CRISPR-Cas9 Genome Editing Precision for Cell Biologists
The ArciTect™ family of products for CRISPR-Cas9 genome editing provides you with a rapid, flexible and precise system to modify the genome as you see fit. The guide RNA (gRNA) complex, consisting of crRNA and tracrRNA, can be customized using the online order tool. A protocol for the efficient genome editing of human embryonic stem (hES) cells or human induced pluripotent stem (hiPS) cells has been optimized using the ArciTect™ product family.
Reduce Off-Target Effects
The ArciTect™ product family is a ribonucleoprotein (RNP)-based Cas9 genome editing system. Unlike previous CRISPR technologies which utilize plasmid or mRNA-based systems, the ArciTect™ system shows timely degradation of the RNP complex to minimize cleavage of off-target regions.
Why Use ArciTect™?
- Design crRNA to target your sequence of interest.
- Multiple variations of Cas9 to suit your specific genome editing needs.
- No need for transcription and translation.
- Timely degradation of the RNP complex to minimize potential off-target cutting.
Figure 1. Genome Editing Workflow for Human ES and iPS Cells.
Table 1. Comparison Between Different CRISPR Methods. 1
Guide RNA (gRNA)
- Two-part gRNA: ArciTect™ tracrRNA is compatible with all ArciTect™ crRNA
- Compatible with all Cas9 nucleases
- Use ArciTect™ crRNA to guide the Cas9 nuclease to specific target location
- RNP complex is active immediately following transfection
- All versions of Cas9 contain nuclear localization signals for rapid translocation into the nucleus
- Use ArciTect™ Cas9 Nuclease to generate double-strand breaks at specific locations in the genome
- Use ArciTect™ Cas9-eGFP Nuclease to optimize transfection conditions or sort cells following transfection
- Validated for use with ArciTect™ family of products for genome editing
- Use ArtiTect™ Human HPRT crRNA and Primer Mix as a positive control to optimize transfection protocols
- ArciTect™ Human HPRT Primer Mix can be used in a T7 endonuclease assay to assess cleavage efficiency
- Liang X et al. (2015) Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. J Biotechnol. 208: 44-53.