ArciTect™ tracrRNA Kit

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

ArciTect™ tracrRNA Kit

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

From: 118 USD
Catalog #
76016_C
Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

What's Included

  • ArciTect™ tracrRNA Kit, 5 nmol (Catalog #76016)
    •  ArciTect™ tracrRNA, 5 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
 
  • ArciTect™ tracrRNA Kit, 10 nmol (Catalog #76017)
    • ArciTect™ tracrRNA, 10 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
 
  • ArciTect™ tracrRNA Kit, 20 nmol (Catalog #76018)
    • ArciTect™ tracrRNA, 20 nmol
    • ArciTect™ Annealing Buffer (5X), 100 µL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing. ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation. ArciTect™ tracrRNA is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation. It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.
The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA. ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2 nmol (Catalog #76010), 10 nmol (Catalog #76011), and 20 nmol (Catalog #76012) sizes. ArciTect™ Cas9 Nuclease is also available separately (Catalog #76001).
Cell Type
Pluripotent Stem Cells
Species
Human
Brand
ArciTect
Area of Interest
Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
76016, 76017, 76018
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
76016, 76017, 76018
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
76016, 76017, 76018
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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