ArciTect™

The ArciTect™ product family is a CRISPR-Cas9 genome editing system that uses ribonucleoprotein (RNP) complexes composed of purified Cas9 protein and custom synthetic guide RNA. Compared to previous technologies that utilize plasmid or mRNA-based systems, an RNP-based system enables efficient delivery and expression of CRISPR machinery in difficult-to-manipulate cells, including stem and primary cells. Once inside the cell, the RNP complexes will not induce the cellular immune response and will degrade in a timely manner to reduce off-target effects (Table 1). With validated reagents and protocols, the ArciTect™ system enables you to perform high-efficiency genome editing and generate functional gene-edited cells in your own lab.

Overcome Hurdles in Efficiency

Overcome challenges in efficient delivery and expression of CRISPR machinery using an RNP-based CRISPR-Cas9 system. ArciTectTM enables researchers to perform high-efficiency genome editing in difficult-to-manipulate cell types, including stem and primary cells.

Why Use ArciTect™?

  • Maximize delivery and expression in difficult-to-manipulate cell types by using RNP complexes.
  • Get to your results faster with ready-to-use purified Cas9 proteins and synthetic guide RNAs.
  • Meet your specific genome editing needs with multiple variations of Cas9.
  • Minimize potential off-target cutting with timely degradation of the RNP complex.

Figure 1. Genome Editing Workflow

Table 1. Comparison Between Different CRISPR Methods. 1


Protocols and Data

Explore step-by-step instructions for performing high-efficiency genome editing using CRISPR-Cas9 in a variety of cell lines, including stem and primary cell types. The protocols are optimized and validated with a case study and supporting data, and include important cell culture considerations and methods to evaluate editing efficiency.

Human Pluripotent Stem Cells (hPSCs)

Gene knockout and knock-in in hPSCs using electroporation and chemical transfection.
Case study: Knockout and Knock-in optimization through GFP to BFP conversion

Read the Technical Bulletin >

Human Primary T Cells

Gene knockout in primary human T cells using electroporation.
Case study: Evaluation of optimal culture methods for high-efficiency TRAC knockout

Read the Technical Bulletin >

CD34+ Human Hematopoietic Stem and Progenitor Cells (HSPCs)

Gene knockout in HSPCs using electroporation.
Case study: Evaluation of optimal culture methods for high-efficiency genome editing in CD34+ HSPCs

Read the Technical Bulletin >

Products

Guide RNA (gRNA)

Features:

  • Two-part gRNA: ArciTect™ tracrRNA is compatible with all ArciTect™ crRNA
  • Compatible with all Cas9 nucleases

Applications:

  • Use ArciTect™ crRNA to guide the Cas9 nuclease to specific target location

Cas9

Features:

  • RNP complex is active immediately following transfection
  • All versions of Cas9 contain nuclear localization signals for rapid translocation into the nucleus

Applications:

  • Use ArciTect™ Cas9 Nuclease to generate double-strand breaks at specific locations in the genome
  • Use ArciTect™ Cas9-eGFP Nuclease to optimize transfection conditions or sort cells following transfection

Human HPRT

Features:

  • Validated for use with ArciTect™ family of products for genome editing

Applications:

  • Use ArtiTect™ Human HPRT crRNA and Primer Mix as a positive control to optimize transfection protocols
  • ArciTect™ Human HPRT Primer Mix can be used in a T7 endonuclease assay to assess cleavage efficiency
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References

  1. Liang X et al. (2015) Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. J Biotechnol. 208: 44-53.