Single Guide RNA (sgRNA) Design and Order Tool for CRISPR-Cas9 Genome Editing

ArciTect™ sgRNA is a custom single guide RNA (sgRNA) for CRISPR-Cas9 genome editing. ArciTect™ sgRNA contains a user-specified 19- to 21-base sequence complementary to the target genomic location. ArciTect™ sgRNA must be designed directly upstream of a protospacer adjacent motif (PAM) site (5’-NGG-3’). It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.

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Please note that the design tool is currently not compatible with Internet Explorer browsers. Please use this order form instead.


Search predesigned ArciTect™ sgRNA sequence by gene or enter your designed sequence by selecting the appropriate option below.



Guide Search and Ranking Algorithm

This tool uses best practices and the latest computational tools to deliver the optimal CRISPR RNA (crRNA) or single guide RNA (sgRNA) sequence for every gene in the human and mouse genomes. Learn More.