CRISPR RNA (crRNA) Design and Order Tool

ArciTect™ crRNA is a custom CRISPR RNA (crRNA), one of two synthetic RNA components required to make a guide RNA (gRNA) template for CRISPR-Cas9 genome editing. ArciTect™ crRNA contains a user-specified 19 - 21 base sequence complementary to the genomic location to be cleaved, and a linker sequence complementary to ArciTect™ tracrRNA (Catalog #76016), allowing fast and efficient gRNA duplex formation. ArciTect™ crRNA should be designed so that it is directly upstream of a protospacer adjacent motif (PAM) site (NGG). It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.

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Please note that the design tool is currently not compatible with Internet Explorer browsers. Please use this order form instead.


Search predesigned ArciTect™ crRNA sequence by gene or enter your designed sequence by selecting the appropriate option below.



Guide Search and Ranking Algorithm

This tool uses best practices and the latest computational tools to deliver the optimal CRISPR RNA (crRNA) or single guide RNA (sgRNA) sequence for every gene in the human and mouse genomes. Learn More.