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ArciTect™

CRISPR-Cas9 Genome Editing Precision for Cell Biologists

The ArciTect™ family of products for CRISPR-Cas9 genome editing provides you with a rapid, flexible and precise system to modify the genome as you see fit. The guide RNA (gRNA) complex, consisting of crRNA and tracrRNA, can be customized using the online order tool. A protocol for the efficient genome editing of human embryonic stem (hES) cells or human induced pluripotent stem (hiPS) cells has been optimized using the ArciTect™ product family.

Reduce Off-Target Effects

The ArciTect™ product family is a ribonucleoprotein (RNP)-based Cas9 genome editing system. Unlike previous CRISPR technologies which utilize plasmid or mRNA-based systems, the ArciTect™ system shows timely degradation of the RNP complex to minimize cleavage of off-target regions.

Why Use ArciTect™?

  • Design crRNA to target your sequence of interest.
  • Multiple variations of Cas9 to suit your specific genome editing needs.
  • No need for transcription and translation.
  • Timely degradation of the RNP complex to minimize potential off-target cutting.

Figure 1. Genome Editing Workflow for Human ES and iPS Cells.

Table 1. Comparison Between Different CRISPR Methods. 1


Guide RNA (gRNA)

Features:

  • Two-part gRNA: ArciTect™ tracrRNA is compatible with all ArciTect™ crRNA
  • Compatible with all Cas9 nucleases

Applications:

  • Use ArciTect™ crRNA to guide the Cas9 nuclease to specific target location

Cas9

Features:

  • RNP complex is active immediately following transfection
  • All versions of Cas9 contain nuclear localization signals for rapid translocation into the nucleus

Applications:

  • Use ArciTect™ Cas9 Nuclease to generate double-strand breaks at specific locations in the genome
  • Use ArciTect™ Cas9-eGFP Nuclease to optimize transfection conditions or sort cells following transfection

Human HPRT

Features:

  • Validated for use with ArciTect™ family of products for genome editing

Applications:

  • Use ArtiTect™ Human HPRT crRNA and Primer Mix as a positive control to optimize transfection protocols
  • ArciTect™ Human HPRT Primer Mix can be used in a T7 endonuclease assay to assess cleavage efficiency
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References

  1. Liang X et al. (2015) Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. J Biotechnol. 208: 44-53.