ArciTect™ crRNA is a custom CRISPR RNA (crRNA), one of two synthetic RNA components required to make a guide RNA (gRNA) template for CRISPR-Cas9 genome editing. ArciTect™ crRNA contains a user-specified 19 - 21 base sequence complementary to the genomic location to be cleaved, and a linker sequence complementary to ArciTect™ tracrRNA (Catalog #76016), allowing fast and efficient gRNA duplex formation. ArciTect™ crRNA should be designed so that it is directly upstream of a protospacer adjacent motif (PAM) site (NGG). It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.
This tool uses best practices and the latest computational tools to deliver the optimal CRISPR RNA (crRNA) or single guide RNA (sgRNA) sequence for every gene in the human and mouse genomes. Learn More.
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