ArciTect™ Human HPRT Positive Control Kit
Positive control for CRISPR-Cas9 genome editing
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ArciTect™ Human HPRT Positive Control Kit -
Label for ArciTect™ Human HPRT Positive Control Kit -
Label for ArciTect™ Human HPRT Positive Control Kit -
Label for ArciTect™ Human HPRT Positive Control Kit -
Label for ArciTect™ Human HPRT Positive Control Kit
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Required Products
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Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing -
ArciTect™ High-Fidelity DNA Polymerase Kit
Polymerase, buffers, and dNTPs for high-fidelity PCR amplification -
ArciTect™ T7 Endonuclease I Kit
For estimation of CRISPR-Cas9 genome editing efficiency -
DNase- and RNase-free water for molecular biology applications
Overview
ArciTect™ Human HPRT Positive Control Kit is designed as a positive control for experiments using the ArciTect™ CRISPR-Cas9 genome editing system. The kit comprises ArciTect™ Human HPRT crRNA (2 nmol), ArciTect™ Human HPRT Primer Mix (2 nmol), ArciTect™ tracrRNA (5 nmol), and ArciTect™ Annealing Buffer (5X). ArciTect™ Human HPRT crRNA first requires annealing to ArciTect™ tracrRNA (included in the kit). This must then be combined with an ArciTect™ Cas9 Nuclease (e.g. Catalog #76002) to form a ribonucleoprotein complex. ArciTect™ Human HPRT Primer Mix can be used to amplify genomic DNA isolated from a population of transfected cells, which can subsequently be used in a T7 endonuclease I assay to determine cleavage efficiency.
This kit has been tested and validated for use with the ArciTect™ line of genome editing products. HPRT, or hypoxanthine phosphoribosyltransferase, is a housekeeping gene and a commonly used control. The kit can be used to optimize transfection protocols and act as a positive control that can be used alongside custom ArciTect™ crRNAs (e.g. Catalog #76010).
This kit has been tested and validated for use with the ArciTect™ line of genome editing products. HPRT, or hypoxanthine phosphoribosyltransferase, is a housekeeping gene and a commonly used control. The kit can be used to optimize transfection protocols and act as a positive control that can be used alongside custom ArciTect™ crRNAs (e.g. Catalog #76010).
Components:
- ArciTect™ Human HPRT crRNA, 2 nmol
- ArciTect™ Human HPRT Primer Mix, 2 nmol
- ArciTect™ tracrRNA, 5 nmol
- ArciTect™ Annealing Buffer (5X), 100 µL
Cell Type:
Pluripotent Stem Cells
Species:
Human
Brand:
ArciTect
Area of Interest:
Stem Cell Biology
Related Products
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For purification of genomic DNA from cells or tissue -
ArciTect™ T7 Endonuclease I Kit
For estimation of CRISPR-Cas9 genome editing efficiency -
For extraction of DNA from agarose gels, or clean-up of PCR products and restriction enzyme digestions -
For size determination and tracking of double-stranded DNA products by gel electrophoresis
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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The purchase of the ArciTect™ products conveys to the purchaser the limited, non-transferable right to use, in accordance with all applicable laws and regulations, the ArciTect™ products and any related material solely for research purposes only, not for any clinical, human, agricultural, veterinary, livestock, or commercial purpose, with no warranty (express or implied) from the owners or assignees of the Patents or STEMCELL Technologies each of whom expressly disclaim any warranty regarding results obtained through the use of the ArciTect™ products, and without any exception the owners or assignees of the Patents or STEMCELL Technologies, or as applicable a director, trustee, officer, employee, agent, faculty, official investigator or student thereof, will not suffer or be exposed to any liability, damages, loss, or expense of any kind whatsoever arising from or related to the use of the ArciTect™ products by a purchaser of same. Distribution of ArciTect™ products by STEMCELL Technologies is covered under at least US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents (“Patents”).
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