ArciTect™ Human HPRT Positive Control Kit

Positive control for CRISPR-Cas9 genome editing
ArciTect™ Human HPRT Positive Control Kit

Positive control for CRISPR-Cas9 genome editing

1 Kit
Catalog # 76013
264 USD
Required Products
  1. ArciTect™ Cas9 Nuclease
    ArciTect™ Cas9 Nuclease

    Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

  2. ArciTect™ High-Fidelity DNA Polymerase Kit
    ArciTect™ High-Fidelity DNA Polymerase Kit

    Polymerase, buffers, and dNTPs for high-fidelity PCR amplification

  3. ArciTect™ T7 Endonuclease I Kit|76021
    ArciTect™ T7 Endonuclease I Kit

    For estimation of CRISPR-Cas9 genome editing efficiency

  4. Nuclease-Free Water|79001
    Nuclease-Free Water

    DNase- and RNase-free water for molecular biology applications

Overview

ArciTect™ Human HPRT Positive Control Kit is designed as a positive control for experiments using the ArciTect™ CRISPR-Cas9 genome editing system. The kit comprises ArciTect™ Human HPRT crRNA (2 nmol), ArciTect™ Human HPRT Primer Mix (2 nmol), ArciTect™ tracrRNA (5 nmol), and ArciTect™ Annealing Buffer (5X). ArciTect™ Human HPRT crRNA first requires annealing to ArciTect™ tracrRNA (included in the kit). This must then be combined with an ArciTect™ Cas9 Nuclease (e.g. Catalog #76002) to form a ribonucleoprotein complex. ArciTect™ Human HPRT Primer Mix can be used to amplify genomic DNA isolated from a population of transfected cells, which can subsequently be used in a T7 endonuclease I assay to determine cleavage efficiency.
This kit has been tested and validated for use with the ArciTect™ line of genome editing products. HPRT, or hypoxanthine phosphoribosyltransferase, is a housekeeping gene and a commonly used control. The kit can be used to optimize transfection protocols and act as a positive control that can be used alongside custom ArciTect™ crRNAs (e.g. Catalog #76010).
Components
  • ArciTect™ Human HPRT crRNA, 2 nmol
  • ArciTect™ Human HPRT Primer Mix, 2 nmol
  • ArciTect™ tracrRNA, 5 nmol
  • ArciTect™ Annealing Buffer (5X), 100 µL
Cell Type
Pluripotent Stem Cells
Species
Human
Brand
ArciTect
Area of Interest
Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ArciTect™ Human HPRT Positive Control Kit
Catalog #
76013
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ArciTect™ Human HPRT Positive Control Kit
Catalog #
76013
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ArciTect™ Human HPRT Positive Control Kit
Catalog #
76013
Lot #
All
Language
English

Educational Materials (6)

Brochure
ArciTect™ CRISPR-Cas9 Genome Editing System
Technical Bulletin
Genome Editing with Direct Cas9 RNP Delivery Design Considerations
Technical Bulletin
Evaluation of Genome Editing
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Technical Bulletin
Genome Editing of Human Pluripotent Stem Cells Using the ArciTect™ CRISPR-Cas9 System
Webinar
CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells
55:51
CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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