Considerations for High-Efficiency Genome Editing of Human Pluripotent Stem Cells
Genome editing of hPSCs allows for the generation of isogenic hPSC lines for disease models, by controlling for variation in genetic background between individuals. In this seminar, Ashley reviews STEMCELL’s recommended tools for efficient Cas9 genome editing, and reviews the challenges associated with working with ES/iPS cells as well as tips for overcoming them and maintaining cell quality throughout a project.
Publish Date:
May 29, 2020
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Explore Our Products
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Defined supplement for improving survival of human ES and iPS cells in single-cell workflows -
Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing -
STEMdiff™ Trilineage Differentiation Kit
Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers -
ArciTect™ Human CRISPR Optimization Kit
Complete kit for optimization of genome editing in human cells
