Gel and PCR Clean-up Kit

For extraction of DNA from agarose gels, or clean-up of PCR products and restriction enzyme digestions
Gel and PCR Clean-up Kit

For extraction of DNA from agarose gels, or clean-up of PCR products and restriction enzyme digestions

250 Preps
Catalog # 79030
606 USD
Required Products
  1. Filtered Pipette Tips, 1000 µL
    Corning® Filtered Pipette Tips

    Sterile, polypropylene filtered pipette tips

  2. Costar® Microcentrifuge Tubes, 0.65 mL
    Costar® Microcentrifuge Tubes

    Non-sterile, clear, polypropylene snap-cap microcentifuge tubes

Overview

The Gel and PCR Clean-up Kit uses a membrane-based system to quickly purify DNA fragments of 100 bp to 10 kb in length from polymerase chain reaction (PCR), restriction enzyme digestion, or excised agarose gel fragments (up to 350 mg). The purified DNA is directly suitable for applications such as automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, or in vitro transcription. Up to 40 µg of DNA can be processed per column in as little as 20 minutes and eluted in volumes as low as 15 µL, with up to 95% recovery depending on DNA fragment length. The Gel and PCR Clean-up Kit can be used to purify linear DNA fragments, supercoiled plasmid DNA, or single-stranded DNA (circular or linear).
Components
  • Gel and PCR Clean-up Kit (Catalog #79030)
    • DNA Minicolumns, 250x
    • Collection Tubes, 250x
    • Membrane Wash Solution, 75 mL
    • Membrane Binding Solution, 100 mL
    • Nuclease-Free Water, 13 mL

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Gel and PCR Clean-up Kit
Catalog #
79030
Lot #
All
Language
English
Document Type
Manual
Product Name
Gel and PCR Clean-up Kit
Catalog #
79030
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Gel and PCR Clean-up Kit
Catalog #
79030
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Gel and PCR Clean-up Kit
Catalog #
79030
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Gel and PCR Clean-up Kit
Catalog #
79030
Lot #
All
Language
English

Educational Materials (1)

Technical Bulletin
Protocol for DNA Purification From a Gel Slice or PCR Amplification Product

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