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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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ArciTect™ High-Fidelity DNA Polymerase Kit is optimized for robust, high-fidelity DNA amplification (> 50X higher than Taq). It may be used in applications requiring ultra-low error rates, such as detection of genome editing with ArciTect™ T7 Endonuclease I (refer to the Technical Bulletin: Evaluation of Genome Editing), as well as sequencing, cloning/subcloning, synthetic biology, and SNP analysis. ArciTect™ High-Fidelity DNA Polymerase is a fusion protein with a double-stranded DNA-binding domain and a Pyrococcus-like proofreading polymerase domain.
ArciTect™ High-Fidelity DNA Polymerase Kit includes ArciTect™ High-Fidelity DNA Polymerase, dNTP Mix (10 mM; containing dATP, dCTP, dGTP, and dTTP sodium salts), and two buffers: ArciTect™ High-Fidelity Buffer (for standard high-fidelity reactions) and ArciTect™ High GC Content Buffer (for difficult-to-amplify templates that are rich in G and C bases). Reactions using ArciTect™ High GC Content Buffer have increased sensitivity with a slightly higher error rate. All components are animal component-free.
ArciTect™ High-Fidelity DNA Polymerase exhibits a 50- to 60-fold lower error rate than standard Taq polymerase using the lacl mutagenesis assay. It has an extension rate of 67 nucleotides per second and can successfully amplify long targets (up to 5 kb human genomic DNA and 8 kb of lambda DNA). This enzyme generates blunt-end products.
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
For estimation of CRISPR-Cas9 genome editing efficiency
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ArciTect™ High-Fidelity DNA Polymerase Kit
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Legal Statement:
Sale of this product is covered under at least US Patent Nos. 6,627,424, 7,541,170, 7,560,260, and corresponding patents in other countries or other patents pending and all applicable STEMCELL Terms and Conditions of Sale. This product can be used solely for DNA sequencing, DNA micro-array and conventional PCR applications in the life science research field, but not real-time PCR or digital PCR.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.