blood Products, Hidradenitis Suppurativa
Primary human cells, fresh or frozen -
Diseased Human Peripheral Blood Products, Crohn's Disease
Diseased primary human cells, fresh or frozen -
Diseased Human Peripheral Blood Products, Vasculitis
Primary human cells, fresh or frozen
Training and Education 4 (1)
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Live Virtual Training: Genome-Editing Pluripotent Stem Cells
Learn best practices for deriving and maintaining CRISPR-Cas9 genome-edited hPSCs.
Cultureware and General Supplies 4 (2)
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Costar® Microcentrifuge Tubes
Non-sterile, clear, polypropylene snap-cap microcentrifuge tubes -
Axygen® Thin-Wall PCR Tubes
Non-sterile, clear, RNase/DNase-free, polypropylene conical tubes with caps
Small Molecules 4 (7)
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KU0060648
NHEJ pathway inhibitor; Inhibits DNA-dependent protein kinase (DNA-PK) -
Nocodazole
Microtubule polymerization inhibitor; Inhibits beta-tubulin -
NU7441
NHEJ pathway inhibitor; Inhibits DNA-dependent protein kinase (DNA-PK) -
RS-1
HDR pathway enhancer; Enhances RAD51
