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ArciTect™ tracrRNA Kit

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

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From: 95 USD

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Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing
From: 95 USD

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Overview

ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing. ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation. ArciTect™ tracrRNA is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation. It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.
The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA. ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2 nmol (Catalog #76010), 10 nmol (Catalog #76011), and 20 nmol (Catalog #76012) sizes. ArciTect™ Cas9 Nuclease is also available separately (Catalog #76001).
Components:
  • ArciTect™ tracrRNA Kit, 5 nmol (Catalog #76016)
    •  ArciTect™ tracrRNA, 5 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
 
  • ArciTect™ tracrRNA Kit, 10 nmol (Catalog #76017)
    • ArciTect™ tracrRNA, 10 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
 
  • ArciTect™ tracrRNA Kit, 20 nmol (Catalog #76018)
    • ArciTect™ tracrRNA, 20 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
Cell Type:
Pluripotent Stem Cells
Species:
Human
Brand:
ArciTect
Area of Interest:
Stem Cell Biology

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.