Considerations for High-Efficiency Genome Editing of Human Pluripotent Stem Cells
Genome editing of hPSCs allows for the generation of isogenic hPSC lines for disease models, by controlling for variation in genetic background between individuals. In this seminar, Ashley reviews STEMCELL’s recommended tools for efficient Cas9 genome editing, and reviews the challenges associated with working with ES/iPS cells as well as tips for overcoming them and maintaining cell quality throughout a project.
Publish Date:
May 29, 2020
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Healthy Control Human iPSC Line, Female, SCTi003-A
Human pluripotent stem cell line, frozen -
Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing -
cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells -
Defined supplement for single-cell cloning of human ES and iPS cells
